Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
EAAT4 Antibody (6D9) is a high quality monoclonal EAAT4 antibody (also designated EAAT4 antibody) suitable for the detection of the EAAT4 protein of mouse, rat and human origin. EAAT4 Antibody (6D9) is available as the non-conjugated anti-EAAT4 antibody. Excitatory Amino Acid Transporters (EAATs) are membrane-bound proteins that are localized in glial cells and pre-synaptic glutamatergic nerve endings. EAATs transport the excitatory neurotransmitters L-glutamate and D-aspartate, a process that is essential for terminating the postsynaptic action of glutamate. The re-uptake of amino acid neurotransmitters by EAAT proteins has been shown to protect neurons from excitotoxicity, which is caused by the accumulation of amino acid neurotransmitters. EAAT4 is an aspartate/glutamate transporter that is expressed predominantly in the cerebellum. The transport activity encoded by EAAT4 has high apparent affinity for L-aspartate and L-glutamate, and has a pharmacologic profile consistent with previously described cerebellar transport activities. EAAT5 is a glutamate transporter coupled to a chloride conductance which is expressed primarily in retina. Although EAAT5 shares the structural homologies of the EAAT family, a novel feature of the EAAT5 sequence is a carboxy-terminal motif previously identified in N-ethyl-D-aspartate receptors and potassium channels and shown to confer interactions with a family of synaptic proteins that promote ion channel clustering.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Hi I tried this antibody sc-293344 (1:500 dilution) and got no signal in my cells (they express EAAT4 in qPCR). What is the size of this protein because in your photo it looks like its around 35kDa but I found in the literature it should be 62kDa. Thanks.
Asked by: smirkov
Thank you for your question. The expected molecular weight for EAAT4 is approximately 67 kDa. The Western blot data you are referring to on our website is not representative of the endogenous molecular weight for EAAT4 because it using a recombinant fusion protein (not full length).
Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-04-11
For Western Blot, is it recommended to use denatured or non-denatured conditions with EAAT4 (6D9): sc-293344 antibody?
Asked by: jerojero
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.