Date published: 2025-9-7

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Fibrillarin 抗体 (G-8): sc-374022

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说明书
  • Fibrillarin 抗体 G-8 是小鼠单克隆 IgG1 κ,Fibrillarin抗体, 在25篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Fibrillarin的内部区域内的氨基酸99-127之间
  • Fibrillarin 抗体 (G-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Fibrillarin; 也和以下物种反应,包括: 和 canine, bovine and porcine
  • 抗Fibrillarin抗体(G-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Fibrillarin (G-8): sc-374022免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 Fibrillarin 抗体 (G-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Fibrillarin 抗体 (G-8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

纤维蛋白抗体(G-8)是一种IgG1 κ小鼠单克隆纤维蛋白抗体(也称为FBL抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的纤维蛋白蛋白。纤维蛋白抗体(G-8)既可作为非偶联抗纤维蛋白抗体形式,也可作为多种偶联形式的抗纤维蛋白抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。纤维蛋白是一种广泛存在的基本非组蛋白,专门定位于核仁的纤维状区域,包括致密的纤维状中心和纤维状中心区域。纤维蛋白也是一种在HeLa细胞、3T3细胞和人外周血淋巴细胞中表达的蛋白质。在有丝分裂中期和后期,纤维蛋白位于假定的染色体核仁区域(NORs)。在末期,纤维蛋白是新形成核仁部位的早期标记物。纤维蛋白的结构包括一个RNA结合域和一个RNP共识序列,这与纤维蛋白与U3小核仁RNA的结合是一致的。纤维蛋白参与核仁中rRNA转录本的处理。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Fibrillarin 抗体 (G-8) 参考文献:

  1. 纤毛素对小鼠的早期发育至关重要,是内含子编码的小核仁核糖核酸积累所必需的。  |  Newton, K., et al. 2003. Mol Cell Biol. 23: 8519-27. PMID: 14612397
  2. HeLa细胞的正常核形态和细胞生长需要一种核仁蛋白--纤丝蛋白。  |  Amin, MA., et al. 2007. Biochem Biophys Res Commun. 360: 320-6. PMID: 17603021
  3. 人类核仁蛋白纤维蛋白的进化保护及其在酵母中的功能表达  |  Jansen, RP., et al. 1991. J Cell Biol. 113: 715-29. PMID: 2026646
  4. 纤丝蛋白:通过自身免疫血清发现的一种新的核仁蛋白。  |  Ochs, RL., et al. 1985. Biol Cell. 54: 123-33. PMID: 2933102
  5. 依赖于 SIRT7 的纤连蛋白去乙酰化在细胞周期中控制组蛋白 H2A 甲基化和 rRNA 合成  |  Iyer-Bierhoff, A., et al. 2018. Cell Rep. 25: 2946-2954.e5. PMID: 30540930
  6. 发现针对人类甲基转移酶 Fibrillarin 的辅因子竞争性抑制剂。  |  Shi, Y., et al. 2021. Pharmaceuticals (Basel). 15: PMID: 35056083
  7. RNA 2'-O-Methyltransferase Fibrillarin 通过抑制 I 型干扰素反应促进病毒进入巨噬细胞  |  Li, P., et al. 2022. Front Immunol. 13: 793582. PMID: 35464456
  8. 低水平的核糖体生物生成因子 Fibrillarin 是乳腺癌不良预后的一个新的独立标志。  |  Nguyen Van Long, F., et al. 2022. BMC Cancer. 22: 526. PMID: 35545761
  9. 一种抑制 RNA 沉默的病毒抑制剂可能正瞄准涉及纤丝蛋白的植物防御途径。  |  Pérez-Cañamás, M., et al. 2022. Plants (Basel). 11: PMID: 35893605
  10. 贾第虫的一种保守的核糖体前 RNA 处理蛋白--Fibrillarin。  |  Narcisi, EM., et al. 1998. J Eukaryot Microbiol. 45: 105-11. PMID: 9495039

订购信息

产品名称产品编号规格价格数量收藏夹

Fibrillarin 抗体 (G-8)

sc-374022
200 µg/ml
$316.00

Fibrillarin (G-8): m-IgG Fc BP-HRP 套装

sc-529638
200 µg Ab; 10 µg BP
$354.00

Fibrillarin (G-8): m-IgGκ BP-HRP 套装

sc-522592
200 µg Ab, 40 µg BP
$354.00

Fibrillarin 抗体 (G-8) AC

sc-374022 AC
500 µg/ml, 25% agarose
$416.00

Fibrillarin 抗体 (G-8) HRP

sc-374022 HRP
200 µg/ml
$316.00

Fibrillarin 抗体 (G-8) FITC

sc-374022 FITC
200 µg/ml
$330.00

Fibrillarin 抗体 (G-8) PE

sc-374022 PE
200 µg/ml
$343.00

Fibrillarin 抗体 (G-8) Alexa Fluor® 488

sc-374022 AF488
200 µg/ml
$357.00

Fibrillarin 抗体 (G-8) Alexa Fluor® 546

sc-374022 AF546
200 µg/ml
$357.00

Fibrillarin 抗体 (G-8) Alexa Fluor® 594

sc-374022 AF594
200 µg/ml
$357.00

Fibrillarin 抗体 (G-8) Alexa Fluor® 647

sc-374022 AF647
200 µg/ml
$357.00

Fibrillarin 抗体 (G-8) Alexa Fluor® 680

sc-374022 AF680
200 µg/ml
$357.00

Fibrillarin 抗体 (G-8) Alexa Fluor® 790

sc-374022 AF790
200 µg/ml
$357.00

Fibrillarin (G-8) 中和勝肽

sc-374022 P
100 µg/0.5 ml
$68.00

I was wondering whether you have any information regarding room temp incubation of this antibody (sc-374022) for IF on cells? I'm planning to use this for a drug screen and the system is not amenable to o/n incubation at 4C. Thanks.

Asked by: Ceryl
Thank you for your question. Yes, we do recommend incubating with this antibody for 60 minutes at room temperature. Please refer to our immunofluorescence protocol for more detailed information.
Answered by: Technical Support
Date published: 2019-03-03

What application is the blocking peptide sc-374022 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-15
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Rated 2 out of 5 by from Plasmodium falciparumAntibody did not work as expected against Plasmodium falciparum blood stages in IFA. The G-4 antibody, however, does.
Date published: 2022-11-29
Rated 3 out of 5 by from Works ok for IF.akI got a sample of this antibody. Clear but weak IF with 1:50 dilution.
Date published: 2017-07-27
Rated 5 out of 5 by from great features!Works well for immunofluorescence and Wester blotting in human HT1080 cancer cells and MEF cells.
Date published: 2017-07-10
Rated 5 out of 5 by from an excellent antibody for both WB and IFThis antibody works very well for WB (40 ug U251 Lysate) and IF (formaldehyde-fixed HeLa cells) at 1:500 dilution.
Date published: 2017-07-05
Rated 5 out of 5 by from Great for IF and co-localization experimentsI used a sample of this antibody for co-localization studies in mouse ES cells. The nucleolar staining was very specific and significant. generally pleased with outcome. Will recommend it!
Date published: 2017-06-05
Rated 5 out of 5 by from Broad reactivity.This antibody is capable of detecting Fibrillarin multiple different species. Both staining and blotting assays provide clear and strong signal. This antibody has been used for publishing.
Date published: 2017-01-27
Rated 5 out of 5 by from StrongStrong, clear bands present in Western blot analysis of Fibrillarin expression in HeLa and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Superb immunoperoxidase nucleolar and nuclearSuperb immunoperoxidase nucleolar and nuclear staining in formalin fixed, paraffin-embedded human breast tissue. -SCBT QC
Date published: 2015-04-10
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Fibrillarin 抗体 (G-8) is rated 4.4 out of 5 by 8.
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