Date published: 2025-9-7
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Dnmt3b Antibody (G-9) - Western Blotting - Image 379818
Dnmt3b 抗体 (G-9): sc-376043. A549 (A), NTERA-2 cl. D1 (B), F9 (C) 和 C6 (D) 全细胞裂解液中 Dnmt3b 表达的 Western 印迹分析.
Dnmt3b 抗体 (G-9): sc-376043. A549 (A), NTERA-2 cl. D1 (B), F9 (C) 和 C6 (D) 全细胞裂解液中 Dnmt3b 表达的 Western 印迹分析.

Dnmt3b 抗体 (G-9): sc-376043

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说明书
  • Dnmt3b 抗体 G-9 是小鼠单克隆 IgG1 κ，Dnmt3b抗体, 在35篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-230位于human物种的Dnmt3b的N-terminal附近
  • Dnmt3b 抗体 (G-9) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Dnmt3b
  • 抗Dnmt3b抗体（G-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Dnmt3b (G-9): sc-376043的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Dnmt3b Antibody (G-9) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Dnmt3b Antibody (G-9) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外Dnmt抗体，包括Dnmt1, Dnmt2, Dnmt3a, Dnmt3b和Dnmt3L

Dnmt3b抗体（G-9）是一种IgG1 κ小鼠单克隆Dnmt3b抗体（也称为DNMT3b抗体或DNA甲基转移酶3B抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的Dnmt3b蛋白。Dnmt3b抗体（G-9）既可作为非偶联抗Dnmt3b抗体形式提供，也可作为多种偶联形式的抗Dnmt3b抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。胞嘧啶5'位置的甲基化是已知的唯一自然发生的哺乳动物基因组共价修饰。DNA甲基化需要DNA 5-胞嘧啶甲基转移酶（Dnmt）蛋白的酶活性，该酶催化将甲基从S-腺苷甲硫氨酸转移到位于双核苷酸CpG基序中的胞嘧啶的5'位置，这种甲基化导致目标基因的转录抑制。Dnmt酶由独立基因编码。Dnmt1是最丰富的，它优先甲基化半甲基化DNA，并在发育过程中协调基因表达。额外的哺乳动物Dnmt蛋白包括Dnmt2和Dnmt3。Dnmt2缺乏Dnmt1的大N端调节域，在成年组织中的表达水平低得多，并且可能参与新整合的逆转录病毒DNA的甲基化。Dnmt3a和Dnmt3b由两个不同的基因编码，但两者都在胚胎干细胞中大量表达，它们也在DNA上甲基化CpG基序。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Dnmt3b 抗体 (G-9) 参考文献:

  1. DNA 甲基转移酶在早期发育过程中被积极地保留在细胞质中。  |  Cardoso, MC. and Leonhardt, H. 1999. J Cell Biol. 147: 25-32. PMID: 10508852
  2. 小鼠从头甲基转移酶 Dnmt3a 和 Dnmt3b 的体内活性。  |  Hsieh, CL. 1999. Mol Cell Biol. 19: 8211-8. PMID: 10567546
  3. DNA 甲基转移酶 Dnmt1 与组蛋白去乙酰化酶的活性有关。  |  Fuks, F., et al. 2000. Nat Genet. 24: 88-91. PMID: 10615135
  4. 人类 DNA 甲基转移酶（dnmt1）基因的转录调控。  |  Bigey, P., et al. 2000. Gene. 242: 407-18. PMID: 10721735
  5. 在转基因D4Z4小鼠中，Dnmt3b以组织依赖性的方式调节DUX4的表达。  |  Bouwman, LF., et al. 2020. Skelet Muscle. 10: 27. PMID: 33004076
  6. DNMT3B沉默通过表观遗传学促进膀胱癌中的miR-34a，抑制迁移和侵袭。  |  Xu, K., et al. 2020. Aging (Albany NY). 12: 23668-23683. PMID: 33221743
  7. DNMT3B通过microRNA-152-3p/NCAM1通路调节A549细胞的增殖。  |  Yang, B., et al. 2022. Oncol Lett. 23: 11. PMID: 34820010
  8. 棕色脂肪 Dnmt3b 缺失可改善雌性小鼠的肥胖症。  |  Li, F., et al. 2021. Life (Basel). 11: PMID: 34947856
  9. PTBP1通过与RALY相互作用来调节DNMT3B的替代性剪接，从而增强前列腺癌的放射抗性。  |  He, H., et al. 2024. Adv Sci (Weinh). 11: e2405997. PMID: 39287090
  10. 小鼠细胞和组织中的 DNA（胞嘧啶-5）-甲基转移酶。使用基于机制的探针进行研究。  |  Yoder, JA., et al. 1997. J Mol Biol. 270: 385-95. PMID: 9237905
  11. 胚胎干细胞中病毒 DNA 的从头甲基化和维持甲基化不需要 Dnmt2。  |  Okano, M., et al. 1998. Nucleic Acids Res. 26: 2536-40. PMID: 9592134
  12. 胞嘧啶甲基化与哺乳动物的发育  |  Walsh, CP. and Bestor, TH. 1999. Genes Dev. 13: 26-34. PMID: 9887097

订购信息

产品名称产品编号规格价格数量收藏夹

Dnmt3b 抗体 (G-9)

sc-376043
200 µg/ml
$316.00

Dnmt3b (G-9): m-IgG Fc BP-HRP 套装

sc-529809
200 µg Ab; 10 µg BP
$354.00

Dnmt3b (G-9): m-IgGκ BP-HRP 套装

sc-522829
200 µg Ab, 40 µg BP
$354.00

Dnmt3b (G-9): m-IgG1 BP-HRP 套装

sc-543700
200 µg Ab; 20 µg BP
$354.00

Dnmt3b 抗体 (G-9) AC

sc-376043 AC
500 µg/ml, 25% agarose
$416.00

Dnmt3b 抗体 (G-9) HRP

sc-376043 HRP
200 µg/ml
$316.00

Dnmt3b 抗体 (G-9) FITC

sc-376043 FITC
200 µg/ml
$330.00

Dnmt3b 抗体 (G-9) PE

sc-376043 PE
200 µg/ml
$343.00

Dnmt3b 抗体 (G-9) Alexa Fluor® 488

sc-376043 AF488
200 µg/ml
$357.00

Dnmt3b 抗体 (G-9) Alexa Fluor® 546

sc-376043 AF546
200 µg/ml
$357.00

Dnmt3b 抗体 (G-9) Alexa Fluor® 594

sc-376043 AF594
200 µg/ml
$357.00

Dnmt3b 抗体 (G-9) Alexa Fluor® 647

sc-376043 AF647
200 µg/ml
$357.00

Dnmt3b 抗体 (G-9) Alexa Fluor® 680

sc-376043 AF680
200 µg/ml
$357.00

Dnmt3b 抗体 (G-9) Alexa Fluor® 790

sc-376043 AF790
200 µg/ml
$357.00

Dear Sir/Madam, I ordered Dnmt3b Antibody (G-9) sc-376043 (Lot # F0920) for western hybridisation in mouse ESC. I could not see any band, no matter 1:200 or 1:1000 dilution. Could you please replace or send me vials of sc-81252 and sc-393845 free sample?

Asked by: Li Ding
Thank you for your question. Please contact technical service directly for troubleshooting advice.
Answered by: Tech Support Europe
Date published: 2021-03-05

Can Dnmt3b (G-9): sc-376043 be frozen?

Asked by: jenniferc
No. The antibody solution contains gelatin. When the gelatin is frozen the antibody precipitates out of solution. However, if an antibody’s datasheet says that the antibody should be stored frozen, then the antibody can be frozen, as these antibodies do not contain gelatin.
Answered by: Technical Support 7
Date published: 2017-01-24
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Rated 2 out of 5 by from Not clear bandI did not see a clear band by western blot (1:1000 dilution) in glioblastoma cell lines
Date published: 2022-02-16
Rated 1 out of 5 by from non funzionanon si vede nessuna banda, pur provando diluizioni diverse e tipi diversi di blocking
Date published: 2019-05-15
Rated 3 out of 5 by from One sharp band but went bad after 1 yearIt showed one sharp band in the beginning. However, I had to use fresh antibody every time at 1:500 dilution. More importantly, it does not work anymore after 1 year
Date published: 2019-05-07
Rated 1 out of 5 by from HRP version of this antibody doesn't workI previously used the original G-9 version of this antibody and we recently purchased the version bound to HRP to have faster results. To our surprise, the signal is really bad and can be barely seen in our membranes, even with long exposure times. I tried contacting the company via e-mail about the issue to have a replacement without success.
Date published: 2018-02-08
Rated 4 out of 5 by from Ottimo funzionamentoBuona specificità e sensibilità dell'Ab alle condizioni suggerite nel datasheet
Date published: 2017-11-20
Rated 4 out of 5 by from not very goodI don't get good band, maybe I used cell line. I can get good result.
Date published: 2017-08-19
Rated 5 out of 5 by from very nice bandThe antibody has been tried in western blot on rat tissue samples. Worked well at a 1/1000 dilution.
Date published: 2017-07-07
Rated 4 out of 5 by from Good antibody for Western blotWorks! I tested and validated this antibody using a knockout cell line (see image). Best used at 1:500 in 3% BSA. Exposure 10-30 times longer than tubulin (for your reference).
Date published: 2017-06-23
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Dnmt3b 抗体 (G-9) is rated 3.7 out of 5 by 12.
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