EAAT4抗体 (6D9) 推荐用于 mouse, rat 和 human 来源的EAAT4 WB, IP 和 ELISA检测

EAAT4 抗体 (6D9): sc-293344

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说明书

    • 抗-EAAT4 抗体 6D9 是小鼠单克隆 IgG1 κ,EAAT4抗体, 在1篇文献中引用,规格为100 µg/ml
    • 免疫human物种的EAAT4的氨基酸500-564
    • 推荐用于 mouse, rat 和 human 来源的EAAT4 WB, IP 和 ELISA检测

Hi I tried this antibody sc-293344 (1:500 dilution) and got no signal in my cells (they express EAAT4 in qPCR). What is the size of this protein because in your photo it looks like its around 35kDa but I found in the literature it should be 62kDa. Thanks.

Asked by: smirkov
Thank you for your question. The expected molecular weight for EAAT4 is approximately 67 kDa. The Western blot data you are referring to on our website is not representative of the endogenous molecular weight for EAAT4 because it using a recombinant fusion protein (not full length). Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-04-11

For Western Blot, is it recommended to use denatured or non-denatured conditions with EAAT4 (6D9): sc-293344 antibody?

Asked by: jerojero
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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