Date published: 2025-9-6
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E2F-3 Antibody (PG30) - Western Blotting - Image 354813
E2F-3 抗体 (PG30): sc-56665. COLO 320DM (A), Jurkat (B), SK-N-SH (C), SH-SY5Y (D) 和 HEL 92. 1. 7 (E) 全细胞裂解液中 E2F-3 表达的 Western 印迹分析.
E2F-3 抗体 (PG30): sc-56665. COLO 320DM (A), Jurkat (B), SK-N-SH (C), SH-SY5Y (D) 和 HEL 92. 1. 7 (E) 全细胞裂解液中 E2F-3 表达的 Western 印迹分析.

E2F-3 抗体 (PG30): sc-56665

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说明书
  • E2F-3 抗体 PG30 是小鼠单克隆 IgG2a κ，E2F-3抗体, 在15篇文献中引用，规格为200 µg/ml
  • 免疫human物种的重组E2F-3
  • E2F-3 抗体 (PG30) 推荐用于 WB 和 IP，检测mouse, rat 和human 来源的 E2F-3; 不会与 other members of the E2F family产生交联反应
  • 抗E2F-3抗体（PG30）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取E2F-3 (PG30): sc-56665的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是E2F-3 Antibody (PG30) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与E2F-3 Antibody (PG30) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

附加E2F抗体，包括E2F-1, E2F-2, E2F-3, E2F-4, E2F-5, E2F-6和E2F-8

E2F-3抗体（PG30）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）和免疫沉淀（IP）检测小鼠、大鼠和人类来源的E2F-3蛋白。抗E2F-3抗体 （PG30）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。E2F-3蛋白在调节细胞周期中发挥着关键作用，特别是在DNA合成发生的G1期到S期的过渡期。这种调节对于细胞的正常增殖和分化至关重要，因为E2F-3的失调会导致细胞无序生长，并常常与癌症发展有关。E2F-3是一种转录因子，可激活细胞周期进展所需的基因，其活性与视网膜母细胞瘤（Rb）蛋白的相互作用密切相关。Rb蛋白在发挥作用时，会与E2F-3结合并抑制转录活性，从而防止过早进入S期。了解E2F-3的动态和调节机制对于制定针对各种癌症细胞周期失调的治疗策略至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

E2F-3 抗体 (PG30) 参考文献:

  1. 编码具有转录因子 E2F 特性的 pRB 结合蛋白的 cDNA。  |  Helin, K., et al. 1992. Cell. 70: 337-50. PMID: 1638634
  2. E2F 转录因子是 RB 蛋白的细胞靶标。  |  Chellappan, SP., et al. 1991. Cell. 65: 1053-61. PMID: 1828392
  3. 视网膜母细胞瘤产物的 T/E1A 结合域可选择性地与序列特异性 DNA 结合蛋白相互作用。  |  Chittenden, T., et al. 1991. Cell. 65: 1073-82. PMID: 1828394
  4. lncRNA NEAT1/miRNA-766-5p/E2F3调控轴促进前列腺癌进展  |  Zhao, W., et al. 2022. J Oncol. 2022: 1866972. PMID: 35237319
  5. Circ_001422通过靶向miR-497-5p/E2F3轴加剧骨肉瘤的进展。  |  Li, X., et al. 2023. J Biochem Mol Toxicol. 37: e23392. PMID: 37287369
  6. E2F3 通过激活 STAT3 通路加速结肠癌细胞的干性。  |  Gao, Q., et al. 2023. Front Oncol. 13: 1203712. PMID: 37456248
  7. E2F-4是E2F转录因子家族的新成员，与p107相互作用。  |  Ginsberg, D., et al. 1994. Genes Dev. 8: 2665-79. PMID: 7958924
  8. E2F-4是E2F基因家族的新成员，具有致癌活性，并在体内与p107结合。  |  Beijersbergen, RL., et al. 1994. Genes Dev. 8: 2680-90. PMID: 7958925
  9. 与 DNA 和视网膜母细胞瘤基因产物的结合由不同 E2F 家族成员形成的复合物促进。  |  Krek, W., et al. 1993. Science. 262: 1557-60. PMID: 8248803
  10. 转录因子 E2F-1 和 DP-1 的异源二聚化导致了合作性的转激活。  |  Helin, K., et al. 1993. Genes Dev. 7: 1850-61. PMID: 8405995
  11. E2F-1 和 E2F-3 在促进髓细胞周期进展和阻断粒细胞分化方面的功能不同。  |  Strom, DK., et al. 1998. Cell Growth Differ. 9: 59-69. PMID: 9438389
  12. E2F-6 是 E2F 家族的成员，可作为转录抑制因子。  |  Trimarchi, JM., et al. 1998. Proc Natl Acad Sci U S A. 95: 2850-5. PMID: 9501179

订购信息

产品名称产品编号规格价格数量收藏夹

E2F-3 抗体 (PG30)

sc-56665
200 µg/ml
$316.00

E2F-3 (PG30): m-IgG Fc BP-HRP 套装

sc-528617
200 µg Ab; 10 µg BP
$354.00

E2F-3 (PG30): m-IgGκ BP-HRP 套装

sc-521040
200 µg Ab, 40 µg BP
$354.00

E2F-3 (PG30): m-IgG2a BP-HRP 套装

sc-547130
200 µg Ab; 10 µg BP
$354.00

E2F-3 抗体 (PG30) AC

sc-56665 AC
500 µg/ml, 25% agarose
$416.00

E2F-3 抗体 (PG30) HRP

sc-56665 HRP
200 µg/ml
$316.00

E2F-3 抗体 (PG30) FITC

sc-56665 FITC
200 µg/ml
$330.00

E2F-3 抗体 (PG30) PE

sc-56665 PE
200 µg/ml
$343.00

E2F-3 抗体 (PG30) Alexa Fluor® 488

sc-56665 AF488
200 µg/ml
$357.00

E2F-3 抗体 (PG30) Alexa Fluor® 546

sc-56665 AF546
200 µg/ml
$357.00

E2F-3 抗体 (PG30) Alexa Fluor® 594

sc-56665 AF594
200 µg/ml
$357.00

E2F-3 抗体 (PG30) Alexa Fluor® 647

sc-56665 AF647
200 µg/ml
$357.00

E2F-3 抗体 (PG30) Alexa Fluor® 680

sc-56665 AF680
200 µg/ml
$357.00

E2F-3 抗体 (PG30) Alexa Fluor® 790

sc-56665 AF790
200 µg/ml
$357.00

Does this antibody differentiate between E2F3a and E2F3b?

Asked by: Malvika
Thank you for your question. For assistance please contact Technical support. Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-07-23

Is the antibodies epitope C-terminal or N-terminal? Can it differentiate between E2F3A and E2F3B?

Asked by: WWeston
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2024-01-25

Kann dieser Antikörper für immunhistochemische Analysen (FFPE-Material) an menschlichem Tumorgewebe verwendet werden? Wenn ja, in welcher Konzentration?

Asked by: Annette20
Vielen Dank für Ihre Anfrage. Anti-E2F-3 Antikörper (PG30) ist empfohlen für die Detektion von E2F-3 aus Maus, Ratte und Mensch per WB und IP. Basierend auf unseren Tests mit dem Antikörper ist er nicht für IHC(P) Anwendungen geeignet. Leider ist keiner der EF2F-3 Antikörper in unserem derzeitigen Portfolio dafür empfohlen. Thank you for your question. Unfortunately, this antibody is not recommended for IHC(P) applications for human tumor tissues. We currently do not offer any antibody that would be suitable for such a use.
Answered by: Tech Support Europe
Date published: 2020-11-02

Are there any issues with using sc-56665: E2F-3 (PG30) mouse monoclonal primary antibody on mouse samples?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-56665 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 1 out of 5 by from not workingnot working. I bought this Ab instead of discontined SC-879, because it was recommended.
Date published: 2019-06-20
Rated 5 out of 5 by from It works for IFWe tested it for IF with wild type and E2F3 knockout HAP1 cells and detected specific signals in nucleus, whereas the signals were vanished in KO cells.
Date published: 2018-09-20
Rated 1 out of 5 by from Didnot work for WBdid not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 4 out of 5 by from Ottimo funzionamentoBuona sensibilità e specificità dell'Ab alle condizioni suggerite dal datasheet
Date published: 2017-11-20
Rated 5 out of 5 by from The Product is GoodE2F-3 antibody is being used for blotting. The analysis results of Western Blot were very strong and clear, the express high yield.
Date published: 2017-10-16
Rated 5 out of 5 by from specific bandWe use the positive control SK-N-MC cell lysate for analysis E2F-3 expression, it verified that this antibody is very perfect at dilution 1:800,no non-specific band and satisfied specific band.
Date published: 2017-08-26
Rated 5 out of 5 by from solid choiceGood antibody in WB using human breast cancer cells, the antibody gave me a good result.
Date published: 2017-02-12
Rated 5 out of 5 by from Excellent!This antibody is great in western blot analysis of E2F-3 expression in K-562 and Y79 whole cell lysates and K-562), Y79 (C) and PMA treated Y79 (E) nuclear extracts.
Date published: 2017-02-02
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E2F-3 抗体 (PG30) is rated 4.1 out of 5 by 11.
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