Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DNCLI1 CRISPR/Cas9 KO Plasmid (h): sc-412128
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DNCLI1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the DNCLI1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DNCLI1 Antibody (H-7): sc-514141
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DNCLI1 CRISPR/Cas9 KO Plasmid (h) | sc-412128 | 20 µg | $397.00 |
Overview
DYNC1LI1 encodes dynein cytoplasmic 1 light intermediate chain 1 (DNCLI1), a core subunit of the cytoplasmic dynein-1 motor complex that drives minus-end–directed transport along microtubules. DNCLI1 contributes to cargo binding and motor regulation, supporting processes such as vesicle and organelle trafficking, endosome–lysosome positioning, Golgi organization, and retrograde axonal transport. Through these roles, DYNC1LI1 influences cell polarity, mitotic spindle dynamics, and neuronal homeostasis, linking dynein-dependent transport defects to neurodevelopmental and neurodegenerative disease mechanisms. Altered dynein complex function is also relevant to pathways governing stress responses and intracellular signaling that depend on accurate cargo localization.
DNCLI1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DYNC1LI1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DYNC1LI1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DYNC1LI1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish DNCLI1 protein expression.
This CRISPR knockout system enables efficient generation of DYNC1LI1-deficient cell models for investigation of DNCLI1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DYNC1LI1 exon(s) critical for DNCLI1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DYNC1LI1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by DNCLI1 CRISPR/Cas9 KO Plasmid (h) and DNCLI1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DYNC1LI1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by DNCLI1 HDR Plasmid (h) and DNCLI1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DYNC1LI1 homology arms to support homology-directed repair at defined DYNC1LI1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.