Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Dyskerin Antibody (H-3) - Western Blotting - Image 362884
Dyskerin 抗体 (H-3): sc-373956. HeLa 核提取物 (A) 和 MCF7 (B) 及 A-431 (C) 全细胞裂解液中 Dyskerin 表达的 Western 印迹分析.
Dyskerin 抗体 (H-3): sc-373956. HeLa 核提取物 (A) 和 MCF7 (B) 及 A-431 (C) 全细胞裂解液中 Dyskerin 表达的 Western 印迹分析.

Dyskerin 抗体 (H-3): sc-373956

4.5(8)
寫評論提問

说明书
  • Dyskerin 抗体 H-3 是小鼠单克隆 IgG2a κ，Dyskerin抗体, 在27篇文献中引用，规格为200 µg/ml
  • 针对human起源的Dyskerin内部区域内的氨基酸171-470进行
  • Dyskerin 抗体 (H-3) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Dyskerin
  • 抗Dyskerin抗体（H-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-373956 X, 200 µg/0.1 ml)
  • 关于如何获取Dyskerin (H-3): sc-373956的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是Dyskerin Antibody (H-3) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Dyskerin Antibody (H-3) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Dyskerin抗体（H-3）是一种IgG2a κ小鼠单克隆Dyskerin抗体（也称为DKC1抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人的Dyskerin蛋白。Dyskerin抗体（H-3）既可以是非偶联的抗Dyskerin抗体形式，也可以是多种偶联形式的抗Dyskerin抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Dyskerin（NAP57）与伴侣蛋白Nopp140结合，并与GAR1（NOLA1）、NHP2（NOLA2）和Nop10形成小核糖核蛋白颗粒，用于将尿苷异构化为假尿苷。GAR1、NHP2和dyskerin定位于核仁的致密纤维组分和核Cajal体中。Dyskerin基因定位于Xq28染色体。Dyskerin基因中的错义突变会干扰dyskerin的正常核定位，并导致先天性角化不良（DKC）。DKC是一种罕见的X连锁骨髓疾病，其特征是皮肤色素沉着过度、指甲营养不良、睾丸萎缩和口腔黏膜白斑。GAR1基因定位于4q25染色体。NHP2基因定位于5q35.3染色体，并编码一个由155个氨基酸组成的蛋白质。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Dyskerin 抗体 (H-3) 参考文献:

  1. Dyskerin 定位于核仁，其错误定位不太可能在先天性角化异常的发病机制中发挥作用。  |  Heiss, NS., et al. 1999. Hum Mol Genet. 8: 2515-24. PMID: 10556300
  2. 人类 H/ACA 小核极 RNPs 的体外组装揭示了 U17 和端粒酶 RNA 的独特特征。  |  Dragon, F., et al. 2000. Mol Cell Biol. 20: 3037-48. PMID: 10757788
  3. 人类 H/ACA 小核仁 RNPs 和端粒酶共享进化保守蛋白 NHP2 和 NOP10。  |  Pogacić, V., et al. 2000. Mol Cell Biol. 20: 9028-40. PMID: 11074001
  4. 免疫纯化的核小体核糖核蛋白颗粒与磷酸化的 Nopp140 的结合无关，是 rRNA 的假核苷酸化。  |  Wang, C., et al. 2002. Mol Cell Biol. 22: 8457-66. PMID: 12446766
  5. 在X连锁先天性角化不全症和端粒酶缺陷的人体细胞中，dyskerin基因启动子可重新激活端粒酶活性。  |  Machado-Pinilla, R., et al. 2008. Blood. 111: 2606-14. PMID: 18057229
  6. 通过缺陷的mRNA翻译，Dyskerin介导的p53失活的新机制。  |  Montanaro, L., et al. 2010. Cancer Res. 70: 4767-77. PMID: 20501855
  7. Dyskerin和癌症：不仅仅是端粒酶。mRNA翻译缺陷有助于解释增殖缺陷如何导致癌症。  |  Montanaro, L. 2010. J Pathol. 222: 345-9. PMID: 20925138
  8. 一种新的细胞质定位的人源性dyskerin同种型。  |  Angrisani, A., et al. 2011. Biochim Biophys Acta. 1810: 1361-8. PMID: 21820037
  9. 对dyskerin在肺癌细胞中的作用进行多尺度原位分析。  |  Fernandez-Garcia, I., et al. 2013. Integr Biol (Camb). 5: 402-13. PMID: 23233094
  10. X连锁先天性角化不全（DKCX）的严重程度与核糖体RNA生物合成或mRNA翻译中的dyskerin（DKC1）活性没有直接关系。  |  Thumati, NR., et al. 2013. Hum Mutat. 34: 1698-707. PMID: 24115260
  11. 通过调节 dyskerin 的表达发现新的色烯-4-酮衍生物作为端粒酶抑制剂。  |  Quan Wang, J., et al. 2018. J Enzyme Inhib Med Chem. 33: 1199-1211. PMID: 30132373
  12. X 连锁先天性角化异常（DKC）基因的图谱绘制和特征描述。  |  Hassock, S., et al. 1999. Genomics. 55: 21-7. PMID: 9888995

订购信息

产品名称产品编号规格价格数量收藏夹

Dyskerin 抗体 (H-3)

sc-373956
200 µg/ml
$316.00

Dyskerin (H-3): m-IgG Fc BP-HRP 套装

sc-529620
200 µg Ab; 10 µg BP
$354.00

Dyskerin (H-3): m-IgGκ BP-HRP 套装

sc-522569
200 µg Ab, 40 µg BP
$354.00

Dyskerin (H-3): m-IgG2a BP-HRP 套装

sc-547342
200 µg Ab; 10 µg BP
$354.00

Dyskerin 抗体 (H-3) X

sc-373956 X
200 µg/0.1 ml
$316.00

Dyskerin 抗体 (H-3) AC

sc-373956 AC
500 µg/ml, 25% agarose
$416.00

Dyskerin 抗体 (H-3) HRP

sc-373956 HRP
200 µg/ml
$316.00

Dyskerin 抗体 (H-3) FITC

sc-373956 FITC
200 µg/ml
$330.00

Dyskerin 抗体 (H-3) PE

sc-373956 PE
200 µg/ml
$343.00

Dyskerin 抗体 (H-3) Alexa Fluor® 488

sc-373956 AF488
200 µg/ml
$357.00

Dyskerin 抗体 (H-3) Alexa Fluor® 546

sc-373956 AF546
200 µg/ml
$357.00

Dyskerin 抗体 (H-3) Alexa Fluor® 594

sc-373956 AF594
200 µg/ml
$357.00

Dyskerin 抗体 (H-3) Alexa Fluor® 647

sc-373956 AF647
200 µg/ml
$357.00

Dyskerin 抗体 (H-3) Alexa Fluor® 680

sc-373956 AF680
200 µg/ml
$357.00

Dyskerin 抗体 (H-3) Alexa Fluor® 790

sc-373956 AF790
200 µg/ml
$357.00

I have done WB using 1:100 dilution in 5% non-fat milk and I can not see any band. Any recommendations

Asked by: AyaA
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-10-27

We would like to test your Dyskerin Antibody (H-3): sc-373956 in our research in Arabidopsis plants, could you please send me a free sample

Asked by: jsaez
Thank you for the question. Our Technical Service Dept. will contact you shortly.
Answered by: Technical Support Europe
Date published: 2018-01-19

What is the recommended fixation method for immunofluorescence staining with Dyskerin (H-3): sc-373956 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_373956, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 104ms
  • QUESTIONS, PRODUCT
Rated 3 out of 5 by from ChIP at telomeresThis antibody worked well for telomeric ChIP in 293T cells.
Date published: 2018-02-26
Rated 5 out of 5 by from Works for WB/IF/PLABought this antibody (DKC1) to measure protein-protein interactions (amongst other things) in 293T/HeLa lysates. Worked great! WB 1:1500 and IF/PLA 1:250. Also used alongside irradiated samples and worked like a charm.
Date published: 2017-08-15
Rated 5 out of 5 by from nice antibodycan recognize dyskerin in different lung cancer cells
Date published: 2017-04-04
Rated 5 out of 5 by from Excellent choice for Dyskerin!This antibody gives beautiful bands on Western blots, especially when used with the Mouse IgG binding protein reagent! Strong and reliable, it's a win-win!
Date published: 2017-01-18
Rated 4 out of 5 by from Great for IFWorks well for indirect immunofluorescence on PFA fixed HeLa cells, but was unsuccessful on westerns of whole cell extracts of the same cells.
Date published: 2016-12-27
Rated 4 out of 5 by from Robust band seen in WB using HeLa nuclear extractRobust band seen in WB using HeLa nuclear extract. -SCBT QC
Date published: 2015-05-24
Rated 5 out of 5 by from Beautiful immunofluorescence nucleolar stainingBeautiful immunofluorescence nucleolar staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-20
Rated 5 out of 5 by from Strong band detected in WB using Raji wholeStrong band detected in WB using Raji whole cell lysate. -SCBT QC
Date published: 2015-04-11
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_373956, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 13ms
  • REVIEWS, PRODUCT
Dyskerin 抗体 (H-3) is rated 4.5 out of 5 by 8.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_373956, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 100ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。