Date published: 2025-9-7

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Dynactin p62 抗体 (H-4): sc-55603

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说明书
  • Dynactin p62 抗体 H-4 是小鼠单克隆 IgG2a κ,Dynactin p62抗体, 在9篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Dynactin p62的氨基酸161-460
  • Dynactin p62 抗体 (H-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Dynactin p62
  • 抗Dynactin p62抗体(H-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Dynactin p62 (H-4): sc-55603免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 Dynactin p62 抗体 (H-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Dynactin p62 抗体 (H-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Dynactin p62抗体(H-4)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的Dynactin p62 )。抗Dynactin p62抗体(H-4)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Dynactin p62在由微管马达驱动的细胞过程中起着至关重要的作用,是这些机制中重要的辅助因子。Dynactin p62是dynactin复合物的重要组成部分,对于dynein的正常功能至关重要,可促进细胞内运输和细胞器运动。Dynactin p62包含一个高度保守的富含半胱氨酸的基序,可与肌动蛋白相关蛋白1(Arp1)直接相互作用,从而促进dynactin复合物的结构完整性和功能性。Dynactin p62呈点状分布于细胞质中,定位于中心体,在细胞核中的表达水平也很高。这种分布模式表明其在细胞动力学中具有多方面的作用,将动力蛋白和动力蛋白复合物与细胞质骨架联系起来,并影响各种细胞过程,包括有丝分裂和细胞器定位。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Dynactin p62 抗体 (H-4) 参考文献:

  1. 肌动蛋白相关蛋白 Arp1 的自我调节聚合。  |  Bingham, JB. and Schroer, TA. 1999. Curr Biol. 9: 223-6. PMID: 10074429
  2. 对动态蛋白亚复合物的分析揭示了一种与 arp1 小丝尖端缔合作用的新型肌动蛋白相关蛋白。  |  Eckley, DM., et al. 1999. J Cell Biol. 147: 307-20. PMID: 10525537
  3. 动态蛋白 p62 亚基与 Arp1 和皮层肌动蛋白细胞骨架的相互作用  |  Garces, JA., et al. Curr Biol. 9: 1497-500. PMID: 10607597
  4. 具有高度保守的富半胱氨酸基团的动态蛋白亚基与 Arp1 直接相互作用。  |  Karki, S., et al. 2000. J Biol Chem. 275: 4834-9. PMID: 10671518
  5. 培养的巨噬细胞中细胞质动力蛋白和动力蛋白亚基的免疫定位:富集于早期内吞细胞器。  |  Habermann, A., et al. 2001. J Cell Sci. 114: 229-240. PMID: 11112706
  6. 依赖铜的动态蛋白亚基 p62 与 ATP7B N 端而非 ATP7A 的相互作用  |  Lim, CM., et al. 2006. J Biol Chem. 281: 14006-14. PMID: 16554302
  7. 动力蛋白复合物的p25亚基是动力蛋白-早期内体相互作用所必需的。  |  Zhang, J., et al. 2011. J Cell Biol. 193: 1245-55. PMID: 21708978
  8. Dynactin通过其左旋β螺旋p27亚基帮助Polo样激酶1定位到动点。  |  Yeh, TY., et al. 2013. EMBO J. 32: 1023-35. PMID: 23455152
  9. 冷冻电镜揭示了动态蛋白肩区和尖端复杂的结构  |  Lau, CK., et al. 2021. EMBO J. 40: e106164. PMID: 33734450
  10. 海马神经元中甲基苯丙胺诱导的自噬体积累之谜:在动力蛋白-动力蛋白驱动和自噬体-晚期内膜体/溶酶体融合缺陷中失去句法蛋白17。  |  Xu, H., et al. 2021. Arch Toxicol. 95: 3263-3284. PMID: 34374793
  11. 动态蛋白复合体的超微结构分析:一种肌动蛋白相关蛋白是类似于 F-肌动蛋白的丝的组成部分。  |  Schafer, DA., et al. 1994. J Cell Biol. 126: 403-12. PMID: 7518465

订购信息

产品名称产品编号规格价格数量收藏夹

Dynactin p62 抗体 (H-4)

sc-55603
200 µg/ml
$316.00

Dynactin p62 (H-4): m-IgG Fc BP-HRP 套装

sc-528611
200 µg Ab; 10 µg BP
$354.00

Dynactin p62 (H-4): m-IgGκ BP-HRP 套装

sc-521031
200 µg Ab, 40 µg BP
$354.00

Dynactin p62 (H-4): m-IgG2a BP-HRP 套装

sc-547129
200 µg Ab; 10 µg BP
$354.00

Dynactin p62 抗体 (H-4) AC

sc-55603 AC
500 µg/ml, 25% agarose
$416.00

Dynactin p62 抗体 (H-4) HRP

sc-55603 HRP
200 µg/ml
$316.00

Dynactin p62 抗体 (H-4) FITC

sc-55603 FITC
200 µg/ml
$330.00

Dynactin p62 抗体 (H-4) PE

sc-55603 PE
200 µg/ml
$343.00

Dynactin p62 抗体 (H-4) Alexa Fluor® 488

sc-55603 AF488
200 µg/ml
$357.00

Dynactin p62 抗体 (H-4) Alexa Fluor® 546

sc-55603 AF546
200 µg/ml
$357.00

Dynactin p62 抗体 (H-4) Alexa Fluor® 594

sc-55603 AF594
200 µg/ml
$357.00

Dynactin p62 抗体 (H-4) Alexa Fluor® 647

sc-55603 AF647
200 µg/ml
$357.00

Dynactin p62 抗体 (H-4) Alexa Fluor® 680

sc-55603 AF680
200 µg/ml
$357.00

Dynactin p62 抗体 (H-4) Alexa Fluor® 790

sc-55603 AF790
200 µg/ml
$357.00

Hello, We need to choose anti Dynactin antibody, for WB and IF in Hela cells. What is your recommendation?

Asked by: Dinaar
Thank you for your question. We offer many different Dynactin antibodies! I suggest you reach out to Technical Service, we are happy to help you with selecting which Dynactin antibody reagent would best suit your product needs.
Answered by: Tech Service
Date published: 2020-01-27

I am using Dynactin p62 (H-4): sc-55603 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from not sure-As I don't have a positive control.The sample we received was working, although there were some spotting observed (blot 1) but the purchase failed to identify a single band on insect(SF9 cell lysate expressing dynein(blot 2). The reason may be that there are no dynactin p62 in either of my protein. But the unspecific staining can not be explained.
Date published: 2019-10-28
Rated 4 out of 5 by from Good for IFThis Dynactin p62 Antibody (H-4), sc-55603, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 4 out of 5 by from Clear p62 WB bandTested endogenous p62 in HeLa cells, diluted in 1:500, got strong WB band.
Date published: 2017-06-08
Rated 5 out of 5 by from Nice p62 band had been made in our labWe had struggled for getting p62 band from other companys with upsetting results.Fortunately, Nice p62 band had been made in our lab by using this antibady(sc-55603) with 1:500 dilution in TBST.
Date published: 2016-11-02
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic stainingSuperb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-04-19
Rated 5 out of 5 by from Works very well for western blotting; testedWorks very well for western blotting; tested with RAW 264.7 whole cell lysate and murine transfected lysates. -SCBT QC
Date published: 2015-01-14
Rated 5 out of 5 by from StrongStrong, clean bands in western blot analysis of Dynactin p62 expression in H4, EOC 20, BC3H1 and Sol8 whole cell lysates. -SCBT QC
Date published: 2015-01-13
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Dynactin p62 抗体 (H-4) is rated 4.1 out of 5 by 7.
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