Dyskerin抗体 (H-3) 推荐用于 mouse, rat 和 human 来源的Dyskerin WB, IP, IF 和 ELISA检测

Dyskerin 抗体 (H-3): sc-373956

Dyskerin抗体 (H-3) is rated 4.5 out of 5 by 8.
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说明书

    • 抗-Dyskerin 抗体 H-3 是小鼠单克隆 IgG2a κ,Dyskerin抗体, 在8篇文献中引用,规格为200 µg/ml
    • raised against amino acids 171-470 mapping within an internal region of Dyskerin of human origin
    • 抗-Dyskerin 抗体 (H-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Dyskerin
    • 抗-Dyskerin 抗体 (H-3) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 可用于ChIP的TransCruz试剂(sc-373956 X, 200 µg/0.1 ml)
    • 关于如何获取Dyskerin (H-3): sc-373956免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Dyskerin Antibody (H-3) for WB applications. This reagent is now offered in a bundle with Dyskerin Antibody (H-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

We would like to test your Dyskerin Antibody (H-3): sc-373956 in our research in Arabidopsis plants, could you please send me a free sample

Asked by: jsaez
Thank you for the question. Our Technical Service Dept. will contact you shortly.
Answered by: Technical Support Europe
Date published: 2018-01-19

What is the recommended fixation method for immunofluorescence staining with Dyskerin (H-3): sc-373956 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from ChIP at telomeres This antibody worked well for telomeric ChIP in 293T cells.
Date published: 2018-02-26
Rated 5 out of 5 by from Works for WB/IF/PLA Bought this antibody (DKC1) to measure protein-protein interactions (amongst other things) in 293T/HeLa lysates. Worked great! WB 1:1500 and IF/PLA 1:250. Also used alongside irradiated samples and worked like a charm.
Date published: 2017-08-15
Rated 5 out of 5 by from nice antibody can recognize dyskerin in different lung cancer cells
Date published: 2017-04-04
Rated 5 out of 5 by from Excellent choice for Dyskerin! This antibody gives beautiful bands on Western blots, especially when used with the Mouse IgG binding protein reagent! Strong and reliable, it's a win-win!
Date published: 2017-01-18
Rated 4 out of 5 by from Great for IF Works well for indirect immunofluorescence on PFA fixed HeLa cells, but was unsuccessful on westerns of whole cell extracts of the same cells.
Date published: 2016-12-27
Rated 4 out of 5 by from Robust band seen in WB using HeLa nuclear extract Robust band seen in WB using HeLa nuclear extract. -SCBT QC
Date published: 2015-05-24
Rated 5 out of 5 by from Beautiful immunofluorescence nucleolar staining Beautiful immunofluorescence nucleolar staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-20
Rated 5 out of 5 by from Strong band detected in WB using Raji whole Strong band detected in WB using Raji whole cell lysate. -SCBT QC
Date published: 2015-04-11
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