Date published: 2025-9-8
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DNase I Antibody (B-4) - Western Blotting - Image 393071
DNase I 抗体 (B-4) HRP: sc-376207 HRP. F9 (A), NIH/3T3 (B), C6 (C) 和 SH-SY5Y (D) 全细胞裂解液中 DNase I 表达的直接 Western 印迹分析.
DNase I 抗体 (B-4) HRP: sc-376207 HRP. F9 (A), NIH/3T3 (B), C6 (C) 和 SH-SY5Y (D) 全细胞裂解液中 DNase I 表达的直接 Western 印迹分析.

DNase I 抗体 (B-4): sc-376207

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说明书
  • DNase I 抗体 B-4 是小鼠单克隆 IgG1 κ，DNase I抗体, 在3篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的DNase I的内部区域内的氨基酸73-91之间
  • DNase I 抗体 (B-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 DNase I
  • 抗DNase I抗体（B-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DNase I (B-4): sc-376207的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 DNase I 抗体 (B-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与DNase I 抗体 (B-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 DNases 抗体 包括 DNASE1L1

DNase I 抗体（B-4）是一种 IgG1 κ 鼠单克隆 DNase I 抗体（也称为 DNase I 抗体），可通过 WB、IP、IF、IHC(P) 和 ELISA 方法检测小鼠、大鼠和人类来源的 DNase I 蛋白质。DNase I 抗体（B-4）既可以是非偶联的抗 DNase I 抗体形式，也可以是多种偶联形式的抗 DNase I 抗体，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。核酸内切酶激活后的核小体间 DNA 片段化是细胞凋亡的共同终点。DNase I 是一种在哺乳动物组织中普遍表达的 Ca2+/Mg2+ 依赖性核酸内切酶，已被证实可介导人细胞凋亡过程中的核小体间 DNA 降解。DNase I 具有高度多态性，目前已知至少有六种 DNase I 等位基因。DNase II 是一种普遍表达的酸性脱氧核糖核酸酶，在 caspase 激活后发挥作用，也可能在细胞凋亡过程中诱导 DNA 消化。DNase I 将 DNA 裂解为 5'-磷酸二核苷酸和 5'-磷酸寡核苷酸终产物，而 DNase II 则将 DNA 裂解为 3'-磷酸单核苷酸和 3'-磷酸寡核苷酸终产物。DNase II 切割 DNA 的机制与 DNase I 相似，产生的是切口而非双链切割。DNase II 通常存在于上皮细胞的细胞质中，但在晶状体纤维的细胞核中似乎更为集中。相比之下，DNase I 总是集中在上皮和纤维细胞的细胞核中。编码 DNase II 的基因定位于人类 19 号染色体上。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DNase I 抗体 (B-4) 参考文献:

  1. 人类脱氧核糖核酸酶 I 多态性的一个新等位基因 DNASE1*6 编码 Arg 到 Cys 的置换，导致其不稳定。  |  Yasuda, T., et al. 1999. Biochem Biophys Res Commun. 260: 280-3. PMID: 10381379
  2. 人类 DNase I 家族内切酶的特征和凋亡过程中 DNase gamma 的激活。  |  Shiokawa, D. and Tanuma, S. 2001. Biochemistry. 40: 143-52. PMID: 11141064
  3. DNase I 在药物诱导凋亡的人类细胞中介导核糖体 DNA 降解。  |  Oliveri, M., et al. 2001. Eur J Immunol. 31: 743-51. PMID: 11241278
  4. 糖尿病患者的 DNase I 活性增加可能与胰腺损伤有关。  |  Zhu, B., et al. 2014. Mol Cell Biochem. 393: 23-32. PMID: 24676545
  5. 利用基因表达对 DNase I 高敏感性进行全基因组预测。  |  Zhou, W., et al. 2017. Nat Commun. 8: 1038. PMID: 29051481
  6. 抗坏血酸作为 DNase I 抑制剂预防男性不育。  |  Ilić, BS., et al. 2018. Biochem Biophys Res Commun. 498: 1073-1077. PMID: 29551685
  7. 一些 4-噻唑烷酮衍生物的合成和 DNase I 抑制特性。  |  Kolarević, A., et al. 2019. J Cell Biochem. 120: 264-274. PMID: 30171712
  8. 生物启发的 DNase-I 涂层类黑色素纳米球用于调节感染相关的 NETosis 失调。  |  Park, HH., et al. 2021. Adv Sci (Weinh). 8: e2103748. PMID: 34623037
  9. DNase II 参与了晶状体细胞分化过程中的核变性。  |  Torriglia, A., et al. 1995. J Biol Chem. 270: 28579-85. PMID: 7499373
  10. 编码人类脱氧核糖核酸酶 II 的 cDNA 的分子克隆。  |  Yasuda, T., et al. 1998. J Biol Chem. 273: 2610-6. PMID: 9446563
  11. 人类和鼠类 DNase II 的分子克隆和特征描述。  |  Baker, KP., et al. 1998. Gene. 215: 281-9. PMID: 9714827
  12. 人脱氧核糖核酸酶 II 的克隆和表达。在细胞凋亡中可能发挥的作用  |  Krieser, RJ. and Eastman, A. 1998. J Biol Chem. 273: 30909-14. PMID: 9812984

订购信息

产品名称产品编号规格价格数量收藏夹

DNase I 抗体 (B-4)

sc-376207
200 µg/ml
$316.00

DNase I (B-4): m-IgG Fc BP-HRP 套装

sc-529837
200 µg Ab; 10 µg BP
$354.00

DNase I (B-4): m-IgGκ BP-HRP 套装

sc-522875
200 µg Ab, 40 µg BP
$354.00

DNase I (B-4): m-IgG1 BP-HRP 套装

sc-543716
200 µg Ab; 20 µg BP
$354.00

DNase I 抗体 (B-4) AC

sc-376207 AC
500 µg/ml, 25% agarose
$416.00

DNase I 抗体 (B-4) HRP

sc-376207 HRP
200 µg/ml
$316.00

DNase I 抗体 (B-4) FITC

sc-376207 FITC
200 µg/ml
$330.00

DNase I 抗体 (B-4) PE

sc-376207 PE
200 µg/ml
$343.00

DNase I 抗体 (B-4) Alexa Fluor® 488

sc-376207 AF488
200 µg/ml
$357.00

DNase I 抗体 (B-4) Alexa Fluor® 546

sc-376207 AF546
200 µg/ml
$357.00

DNase I 抗体 (B-4) Alexa Fluor® 594

sc-376207 AF594
200 µg/ml
$357.00

DNase I 抗体 (B-4) Alexa Fluor® 647

sc-376207 AF647
200 µg/ml
$357.00

DNase I 抗体 (B-4) Alexa Fluor® 680

sc-376207 AF680
200 µg/ml
$357.00

DNase I 抗体 (B-4) Alexa Fluor® 790

sc-376207 AF790
200 µg/ml
$357.00

DNase I (B-4) 中和勝肽

sc-376207 P
100 µg/0.5 ml
$68.00

Is biotin-conjugated DNase I Antibody (B-4): sc-376207 available? If not available, can you make it available? Thanks

Asked by: Atsuoo
Thank you for your question. Please contact our Technical Service Department for assistance.
Answered by: Technical Service 4
Date published: 2020-12-18

Does this antibody require antigen retrieval prior to using on formalin fixed paraffin embedded tissue?

Asked by: kimos
Thank you for your question. Yes, for this antibody, DNase I Antibody (B-4): sc-376207, we recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found on our website using the link below. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-14

What application is the blocking peptide sc-376207 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Antiboy does not workI testet the Dnase1 B-4 antibody in kidney lysates of Dnase1 WT and KO mice. I faced multiple unspecific binding in WT and KO lysates and neither of the bands was on the correct size.
Date published: 2017-04-20
Rated 5 out of 5 by from Nice antibodyNice and clear bands with DNase I Antibody (B-4). Also, specific staining observed by IHCP and IF
Date published: 2017-01-25
Rated 5 out of 5 by from Nice cytoplasmic staining of the DNase lNice cytoplasmic staining of the DNase l protein by immunofluorescence of methanol fixed HeLa cells. -SCBT QC
Date published: 2015-07-07
Rated 3 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-05-09
Rated 5 out of 5 by from Produced a clear band with MCF7 and HeLaProduced a clear band with MCF7 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-04-19
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmicProduced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human small intestine tissue. -SCBT QC
Date published: 2015-01-12
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DNase I 抗体 (B-4) is rated 4.0 out of 5 by 6.
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