Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Daxx Double Nickase Plasmid (h): sc-400686-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Daxx Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Daxx Double Nickase Plasmid (h) and Daxx Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DAXX. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Daxx Antibody (H-7): sc-8043
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Daxx Double Nickase Plasmid (h) | sc-400686-NIC | 20 µg | $410.00 | |||
Daxx Double Nickase Plasmid (h2) | sc-400686-NIC-2 | 20 µg | $410.00 |
DAXX encodes Daxx, a multifunctional nuclear protein that acts as a transcriptional coregulator and chromatin-associated factor, best known for partnering with ATRX to deposit histone variant H3.3 at repetitive heterochromatin and telomeric regions. Through interactions with PML nuclear bodies and diverse transcription factors, Daxx influences chromatin organization, DNA damage responses, and stress-induced signaling pathways. Daxx has also been linked to apoptosis regulation and modulation of innate immune and interferon-related transcriptional programs. Dysregulated DAXX function or expression has been associated with altered epigenetic states and genomic instability observed in multiple disease contexts, including cancer and neurodevelopmental phenotypes.
Daxx Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DAXX locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DAXX. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DAXX function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DAXX-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.