Date published: 2025-9-8

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Ep-CAM 抗体 (C-10): sc-25308

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说明书
  • Ep-CAM 抗体 C-10 是小鼠单克隆 IgG1 κ,Ep-CAM抗体, 在83篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Ep-CAM的氨基酸24-93
  • Ep-CAM 抗体 (C-10) 推荐用于WB, IP, IF, IHC(P), FCM 和 ELISA,检测human 来源的 Ep-CAM
  • 抗Ep-CAM抗体(C-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ep-CAM (C-10): sc-25308免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 Ep-CAM 抗体 (C-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ep-CAM 抗体 (C-10) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

Ep-CAM抗体(C-10)是一种小鼠单克隆IgG1κ轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)、流式细胞术(FCM)和酶联免疫吸附测定(ELISA)检测人类来源的Ep-CAM。抗Ep-CAM抗体(C-10)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。上皮细胞粘附分子,也称为肿瘤相关钙信号转导蛋白1和MK-1,是一种重要的单体膜糖蛋白,主要存在于正常人体上皮组织和各种癌细胞中。人类Ep-CAM基因编码一种314个氨基酸的蛋白质,该蛋白质有两种形式,主要形式在用氨基糖苷化抑制剂妥尼卡霉素处理后显著减少。Ep-CAM在细胞粘附中起着至关重要的作用,而细胞粘附对于维持组织完整性和促进细胞交流至关重要。Ep-CAM在多种癌细胞中的过度表达使这种蛋白质成为人类实体肿瘤可视化与治疗干预的有前景的目标。Ep-CAM具有一个细胞外结构域,其中包括两个表皮生长因子样重复序列,后面是一个半胱氨酸缺乏区,该区域对于粘附特性至关重要,从而凸显了Ep-CAM在癌症生物学中的重要性,以及作为肿瘤进展生物标志物的潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ep-CAM 抗体 (C-10) 参考文献:

  1. 小鼠单克隆抗体 FU-MK-1 识别的人类癌症相关糖蛋白抗原的分子鉴定。  |  Tomita, Y., et al. 2000. Jpn J Cancer Res. 91: 231-8. PMID: 10761711
  2. 结肠癌相关糖蛋白 GA733-2(Ep-CAM/EGP40)的低聚物状态及其在 GA733 介导的同型细胞-细胞粘附中的作用。  |  Trebak, M., et al. 2001. J Biol Chem. 276: 2299-309. PMID: 11058587
  3. 表皮生长因子样重复序列介导 Ep-CAM 分子在嗜同性粘附中的横向和互惠相互作用。  |  Balzar, M., et al. 2001. Mol Cell Biol. 21: 2570-80. PMID: 11259604
  4. 小鼠的 cDNA 编码一种泛上皮糖蛋白,它也在浆细胞上表达。  |  Bergsagel, PL., et al. 1992. J Immunol. 148: 590-6. PMID: 1729376
  5. 小鼠胸腺的上皮异质性:一种由囊下上皮和髓质上皮表达的细胞表面糖蛋白。  |  Farr, A., et al. 1991. J Histochem Cytochem. 39: 645-53. PMID: 2016514
  6. 上皮细胞粘附分子-1(ECAM1)对于维持角膜上皮屏障完整性必不可少。  |  Zhou, J., et al. 2016. Cell Biol Int. 40: 49-54. PMID: 26269209
  7. 原代人类肝细胞中的上皮细胞粘附分子片段和信号传导。  |  Gerlach, JC., et al. 2018. J Cell Physiol. 233: 4841-4851. PMID: 29150960
  8. 上皮细胞粘附分子对上皮细胞迁移的调节需要其Claudin-7相互作用域。  |  Barth, AIM., et al. 2018. PLoS One. 13: e0204957. PMID: 30304739
  9. C215 小鼠单克隆抗体识别的人结肠腺癌细胞表面糖蛋白的分离, 部分特征描述和分子克隆。  |  Björk, P., et al. 1993. J Biol Chem. 268: 24232-41. PMID: 7693697
  10. 人类 Ep-CAM 的小鼠同源物是一种同型粘附分子,由胸腺细胞和胸腺上皮细胞表达。  |  Nelson, AJ., et al. 1996. Eur J Immunol. 26: 401-8. PMID: 8617310
  11. 上皮细胞粘附分子(Ep-CAM)可调节由经典粘附蛋白介导的细胞间相互作用。  |  Litvinov, SV., et al. 1997. J Cell Biol. 139: 1337-48. PMID: 9382878
  12. 新西兰黑(NZB)小鼠胸腺上皮细胞粘附分子(EP-CAM)表达异常。  |  Taguchi, N., et al. 1999. J Autoimmun. 13: 393-404. PMID: 10585755

订购信息

产品名称产品编号规格价格数量收藏夹

Ep-CAM 抗体 (C-10)

sc-25308
200 µg/ml
$316.00

Ep-CAM (C-10): m-IgG Fc BP-HRP 套装

sc-528398
200 µg Ab; 10 µg BP
$354.00

Ep-CAM (C-10): m-IgGκ BP-HRP 套装

sc-520770
200 µg Ab, 40 µg BP
$354.00

Ep-CAM 抗体 (C-10) AC

sc-25308 AC
500 µg/ml, 25% agarose
$416.00

Ep-CAM 抗体 (C-10) HRP

sc-25308 HRP
200 µg/ml
$316.00

Ep-CAM 抗体 (C-10) FITC

sc-25308 FITC
200 µg/ml
$330.00

Ep-CAM 抗体 (C-10) PE

sc-25308 PE
200 µg/ml
$343.00

Ep-CAM 抗体 (C-10) Alexa Fluor® 488

sc-25308 AF488
200 µg/ml
$357.00

Ep-CAM 抗体 (C-10) Alexa Fluor® 546

sc-25308 AF546
200 µg/ml
$357.00

Ep-CAM 抗体 (C-10) Alexa Fluor® 594

sc-25308 AF594
200 µg/ml
$357.00

Ep-CAM 抗体 (C-10) Alexa Fluor® 647

sc-25308 AF647
200 µg/ml
$357.00

Ep-CAM 抗体 (C-10) Alexa Fluor® 680

sc-25308 AF680
200 µg/ml
$357.00

Ep-CAM 抗体 (C-10) Alexa Fluor® 790

sc-25308 AF790
200 µg/ml
$357.00

For the representative images shown for each technique, could you please put the dilution amount used? Thanks.

Asked by: Oyku
Thank you for contacting Santa Cruz Biotechnology. Please see the datasheet for recommended dilutions .https://datasheets.scbt.com/sc-25308.pdf If you need further assistance please call (800)-457-3801 or email scbt@scbt.com to speak with our technical support department.
Answered by: BlakeJ
Date published: 2024-12-26

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Ep-CAM (C-10): sc-25308?

Asked by: jenni
We, recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems.
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 1 out of 5 by from immunofluoresecence stainingWe tested human cell line HepG2 and Panc1 in addition to human pancreatic cancer xenograft frozen tissue samples for immunofluorescence staining. However, the results were all negative...
Date published: 2019-12-16
Rated 1 out of 5 by from Very weak signal in WB (after IP)I used this antibody for Western Blot (1:200, 5% Milk/TBST) on a 2D WB membrane after EpCAM immunoprecipitation in HCT116 cells. I have observed very weak signal (as compared to other tested Anti-EpCAM antibodies). It definitely needs some sample-dependent optimization.
Date published: 2019-10-14
Rated 5 out of 5 by from Great!Tried anti-Ep-CAM antibody in immunofluorescence on cultured cells, very good staining, comparable with similar antibodies of different brands.
Date published: 2018-04-15
Rated 5 out of 5 by from 作为表面分子,更适合做免疫荧光epcam因为是表面分子,在我们的细胞株中表达并不是非常明显,所以wb表现一般,可能更加适合做流式或者免疫荧光
Date published: 2018-04-02
Rated 4 out of 5 by from GoodEn WB avec une dilution au 1/200, le signal est correcte.
Date published: 2018-01-16
Rated 1 out of 5 by from Bad results with Flow Citometry and Western BlotI received this as a free sample. I tried it with two different dilutions for FACS. First I tried with 1ul of antibody per 200000 cells and it didn´t work. Then I tried it with a dilution 2ul per 200000 cells and I neither got any good result. I also did Western Blot and I obteined a nonespecific signal in 20kDa (Molecular weight of EpCAM: 40kDa). For western blot I used a dilution 1:100.
Date published: 2017-10-12
Rated 4 out of 5 by from No problem with antibodiesIts intensity of the fluorescence image is a little weaker than cytokeratin and vimentin in A549 lung cells
Date published: 2017-06-22
Rated 5 out of 5 by from Great featureI got a sample from Santa Cruz, and used it to stain the epithelium via IHC. The images were very good.
Date published: 2017-06-13
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Ep-CAM 抗体 (C-10) is rated 4.1 out of 5 by 17.
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