Factor H抗体 (C18/3) 推荐用于 human 来源的Factor H WB, IP 和 IHC(P)检测

Factor H 抗体 (C18/3): sc-47685

Factor H抗体 (C18/3) is rated 4.4 out of 5 by 7.
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说明书

    • 抗-Factor H 抗体 C18/3 是小鼠单克隆 IgG1 κ,Factor H抗体, 在3篇文献中引用,规格为200 µg/ml
    • raised against complement protein factor H purified from human serum
    • Factor H 抗体 (C18/3) 推荐用于WB, IP 和 IHC(P),检测human 来源的 Factor H
    • 抗-Factor H 抗体 (C18/3) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Factor H (C18/3): sc-47685免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP 是 Factor H Antibody (C18/3) for WB and IHC(P)首选应用二抗。这些试剂现在以套装形式提供Factor H Antibody (C18/3) (见下方订购信息).
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Has the epitope on factor H been mapped for this monoclonal antibody? I'm looking for an antibody the recognizes repeats 1-4.

Asked by: Eric Krukonis
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-01-24

Can Factor H (C18/3) sc-47685 antibody be used on an automated IHC stainer, such as Ventana Benchmark Ultra? Do you have any recommended protocols?

Asked by: jdb7928
Thank you for your question. We have not tested this antibody, sc-47685, on an automatic stainer, so I do not have any information regarding that. If you would like to try a sample of this antibody, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com, and we would be glad to send that to you!
Answered by: Tech Service
Date published: 2017-06-27

Can Factor H (C18/3): sc-47685 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Factor H (C18/3): sc-47685 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great antibody I am very happy with this antibody we bought last month
Date published: 2019-09-03
Rated 5 out of 5 by from Works for IHC Received sample for testing. Worked in FFPE human for IHC. Citrate for retrieval. Incubated overnight at 4C at 10ug/mL.
Date published: 2017-11-13
Rated 3 out of 5 by from Did't work in IF of rat brain frozen slice This monocloned antibody did't work in the rat brain frozen slice ,even that was recovered by heat.
Date published: 2017-07-13
Rated 3 out of 5 by from 在大鼠的脑切片免疫荧光实验中没有阳性结合 在大鼠脑冰冻切片(包括用热修复)中,1:25使用该抗体进行免疫荧光实验,没有特异性结合。然后再人源细胞系SY5Y细胞中也进行免疫荧光实验,结合的程度并没有33156好。
Date published: 2017-07-13
Rated 5 out of 5 by from This antibody works well This antibody works well
Date published: 2015-05-28
Rated 5 out of 5 by from Produced wonderful immunoperoxidase cytoplasmic Produced wonderful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human ovary tissue. -SCBT QC
Date published: 2015-05-13
Rated 5 out of 5 by from Good specificity in WB in human whole cell Good specificity in WB in human whole cell lysate and human recombinant Factor H. -SCBT QC
Date published: 2015-03-27
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