Date published: 2025-9-7

021-6093-6350

SCBT Portrait Logo
Seach Input

eIF6 抗体 (A-2): sc-390432

4.7(7)
寫評論提問

说明书
  • eIF6 抗体 A-2 是小鼠单克隆 IgG2a κ,eIF6抗体, 在2篇文献中引用,规格为200 µg/ml
  • 免疫human物种的代表全长eIF6的氨基酸1-245
  • eIF6 抗体 (A-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 eIF6
  • 抗eIF6抗体(A-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取eIF6 (A-2): sc-390432免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 eIF6 抗体 (A-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与eIF6 抗体 (A-2) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

eIF6抗体(A-2)是一种IgG2a κ型的小鼠单克隆eIF6抗体(也称为eIF6抗体),能够通过WB、IP、IF、IHC(P)和ELISA等方法检测来自小鼠、大鼠和人类的eIF6蛋白。eIF6抗体(A-2)既有非偶联形式的抗eIF6抗体,也有多种偶联形式的抗eIF6抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。真核细胞中蛋白质合成的起始是由蛋白质起始因子和RNA分子之间的相互作用来调节的。真核起始因子(eIFs)被用于一系列反应中,这些反应导致80S核糖体组装,并最终导致翻译。eIF6(真核翻译起始因子6)也被称为CAB、B2GCN同源物、p27(BBP)或B4整合素相互作用物,是一种由245个氨基酸组成的蛋白质,定位于细胞质以及细胞核内的核仁。eIF6的N端和C端亚结构域被认为含有重要的核仁定位序列。eIF6可能是核糖体功能和生成的调节器。eIF6的功能是结合并将60S核糖体亚基从细胞核转移到细胞质,有效地阻止60S亚基与40S亚基结合,并抑制80S起始复合物的形成。80S核糖体的形成调控也调节转录。一旦转移到细胞质中,eIF6-60S核糖体亚基复合物就会通过RACK1/PKC途径进行磷酸化,这一事件导致eIF6从60S亚基上解离。eIF6的上调与多种癌症密切相关,如卵巢癌,这表明eIF6可能参与致癌过程。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

eIF6 抗体 (A-2) 参考文献:

  1. 核糖体抗结合因子 IF6 的晶体结构。  |  Groft, CM., et al. 2000. Nat Struct Biol. 7: 1156-64. PMID: 11101899
  2. 编码翻译引发剂 6 的酿酒酵母 TIF6 基因是 60S 核糖体亚基生物发生所必需的。  |  Basu, U., et al. 2001. Mol Cell Biol. 21: 1453-62. PMID: 11238882
  3. p27(BBP)/eIF6的磷酸化及其与细胞骨架的缔合作用在章鱼卵子发生过程中受发育调控。  |  Carotenuto, R., et al. 2005. Cell Mol Life Sci. 62: 1641-52. PMID: 15990955
  4. GABP 复合物可调控 eIF6(p27BBP)的转录,eIF6 是核糖体生物发生过程中必不可少的反式作用因子。  |  Donadini, A., et al. 2006. FEBS Lett. 580: 1983-7. PMID: 16530192
  5. 克隆竹荪 eIF6(p27BBP)并绘制其核(ol)定位亚域图。  |  Balbo, A. and Bozzaro, S. 2006. Eur J Cell Biol. 85: 1069-78. PMID: 16814427
  6. 真核细胞引发因子 6 选择性地调节 Wnt 信号传导和 beta-catenin 蛋白合成。  |  Ji, Y., et al. 2008. Oncogene. 27: 755-62. PMID: 17667944
  7. 卵巢浆液性癌患者的 eIF6 和 Dicer 表达改变与临床病理特征缔合作用。  |  Flavin, RJ., et al. 2008. Mod Pathol. 21: 676-84. PMID: 18327211
  8. 真核生物起始因子 6 在翻译, 生长和转化过程中起限速作用。  |  Gandin, V., et al. 2008. Nature. 455: 684-8. PMID: 18784653

订购信息

产品名称产品编号规格价格数量收藏夹

eIF6 抗体 (A-2)

sc-390432
200 µg/ml
$316.00

eIF6 (A-2): m-IgG Fc BP-HRP 套装

sc-530158
200 µg Ab; 10 µg BP
$354.00

eIF6 (A-2): m-IgGκ BP-HRP 套装

sc-523534
200 µg Ab, 40 µg BP
$354.00

eIF6 (A-2): m-IgG2a BP-HRP 套装

sc-547446
200 µg Ab; 10 µg BP
$354.00

eIF6 抗体 (A-2) AC

sc-390432 AC
500 µg/ml, 25% agarose
$416.00

eIF6 抗体 (A-2) HRP

sc-390432 HRP
200 µg/ml
$316.00

eIF6 抗体 (A-2) FITC

sc-390432 FITC
200 µg/ml
$330.00

eIF6 抗体 (A-2) PE

sc-390432 PE
200 µg/ml
$343.00

eIF6 抗体 (A-2) Alexa Fluor® 488

sc-390432 AF488
200 µg/ml
$357.00

eIF6 抗体 (A-2) Alexa Fluor® 546

sc-390432 AF546
200 µg/ml
$357.00

eIF6 抗体 (A-2) Alexa Fluor® 594

sc-390432 AF594
200 µg/ml
$357.00

eIF6 抗体 (A-2) Alexa Fluor® 647

sc-390432 AF647
200 µg/ml
$357.00

eIF6 抗体 (A-2) Alexa Fluor® 680

sc-390432 AF680
200 µg/ml
$357.00

eIF6 抗体 (A-2) Alexa Fluor® 790

sc-390432 AF790
200 µg/ml
$357.00

I am using eIF6 (A-2): sc-390432 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_390432, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 102ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Works super goodI bought this as a free sample and it works perfectly fine for western
Date published: 2022-09-02
Rated 4 out of 5 by from Finetested by WB on 293T WCE dilution 1/200, it seems to work fine, the signal is specific although a bit weak
Date published: 2017-12-14
Rated 5 out of 5 by from greatThis antibody works for mouse brain and intestine tissue immunostaining
Date published: 2017-09-06
Rated 5 out of 5 by from Beautiful immunostaing resultsGreat for co-localization studies. Good quality and specific antibody. Very satisfying results.
Date published: 2017-06-05
Rated 4 out of 5 by from Nucleolar and nuclear localization observedNucleolar and nuclear localization observed via immunofluorescence staining of formalin fixed HepG2 cells. -SCBT QC
Date published: 2015-05-16
Rated 5 out of 5 by from Cytoplasmic and nuclear staining observedCytoplasmic and nuclear staining observed of glandular and endothelial cells via immunoperoxidase staining of formalin fixed, paraffin embedded (FFPE) human colon tissue. -SCBT QC
Date published: 2015-01-28
Rated 5 out of 5 by from Bands observed at the expected molecularBands observed at the expected molecular weight observed via Western Blot using K-562, RKO, NIH/3T3, HEK293 and MIA PaCa-2 whole cell lysates. -SCBT QC
Date published: 2014-05-17
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_390432, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT
eIF6 抗体 (A-2) is rated 4.7 out of 5 by 7.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_390432, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 109ms
  • REVIEWS, PRODUCT