Date published: 2025-9-6
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E2A Antibody (G-2) - Western Blotting - Image 361115
E2A 抗体 (G-2): sc-133075. Raji (A) 和 Ramos (B) 全细胞裂解液中 E2A 表达的 Western 印迹分析.
E2A 抗体 (G-2): sc-133075. Raji (A) 和 Ramos (B) 全细胞裂解液中 E2A 表达的 Western 印迹分析.

E2A 抗体 (G-2): sc-133075

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寫評論提問

说明书
  • E2A 抗体 G-2 是小鼠单克隆 IgG1 κ，E2A抗体, 在8篇文献中引用，规格为200 µg/ml
  • 免疫human物种的E2A的氨基酸1-649
  • E2A 抗体 (G-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 E2A isoforms E12 and E47
  • 抗E2A抗体（G-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-133075 X, 200 µg/0.1 ml)
  • 关于如何获取E2A (G-2): sc-133075的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 E2A 抗体 (G-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与E2A 抗体 (G-2) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

E抗体

E2A抗体（G-2）是一种IgG1κ小鼠单克隆E2A抗体（也称为转录因子3（TCF-3）抗体、ITF1抗体、ITF-1抗体、E蛋白（I类）螺旋-环-螺旋转录因子抗体、E2A免疫球蛋白增强子结合因子E12/E47抗体、p75抗体、碱性螺旋-环-螺旋（bHLHb21）二聚化转录调节因子抗体、调节E盒启动子序列基序结合二聚化转录因子抗体或E盒/同源盒（约180碱基对）结合核转录因子抗体），可通过WB、IP、IF、IHC（P）和ELISA检测人源E2A蛋白。 E2A抗体（G-2）既可作为非偶联抗E2A抗体形式，也可作为抗E2A抗体的多种偶联形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。转录因子3（E47、E12、E2A免疫球蛋白增强子结合因子E12/E47、E2A、ITF1、TCF3）在B细胞成熟过程中影响基因表达。肌源性细胞的分化受多种正负作用因子的调节。已知在肌肉细胞发育的调节中起重要作用的螺旋-环-螺旋（HLH）蛋白家族，包括Myo D、肌细胞生成素、Myf-5和herculin。 Myo D转录因子与bHLH基因家族中表达更广泛的产物形成异二聚体，bHLH基因家族由至少三个不同的基因组成：E2A、IF2和HEB。 Myo D-E异二聚体与共有序列（CANNTG）E盒靶位点高度结合，这些靶位点是许多肌肉特异性终末分化基因上游调控序列中的重要元件。这些蛋白质的同源和异源寡聚体能够区分非常相近的E盒蛋白质，并被认为在谱系特异性基因表达中起重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

E2A 抗体 (G-2) 参考文献:

  1. E2A 家族蛋白对早期淋巴细胞发育的调控。  |  Greenbaum, S. and Zhuang, Y. 2002. Semin Immunol. 14: 405-14. PMID: 12457613
  2. E2A 蛋白：正常生理和疾病中细胞表型的调节器。  |  Slattery, C., et al. 2008. Int J Biochem Cell Biol. 40: 1431-6. PMID: 17604208
  3. E2A-核心抑制因子相互作用在不同致白血病途径中的不同参与程度。  |  Gow, CH., et al. 2014. Nucleic Acids Res. 42: 137-52. PMID: 24064250
  4. TCF3/E2A 的抑制有助于霍奇金淋巴瘤的形成。  |  Guan, H., et al. 2016. Oncotarget. 7: 36854-36864. PMID: 27166193
  5. E2A 通过 Wnt/beta-catenin 通路削弱结直肠癌细胞的肿瘤诱发能力。  |  Zhao, H., et al. 2019. J Exp Clin Cancer Res. 38: 276. PMID: 31234887
  6. 转录因子E2A驱动多能细胞的神经分化。  |  Rao, C., et al. 2020. Development. 147: PMID: 32487737
  7. 介导亚基 MED1 是 E2A-PBX1 介导的致癌转录物和白血病细胞生长所必需的。  |  Lee, YL., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33542097
  8. E2A调控的表观遗传景观促进了记忆性CD8 T细胞的分化。  |  Schauder, DM., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33859041
  9. E2A 在 ATPR 诱导的急性髓性白血病细胞分化和周期停滞中的作用。  |  Zhang, M., et al. 2022. J Cell Mol Med. 26: 1128-1143. PMID: 35001521
  10. 转录因子 E2A 可与单链免疫球蛋白重链基因座 DNA 结合并裂解。  |  Grundström, C. and Grundström, T. 2023. Mol Immunol. 153: 51-59. PMID: 36434987

订购信息

产品名称产品编号规格价格数量收藏夹

E2A 抗体 (G-2)

sc-133075
200 µg/ml
$316.00

E2A (G-2): m-IgG Fc BP-HRP 套装

sc-528740
200 µg Ab; 10 µg BP
$354.00

E2A (G-2): m-IgGκ BP-HRP 套装

sc-521223
200 µg Ab, 40 µg BP
$354.00

E2A (G-2): m-IgG1 BP-HRP 套装

sc-543106
200 µg Ab; 20 µg BP
$354.00

E2A 抗体 (G-2) X

sc-133075 X
200 µg/0.1 ml
$316.00

E2A 抗体 (G-2) AC

sc-133075 AC
500 µg/ml, 25% agarose
$416.00

E2A 抗体 (G-2) HRP

sc-133075 HRP
200 µg/ml
$316.00

E2A 抗体 (G-2) FITC

sc-133075 FITC
200 µg/ml
$330.00

E2A 抗体 (G-2) PE

sc-133075 PE
200 µg/ml
$343.00

E2A 抗体 (G-2) Alexa Fluor® 488

sc-133075 AF488
200 µg/ml
$357.00

E2A 抗体 (G-2) Alexa Fluor® 546

sc-133075 AF546
200 µg/ml
$357.00

E2A 抗体 (G-2) Alexa Fluor® 594

sc-133075 AF594
200 µg/ml
$357.00

E2A 抗体 (G-2) Alexa Fluor® 647

sc-133075 AF647
200 µg/ml
$357.00

E2A 抗体 (G-2) Alexa Fluor® 680

sc-133075 AF680
200 µg/ml
$357.00

E2A 抗体 (G-2) Alexa Fluor® 790

sc-133075 AF790
200 µg/ml
$357.00

What do you recommend as a blocking reagent: BSA or Milk?

Asked by: Researcher
Thank you for your question. This antibody has been established with UltraCruz® Blocking Reagent: sc-516214, which we recommend for blocking purposes.
Answered by: Tech Support Europe
Date published: 2025-05-09

Is it tested in B cells?

Asked by: Researcher
Thank you for your question. E2A Antibody (G-2): sc-133075 has been tested on a wide panel of different samples and is recommended for detection of E2A isoforms E12 and E47 of human origin by WB, IP, IF, IHC(P) and ELISA. It should be compatible with B cells of human origin as well.
Answered by: Tech Support Europe
Date published: 2025-04-30

When should the TransCruz reagent sc-133075 X be used instead of the standard form sc-133075?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2018-05-03
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Rated 1 out of 5 by from Bad for IFWe used this antibody in immunostaining. But it didn't work well.
Date published: 2021-12-07
Rated 5 out of 5 by from good resultI'm very glad to use this antibody,it gave us good results with for IF (1:200) and WB(1:800).
Date published: 2017-09-02
Rated 5 out of 5 by from high qualityThe antibody conceration is high enough when used 1:1000, WB detection of E2A protein shows strong band and no background and non-specific bands.I recommend to my labmates.
Date published: 2017-08-26
Rated 5 out of 5 by from clear bandsGet clear bands at correct position in my samples.And the molecular weight is right. And IF work well too
Date published: 2017-04-15
Rated 5 out of 5 by from strong bandUsed at 1:500 to detect E2A,works good,single specific band detected,staining data very strong.
Date published: 2017-02-26
Rated 5 out of 5 by from Good antibodyE2A antibody provides clear and specific results and has been cited many times.
Date published: 2017-02-04
Rated 5 out of 5 by from Good WB resultWe had to test different dilutions in WB but we finally got our results with 1:300 shows clera band .
Date published: 2017-02-02
Rated 5 out of 5 by from Produced excellent immunofluorescence nuclearProduced excellent immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-05-18
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E2A 抗体 (G-2) is rated 4.6 out of 5 by 9.
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