CYP7B1 抗体 (WW-H9): sc-134309

CYP7B1 Antibody (WW-H9) is an IgG_2a κ mouse monoclonal CYP7B1 antibody (also designated CYP7B1 antibody) that detects the CYP7B1 protein of human origin by WB, IP and ELISA. CYP7B1 Antibody (WW-H9) is available as the non-conjugated anti-CYP7B1 antibody. P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds including cholesterol. CYP8B1 moderates the ratio of cholic acid over chenodeoxycholic acid to control the solubility of cholesterol. P450 cholesterol 7-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver. CYP7B1 (oxysterol 7-alpha-hydroxylase) functions as an enzyme in the alternate bile acid synthesis pathway. Specifically, CYP7B1 metabolizes 25- and 27-hydroxycholesterol. The gene encoding human CYP7B1 maps to chromosome 8q21.3. Mutations in the CYP7B1 gene may cause a metabolic defect in bile acid synthesis characterized by elevated urinary bile acid excretion, severe cholestasis, cirrhosis and liver synthetic failure.

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References:

1. Disruption of the oxysterol 7alpha-hydroxylase gene in mice.  |  Li-Hawkins, J., et al. 2000. J Biol Chem. 275: 16536-42. PMID: 10748048
2. Metabolism of vitamin D(3) by human CYP27A1.  |  Sawada, N., et al. 2000. Biochem Biophys Res Commun. 273: 977-84. PMID: 10891358
3. Regulation of absorption and ABC1-mediated efflux of cholesterol by RXR heterodimers.  |  Repa, JJ., et al. 2000. Science. 289: 1524-9. PMID: 10968783
4. Molecular cloning and expression of rabbit sterol 12alpha-hydroxylase.  |  Eggertsen, G., et al. 1996. J Biol Chem. 271: 32269-75. PMID: 8943286
5. P450BM-3; a tale of two domains--or is it three?  |  Peterson, JA., et al. 1997. Steroids. 62: 117-23. PMID: 9029725
6. Identification of a new inborn error in bile acid synthesis: mutation of the oxysterol 7alpha-hydroxylase gene causes severe neonatal liver disease.  |  Setchell, KD., et al. 1998. J Clin Invest. 102: 1690-703. PMID: 9802883