Date published: 2025-9-8
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Dicer Antibody (F-10) - Western Blotting - Image 70464
Dicer 抗体 (F-10): sc-136979. AMJ2-C11 (A), NIH/3T3 (B), RPE-J (C) 和 KNRK (D) 全细胞裂解液及大鼠脑组织提取物 (E) 中 Dicer 表达的 Western 印迹分析.
Dicer 抗体 (F-10): sc-136979. AMJ2-C11 (A), NIH/3T3 (B), RPE-J (C) 和 KNRK (D) 全细胞裂解液及大鼠脑组织提取物 (E) 中 Dicer 表达的 Western 印迹分析.

Dicer 抗体 (F-10): sc-136979

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说明书
  • Dicer 抗体 F-10 是小鼠单克隆 IgG2a κ，Dicer抗体, 在16篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1701-1912，在Dicer的human起源的C-terminus处进行映射
  • Dicer 抗体 (F-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Dicer
  • 抗Dicer抗体（F-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Dicer (F-10): sc-136979的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 Dicer 抗体 (F-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Dicer 抗体 (F-10) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Dicer 抗体包括 Dicer-1

Dicer抗体（F-10）是一种IgG2a κ小鼠单克隆Dicer抗体（也称为DICER1抗体或dicer 1核糖核酸酶III抗体），可通过WB、IP、IF、IHC(P)和ELISA检测到小鼠、大鼠和人类来源的Dicer蛋白。Dicer抗体（F-10）既可用作非偶联形式的抗Dicer抗体，也可用作多种偶联形式的抗Dicer抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。哺乳动物的Dicer是一种与III型RNase相关的蛋白质，在酵母、果蝇和拟南芥中具有同源物。Dicer在其氨基末端含有一个RNA解旋酶基序，包括一个DEXH盒，在其羧基末端含有一个RNase基序。编码人类Dicer的基因位于14q31染色体上。Dicer在脑、心、肝、肺、胰、肾和胎盘中表达，并在RNA干扰途径中发挥作用。Dicer将大约70bp的短发夹RNA前体切割成21-23bp的dsRNAs，这些dsRNAs选择性地靶向同源RNA的破坏。Dicer定位于哺乳动物细胞的细胞质中。具体来说，它与内质网中的钙网蛋白共定位。尽管Dicer对RNA的切割不依赖于ATP，但这种酶快速周转所需的产物释放可能归因于ATP。免疫沉淀研究表明，Dicer与eIF2C翻译起始因子的PIWI结构域形成复合物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Dicer 抗体 (F-10) 参考文献:

  1. 新型人类基因（HERNA）的分子克隆和特征描述，该基因编码一种假定的 RNA 螺旋酶。  |  Matsuda, S., et al. 2000. Biochim Biophys Acta. 1490: 163-9. PMID: 10786632
  2. 长双链 RNA 对小鼠胚胎畸胎癌细胞系基因表达的特异性干扰。  |  Billy, E., et al. 2001. Proc Natl Acad Sci U S A. 98: 14428-33. PMID: 11724966
  3. 短发夹核糖核酸（shRNA）在哺乳动物细胞中诱导序列特异性沉默。  |  Paddison, PJ., et al. 2002. Genes Dev. 16: 948-58. PMID: 11959843
  4. 重组人 Dicer 的核糖核酸酶活性和 RNA 结合。  |  Provost, P., et al. 2002. EMBO J. 21: 5864-74. PMID: 12411504
  5. 人类 Dicer 在不需要 ATP 的情况下优先在 dsRNA 的末端进行切割。  |  Zhang, H., et al. 2002. EMBO J. 21: 5875-85. PMID: 12411505
  6. 从结构和功能的角度看"Dicer生物学"。  |  Paturi, S. and Deshmukh, MV. 2021. Front Mol Biosci. 8: 643657. PMID: 34026825
  7. DICER产生小RNA的序列决定因素。  |  Lee, YY., et al. 2023. Nature. 615: 323-330. PMID: 36813957
  8. Dicer的结构和功能：保守和进化的特征。  |  Zapletal, D., et al. 2023. EMBO Rep. 24: e57215. PMID: 37310138
  9. DICER核糖核酸酶可消除有害的R环。  |  Camino, LP., et al. 2023. Mol Cell. 83: 3707-3719.e5. PMID: 37827159
  10. DICER的pre-miRNA切割分析。  |  Le, CT., et al. 2023. Methods Enzymol. 692: 231-247. PMID: 37925182
  11. 针对 RNA 病毒的抗病毒防御，Argonaute 独立，Dicer 依赖。  |  Sato, Y., et al. 2024. Proc Natl Acad Sci U S A. 121: e2322765121. PMID: 38865263
  12. 哺乳动物细胞中短干扰 RNA 介导的基因沉默需要 Dicer 和 eIF2C 翻译引发剂。  |  Doi, N., et al. 2003. Curr Biol. 13: 41-6. PMID: 12526743

订购信息

产品名称产品编号规格价格数量收藏夹

Dicer 抗体 (F-10)

sc-136979
200 µg/ml
$316.00

Dicer (F-10): m-IgG Fc BP-HRP 套装

sc-528832
200 µg Ab; 10 µg BP
$354.00

Dicer (F-10): m-IgGκ BP-HRP 套装

sc-521380
200 µg Ab, 40 µg BP
$354.00

Dicer 抗体 (F-10) AC

sc-136979 AC
500 µg/ml, 25% agarose
$416.00

Dicer 抗体 (F-10) HRP

sc-136979 HRP
200 µg/ml
$316.00

Dicer 抗体 (F-10) FITC

sc-136979 FITC
200 µg/ml
$330.00

Dicer 抗体 (F-10) PE

sc-136979 PE
200 µg/ml
$343.00

Dicer 抗体 (F-10) Alexa Fluor® 488

sc-136979 AF488
200 µg/ml
$357.00

Dicer 抗体 (F-10) Alexa Fluor® 546

sc-136979 AF546
200 µg/ml
$357.00

Dicer 抗体 (F-10) Alexa Fluor® 594

sc-136979 AF594
200 µg/ml
$357.00

Dicer 抗体 (F-10) Alexa Fluor® 647

sc-136979 AF647
200 µg/ml
$357.00

Dicer 抗体 (F-10) Alexa Fluor® 680

sc-136979 AF680
200 µg/ml
$357.00

Dicer 抗体 (F-10) Alexa Fluor® 790

sc-136979 AF790
200 µg/ml
$357.00

Dicer Antibody (F-10): sc-136979 can i use this one as anti dicer antibody for Coimmuno precipitation application

Asked by: vijaya
Thank you for contacting Santa Cruz Biotechnology . sc-136979 Dicer (F-10) is recommended for WB, IP, PAR, ELISA.
Answered by: BlakeJ
Date published: 2025-02-21

Hi! Does this antibody work in immunofluorescence on frozen sections ? Thank you

Asked by: Alii
Thank you for your question. The antibody sc-136979 is recommended for detection of Dicer of mouse, rat and human origin by Western Blot, IP, Immunocytochemistry on methanol-fixed cells, immunohistochemistry on acetone-fixed frozen tissues sections, immunohistochemistry on paraffin embedded tissues fixed with formalin and ELISA. If you require more information, please contact our Technical Service
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

I am trying to show Dicer1 expression in mouse liver lysates by WB or IHC. Does sc-136979 work for these purposes?

Asked by: RamC
Thank you very much for the question. Dicer Antibody (F-10): sc-136979 is recommended for detection of Dicer of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. We have tested and we show a variety of Sample materials from different sources in the product literature. Yes, this product is warranted for the detection of Dicer in mouse liver lysates by WB and IHC according to our recommended protocols. https://www.scbt.com/scbt/resources/protocols
Answered by: Technical Support Europe
Date published: 2018-01-31

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: TinTin
Thanks for your question. In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody, Dicer (F-10), is currently available conjugated to agarose (sc-136979 AC), HRP (sc-136979 HRP), phycoerythrin (sc-136979 PE), fluorescein (sc-136979 FITC), Alexa Fluor® 488 (sc-136979 AF488) and Alexa Fluor® 647 (sc-136979 AF647).
Answered by: Technical Support 3
Date published: 2017-01-12

I am going to use this antibody for my WB, but I need a new secondary. What do you recommend?

Asked by: TinTin
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-12
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Rated 4 out of 5 by from Overall goodI used a 1:500 dilution. The bands were accurate although sometimes not very clear. Overall a good and accurate product.
Date published: 2024-03-04
Rated 2 out of 5 by from richiede elevata esposizionenecessita elevata esposizione ma si vede. NB: elevato MW, valutare anche modifiche nella scelta del gel e/o del blotting
Date published: 2019-05-15
Rated 5 out of 5 by from Good antibody for IHC!I applyed for the sample of this antibody for free. I performed immunohistochemistry of FFPE (formalin fixed and paraffin embedded) with this antibody (diluted 1:50) to detect the expression of dicer in human breast cancer tissues.
Date published: 2018-04-17
Rated 3 out of 5 by from 申请DICER试用装后评论我几个月前，申请了DICER小样试用，但是并没有跑出来，贵公司服务特别周到，给了很多建议，但是最终我还是没有曝出来，也有可能是我的技术操作问题。
Date published: 2017-10-02
Rated 1 out of 5 by from IHC Assay Review for Dicer mABApplication: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: No signal detected.
Date published: 2017-08-31
Rated 5 out of 5 by from Works okUsed in mouse liver tissue . Works ok with dilution 1:400,Nice band obtained.Please see image above.
Date published: 2017-08-19
Rated 5 out of 5 by from very nice bandantibody tried in western blot, on rat tissue samples. It worked very well.
Date published: 2017-07-07
Rated 5 out of 5 by from Produced publishable WB data in human and ratThis Dicer monoclonal was featured in several publications in the primary literature for the use of WB in rat brain mitochondria, rat cortical neurons and human samples, such as MCF-7 cells
Date published: 2017-01-30
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Dicer 抗体 (F-10) is rated 4.1 out of 5 by 13.
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