Dkk-1抗体 (B-7) 推荐用于 mouse, rat 和 human 来源的Dkk-1 WB, IP, IF, IHC(P) 和 ELISA检测

Dkk-1 抗体 (B-7): sc-374574

Dkk-1抗体 (B-7) is rated 4.4 out of 5 by 11.
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说明书

    • 抗-Dkk-1 抗体 B-7 是小鼠单克隆 IgG1 κ,Dkk-1抗体, 在19篇文献中引用,规格为200 µg/ml
    • 免疫human物种的Dkk-1的氨基酸1-120
    • 抗-Dkk-1 抗体 (B-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Dkk-1
    • 抗-Dkk-1 抗体 (B-7) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Dkk-1 (B-7): sc-374574免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP)m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) 是 Dkk-1 Antibody (B-7) for WB and IHC(P) 首选应用二抗. 这些试剂现在以套形式提供 Dkk-1 Antibody (B-7) (见下方订购信息).

Can this antibody be used for FACS sorting?.

Asked by: ElenaR
Thank you for your question. We have not yet tested this mouse monoclonal, Dkk-1 Antibody (B-7): sc-374574, for FACS or FCM so this application is not recommended at this time. We do offer this antibody directly conjugated to phycoerythrin (sc-374574 PE), fluorescein (sc-374574 FITC), Alexa Fluor® 488 (sc-374574 AF488) or Alexa Fluor® 647 (sc-374574 AF647), 200 µg/ml. If you purchase one of these direct conjugates we will warranty the antibody for FCM.
Answered by: Technical Support
Date published: 2017-03-03

What is the total volume provided of Dkk-1 (B-7):sc-374574 monoclonal antibody?

Asked by: jenni
Thank you for your question. This antibody is provided as 200 µg in 1 ml total volume of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 4
Date published: 2017-01-13

Can Dkk-1 (B-7): sc-374574 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Dkk-1 (B-7): sc-374574 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 4
Date published: 2017-01-13

Buenas tardes, necesitamos determinar DKK1 mediante un inmunoensayo sobre un electrodo (inmunosensor electroquímico), para lo cual necesitaríamos un anticuerpo de captura, la interleucina DKK1 y un anticuerpo marcado, por ejemplo con Biotina o HRP.

Asked by: Anonymous
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Rated 5 out of 5 by from Worked great Worked great for immunofluorescence on paraffin-embedded murine samples.
Date published: 2020-10-06
Rated 4 out of 5 by from Good for WB In MCF7 cells, a spcific band was found in WB, diluted at 1:1000.
Date published: 2018-04-12
Rated 5 out of 5 by from Great features! I applied for a free sample two weeks ago,then western blot detection dkk-1 of mouse. dilution:1:100 The experimental results are good!
Date published: 2017-09-18
Rated 4 out of 5 by from 需要进一步验证 两个月前申请试用装,在皮肤中进行免疫荧光实验检测,做出了疑似阳性结果,还没有重复,希望能对大家有所帮助。
Date published: 2017-08-24
Rated 5 out of 5 by from Western Blot This antibody is very clean in Western Blot with almost no additional bands, and those present are very faint.
Date published: 2017-04-15
Rated 5 out of 5 by from Outstanding product Very strong antibody for both western blot and IHC applications !!
Date published: 2017-01-20
Rated 5 out of 5 by from Good for IHC This Dkk-1 antibody shows good results for IHC staining
Date published: 2017-01-12
Rated 4 out of 5 by from good antibody Clear bands were obtained of porcine cells protein extraction western blotting.
Date published: 2016-11-22
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