Date published: 2025-9-6

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Daxx 抗体 (H-7): sc-8043

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说明书
  • Daxx 抗体 H-7 是小鼠单克隆 IgG2a κ,Daxx抗体, 在26篇文献中引用,规格为200 µg/ml
  • 免疫mouse来源Daxx位于C-terminus 的627-739氨基酸
  • Daxx 抗体 (H-7) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Daxx
  • 抗Daxx抗体(H-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Daxx (H-7): sc-8043免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是Daxx Antibody (H-7) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Daxx Antibody (H-7) 打包提供(请参阅下面的订购信息)。

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関連項目

Daxx抗体(H-7)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹、免疫沉淀、免疫荧光和酶联免疫吸附试验检测小鼠、大鼠和人类来源的Daxx蛋白。Daxx (H-7) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合型。Daxx 在细胞凋亡调节中起着至关重要的作用,尤其是在 FAS 介导的凋亡途径中。当FAS受体被其配体激活时,激活会引发一系列事件,导致细胞程序性死亡,这对于维持免疫系统平衡和组织稳态至关重要。Daxx是这一过程中的关键角色,因为它与FAS受体的死亡域相互作用,促进Jun N-末端激酶(JNK)的激活,并促进细胞凋亡。值得注意的是,虽然Daxx不具有死亡域,但Daxx的过度表达可以通过激活JNK途径来增强FAS介导的细胞凋亡。该途径与FAS-FADD-FLICE途径同时存在,但明显不同,后者是细胞凋亡的另一个关键途径。Daxx途径对凋亡抑制蛋白Bcl-2敏感,突出了细胞死亡中独特的调节机制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Daxx 抗体 (H-7) 参考文献:

  1. Daxx 可抑制 NF-kappaB 乙酰化及其转录活性。  |  Park, J., et al. 2007. J Mol Biol. 368: 388-97. PMID: 17362989
  2. 丙磺舒的抗凋亡作用与下调 THP-1 巨噬细胞中 Daxx 的表达有关。  |  Su, B., et al. 2007. Cardiovasc Drugs Ther. 21: 37-45. PMID: 17370120
  3. DAXX-ATRX 对 p53 染色质结合和 DNA 损伤反应的调控。  |  Gulve, N., et al. 2022. Nat Commun. 13: 5033. PMID: 36028493
  4. DAXX为组蛋白伴侣网络增加了从头H3.3K9me3沉积途径。  |  Carraro, M., et al. 2023. Mol Cell. 83: 1075-1092.e9. PMID: 36868228
  5. DAXX通过防止R环诱导的DNA双链断裂的积累,在ATRX之外促进中心粒稳定性。  |  Pinto, LM., et al. 2024. Nucleic Acids Res. 52: 1136-1155. PMID: 38038252
  6. FADD是一种新型含死亡结构域的蛋白质,它与Fas的死亡结构域相互作用,启动细胞凋亡。  |  Chinnaiyan, AM., et al. 1995. Cell. 81: 505-12. PMID: 7538907
  7. TRADD-TRAF2 和 TRADD-FADD 相互作用定义了两种不同的 TNF 受体 1 信号转导途径。  |  Hsu, H., et al. 1996. Cell. 84: 299-308. PMID: 8565075
  8. 杀人许可证  |  Fraser, A. and Evan, G. 1996. Cell. 85: 781-4. PMID: 8681372
  9. 新型与 MORT1/FADD 相互作用的蛋白酶 MACH 参与 Fas/APO-1 和 TNF 受体诱导的细胞死亡。  |  Boldin, MP., et al. 1996. Cell. 85: 803-15. PMID: 8681376
  10. 死亡因子导致的细胞凋亡  |  Nagata, S. 1997. Cell. 88: 355-65. PMID: 9039262
  11. 丝裂原活化蛋白激酶介导的 Fas 细胞凋亡信号通路。  |  Goillot, E., et al. 1997. Proc Natl Acad Sci U S A. 94: 3302-7. PMID: 9096388
  12. Daxx,一种新型 Fas 结合蛋白,可激活 JNK 和细胞凋亡。  |  Yang, X., et al. 1997. Cell. 89: 1067-76. PMID: 9215629

订购信息

产品名称产品编号规格价格数量收藏夹

Daxx 抗体 (H-7)

sc-8043
200 µg/ml
$316.00

Daxx (H-7): m-IgG Fc BP-HRP 套装

sc-528211
200 µg Ab; 10 µg BP
$354.00

Daxx (H-7): m-IgGκ BP-HRP 套装

sc-520534
200 µg Ab, 40 µg BP
$354.00

Daxx (H-7): m-IgG2a BP-HRP 套装

sc-547048
200 µg Ab; 10 µg BP
$354.00

Daxx 抗体 (H-7) AC

sc-8043 AC
500 µg/ml, 25% agarose
$416.00

Daxx 抗体 (H-7) HRP

sc-8043 HRP
200 µg/ml
$316.00

Daxx 抗体 (H-7) FITC

sc-8043 FITC
200 µg/ml
$330.00

Daxx 抗体 (H-7) PE

sc-8043 PE
200 µg/ml
$343.00

Daxx 抗体 (H-7) Alexa Fluor® 488

sc-8043 AF488
200 µg/ml
$357.00

Daxx 抗体 (H-7) Alexa Fluor® 546

sc-8043 AF546
200 µg/ml
$357.00

Daxx 抗体 (H-7) Alexa Fluor® 594

sc-8043 AF594
200 µg/ml
$357.00

Daxx 抗体 (H-7) Alexa Fluor® 647

sc-8043 AF647
200 µg/ml
$357.00

Daxx 抗体 (H-7) Alexa Fluor® 680

sc-8043 AF680
200 µg/ml
$357.00

Daxx 抗体 (H-7) Alexa Fluor® 790

sc-8043 AF790
200 µg/ml
$357.00

What is the recommended starting dilution for Western blot for Daxx (H-7): sc-8043?

Asked by: jenni
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-8043.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 14
Date published: 2025-03-24
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Rated 4 out of 5 by from Anti-Daxx AntibodyWorks well for detecting purified DAXX, the band is around 110~120KD.
Date published: 2022-01-06
Rated 5 out of 5 by from It worked well for IF in mouse ES cellsIt worked well for conventional IF with low background in mouse ES cells.
Date published: 2020-09-29
Rated 4 out of 5 by from Work for mouse breast cancer cell linesWork for IF and WB in mouse. The signal is clear but relatively weak.
Date published: 2018-02-20
Rated 1 out of 5 by from Daxx ab for wbI got free sample from this ab and tested with 5 different mouse cell lines and unfortunately it did not work for me.
Date published: 2017-12-07
Rated 3 out of 5 by from DaxxSignal was good, detected molecular weight less than 130 KDA in Esophageal cancer cell
Date published: 2017-11-13
Rated 5 out of 5 by from very nice qualityIt has been reported in the literature that the expression of Daxx protein in gastric cancer is very high. We used this antibody to detect the expression of Daxx. The specificity of this expression is very good. After 1:500 dilution, the target band 120kda obtained
Date published: 2017-09-16
Rated 5 out of 5 by from This antibody can get clear bands.In our lab, this antibody has good quality in Western blotting. Using 1:500 I get clear bands.
Date published: 2017-06-06
Rated 5 out of 5 by from strong signalDaxx antibody used in IF . Observed strong excellent signal.
Date published: 2017-06-03
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Daxx 抗体 (H-7) is rated 4.2 out of 5 by 21.
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