Fibrillarin抗体 (G-8) 推荐用于 mouse, rat 和 human 来源的Fibrillarin WB, IP, IF, IHC(P) 和 ELISA检测

Fibrillarin 抗体 (G-8): sc-374022

Fibrillarin抗体 (G-8) is rated 4.7 out of 5 by 7.
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说明书

    • 抗-Fibrillarin 抗体 G-8 是小鼠单克隆 IgG1 κ,Fibrillarin抗体, 在8篇文献中引用,规格为200 µg/ml
    • 特异性抗原位于human物种的Fibrillarin的内部区域内的氨基酸99-127之间
    • 抗-Fibrillarin 抗体 (G-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Fibrillarin; 也和以下物种反应,包括: 和 canine, bovine and porcine
    • 抗-Fibrillarin 抗体 (G-8) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Fibrillarin (G-8): sc-374022免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Fibrillarin Antibody (G-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with Fibrillarin Antibody (G-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

I was wondering whether you have any information regarding room temp incubation of this antibody (sc-374022) for IF on cells? I'm planning to use this for a drug screen and the system is not amenable to o/n incubation at 4C. Thanks.

Asked by: Ceryl
Thank you for your question. Yes, we do recommend incubating with this antibody for 60 minutes at room temperature. Please refer to our immunofluorescence protocol for more detailed information.
Answered by: Technical Support
Date published: 2019-03-03

What application is the blocking peptide sc-374022 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-15
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Rated 3 out of 5 by from Works ok for IF. akI got a sample of this antibody. Clear but weak IF with 1:50 dilution.
Date published: 2017-07-27
Rated 5 out of 5 by from great features! Works well for immunofluorescence and Wester blotting in human HT1080 cancer cells and MEF cells.
Date published: 2017-07-10
Rated 5 out of 5 by from an excellent antibody for both WB and IF This antibody works very well for WB (40 ug U251 Lysate) and IF (formaldehyde-fixed HeLa cells) at 1:500 dilution.
Date published: 2017-07-05
Rated 5 out of 5 by from Great for IF and co-localization experiments I used a sample of this antibody for co-localization studies in mouse ES cells. The nucleolar staining was very specific and significant. generally pleased with outcome. Will recommend it!
Date published: 2017-06-05
Rated 5 out of 5 by from Broad reactivity. This antibody is capable of detecting Fibrillarin multiple different species. Both staining and blotting assays provide clear and strong signal. This antibody has been used for publishing.
Date published: 2017-01-27
Rated 5 out of 5 by from Strong Strong, clear bands present in Western blot analysis of Fibrillarin expression in HeLa and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Superb immunoperoxidase nucleolar and nuclear Superb immunoperoxidase nucleolar and nuclear staining in formalin fixed, paraffin-embedded human breast tissue. -SCBT QC
Date published: 2015-04-10
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