Date published: 2025-9-9
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DRP1 Antibody (C-5) - Western Blotting - Image 353277
DRP1 抗体 (C-5): sc-271583. K-562 (A), A549 (B), U-251-MG (C), NIH/3T3 (D) 和 C6 (E) 全细胞裂解液中 DRP1 表达的 Western 印迹分析.
DRP1 抗体 (C-5): sc-271583. K-562 (A), A549 (B), U-251-MG (C), NIH/3T3 (D) 和 C6 (E) 全细胞裂解液中 DRP1 表达的 Western 印迹分析.

DRP1 抗体 (C-5): sc-271583

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说明书
  • DRP1 抗体 C-5 是小鼠单克隆 IgG1 κ，DRP1抗体, 在149篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列560-736位于human物种的DRP1的C-terminus
  • DRP1 抗体 (C-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 all DRP1 isoforms
  • 抗DRP1抗体（C-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DRP1 (C-5): sc-271583的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是DRP1 Antibody (C-5) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与DRP1 Antibody (C-5) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

DRP抗体

DRP1抗体（C-5）是一种IgG1 κ小鼠单克隆DRP1抗体（也称为DNM1L抗体或Dymple抗体），可通过WB、IP、IF、IHC（P）和ELISA检测来自小鼠、大鼠和人的DRP1蛋白。DRP1抗体（C-5）既有非偶联抗DRP1抗体形式，也有多种偶联形式的抗DRP1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Dynamin相关蛋白1（DRP1）介导哺乳动物细胞的外线粒体膜裂变。DRP1也被称为Dynamin样蛋白1（Dlp1）、DVLP或Dymple。DRP1包含Dynamin超家族GTP酶特有的N端三分子GTP结合域。DRP1以T形二聚体的形式存在，包含头部、腿部和茎部。GTP的加入诱导头部和茎部的重新排列，产生一种力，最终导致膜收缩。DRP1在骨骼肌、心脏、肾脏和大脑中表达丰富，无处不在。在细胞中，DRP1定位于核周区域。在小鼠大脑中，DRP1在小脑中高度表达，尤其是在小脑浦肯野细胞中。在细胞凋亡过程中，DRP1从细胞质转移到线粒体，并定位到潜在的细胞器分裂位点。DRP抑制可避免细胞死亡，这表明线粒体裂变在细胞凋亡中起着关键作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DRP1 抗体 (C-5) 参考文献:

  1. 哺乳动物细胞的线粒体分裂需要Dynamin相关蛋白Drp1。  |  Smirnova, E., et al. 2001. Mol Biol Cell. 12: 2245-56. PMID: 11514614
  2. 收缩状态下动敏的三维重建。  |  Zhang, P. and Hinshaw, JE. 2001. Nat Cell Biol. 3: 922-6. PMID: 11584275
  3. 线粒体裂变介质--达纳明相关蛋白 1 在细胞凋亡中的作用  |  Frank, S., et al. 2001. Dev Cell. 1: 515-25. PMID: 11703942
  4. 一种与达能素相关的小鼠线粒体 GTPase 的初级结构及其在大脑中的分布, 亚细胞定位和对线粒体形态的影响。  |  Misaka, T., et al. 2002. J Biol Chem. 277: 15834-42. PMID: 11847212
  5. 哺乳动物细胞中线粒体形态受膜电位, DRP1依赖性分裂和FZO1依赖性融合反应的调控。  |  Ishihara, N., et al. 2003. Biochem Biophys Res Commun. 301: 891-8. PMID: 12589796
  6. 多聚酶动态蛋白样 GTPase Drp1 的 C 端 GTPase 效应域的分子内和分子间相互作用。  |  Zhu, PP., et al. 2004. J Biol Chem. 279: 35967-74. PMID: 15208300
  7. 哺乳动物线粒体裂变和融合介质 Fis1, Drp1 和 Opa1 在细胞凋亡中的作用。  |  Lee, YJ., et al. 2004. Mol Biol Cell. 15: 5001-11. PMID: 15356267
  8. Drp1/Fis1相互作用介导脓毒症心肌病的线粒体功能障碍  |  Haileselassie, B., et al. 2019. J Mol Cell Cardiol. 130: 160-169. PMID: 30981733
  9. 在卵母细胞减数分裂I期，CENP-F依赖的DRP1功能调节APC/C活性。  |  Zhou, CJ., et al. 2022. Nat Commun. 13: 7732. PMID: 36513638
  10. 通过靶向DRP1限制线粒体的可塑性，诱导代谢重编程并减少乳腺癌脑转移。  |  Parida, PK., et al. 2023. Nat Cancer. 4: 893-907. PMID: 37248394
  11. 与酵母 Vps1p 和 Dnm1p 同源的新型哺乳动物达因明相关蛋白的鉴定和亚细胞定位  |  Shin, HW., et al. 1997. J Biochem. 122: 525-30. PMID: 9348079
  12. 一种与酵母动态蛋白样 GTPase Vps1p 高度相关的新型人类蛋白质的鉴定和功能表征。  |  Imoto, M., et al. 1998. J Cell Sci. 111 (Pt 10): 1341-9. PMID: 9570752

订购信息

产品名称产品编号规格价格数量收藏夹

DRP1 抗体 (C-5)

sc-271583
200 µg/ml
$316.00

DRP1 (C-5): m-IgGκ BP-HRP 套装

sc-521987
200 µg Ab, 40 µg BP
$354.00

DRP1 (C-5): m-IgG1 BP-HRP 套装

sc-533512
200 µg Ab; 20 µg BP
$354.00

DRP1 抗体 (C-5) AC

sc-271583 AC
500 µg/ml, 25% agarose
$416.00

DRP1 抗体 (C-5) HRP

sc-271583 HRP
200 µg/ml
$316.00

DRP1 抗体 (C-5) FITC

sc-271583 FITC
200 µg/ml
$330.00

DRP1 抗体 (C-5) PE

sc-271583 PE
200 µg/ml
$343.00

DRP1 抗体 (C-5) Alexa Fluor® 488

sc-271583 AF488
200 µg/ml
$357.00

DRP1 抗体 (C-5) Alexa Fluor® 546

sc-271583 AF546
200 µg/ml
$357.00

DRP1 抗体 (C-5) Alexa Fluor® 594

sc-271583 AF594
200 µg/ml
$357.00

DRP1 抗体 (C-5) Alexa Fluor® 647

sc-271583 AF647
200 µg/ml
$357.00

DRP1 抗体 (C-5) Alexa Fluor® 680

sc-271583 AF680
200 µg/ml
$357.00

DRP1 抗体 (C-5) Alexa Fluor® 790

sc-271583 AF790
200 µg/ml
$357.00

We would like to perform immunoprecipitation，IP with this DRP1 antibody , what dilution should I use for, than you

Asked by: Muse
Thank you for your question. It would be helpful if you could email us directly at asia@scbio.cn. So we can have a more interactive discussion about this and other related issues.
Answered by: Zhanglong
Date published: 2025-04-07

I did IHC staining using these DRP1 (sc-271583), Mfn2 (sc-100560) & SOD2 (sc-137254) antibodies on mouse kidney tissues. I did not get any markers worked with your protocol (immunoperoxidase staining). 1:50 & 1:100 dilution- primary Ab 1:100- sec. Ab

Asked by: Nidhi
Thank you for contacting Santa Cruz Biotechnology. To trouble shoot please email scbt@scbt.com or call 800-457-3801 to speak with technical service.
Answered by: BlakeJ
Date published: 2024-09-20

We bought the antibody a long time ago and despite it working very well, we wanted to know what your explanation is for the double band that appears. It always happens to us using an osteosarcoma cell line. This double band also appears in your images on

Asked by: Sonia
Thank you for your question. DRP1 protein has several truncated alternate splice variants, that can be detected by the antibody, but map at a lower molecular weight than the protein's canonical form. If you have concerns about the antibody's age, please get in touch with our technical service team directly.
Answered by: Tech Support Europe
Date published: 2024-04-19

Just a quick question...the predicted band should be around 82KDa. I see a very clear band at right below 75KDa. The example by the user above was also below 75. Any help would be appreciated. Thanks

Asked by: pri1
Thank you for your question. We have not seen the band quite that low, but I believe you are seeing the right band as this is a very specific and strong antibody. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com, and we would be happy to help you figure out why your band may appear lower than expected. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible.
Answered by: Tech Service
Date published: 2017-06-16

Is it possible to use the AC conjugated DRP1 Antibody (C-5): sc-271583 AC in both Western Blot and IP?

Asked by: jenni
Thank you for your inquiry. We do not recommend the use of the AC conjugated form in Western blot. The AC conjugated form of the antibody is specifically used for IP. The unconjugated form of the antibody is the best choice when working in multiple applications.
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 4 out of 5 by from Ottimo funzionamentoAbbiamo ordinato il campione omaggio e l'abbiamo testato in western blot diluito 1:500 O.N. su lista provenienti da linee PEL. Ha dato un segnale forte e ben delineato, sicuramente si può spingere ulteriormente la diluizione.
Date published: 2024-12-20
Rated 5 out of 5 by from Excellent antibodyThe antibody is very good, giving a noticeable signal (dilution 1:1000) when using mitochondrial and post-mitochondrial fraction as sample. A small amount of sample can be used and the signal is still detectable (ranging from 15 to 25 ug of protein).
Date published: 2024-01-04
Rated 5 out of 5 by from Good for WBProvides a strong signal for WB (tested in human cell lines, 1:1000 dilution).
Date published: 2023-10-15
Rated 5 out of 5 by from DRP1 works very well in IFDRP1 works very well in IF in human tissues by histology.
Date published: 2023-05-16
Rated 4 out of 5 by from good in WBGood-- We can get the DRP1 image band from WB experiment.
Date published: 2022-10-31
Rated 4 out of 5 by from works well on my blotsI tried this antibody on my mouse and CHO cell samples. It works well on mouse samples. The band is relatively weak on CHO cells, but still seeable.(dilution 1:500)
Date published: 2022-02-13
Rated 5 out of 5 by from It worked greatIt works great without background. The dilution I used was 1:500.
Date published: 2021-10-25
Rated 4 out of 5 by from The antibody works in WBThe antobody works as expected in WB with PC12 whole cell lysates.
Date published: 2021-05-04
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DRP1 抗体 (C-5) is rated 4.5 out of 5 by 44.
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