Date published: 2025-9-6

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cyclin E 抗体 (E-4): sc-377100

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说明书
  • cyclin E 抗体 E-4 是小鼠单克隆 IgG2b κ,cyclin E抗体, 在129篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于rat物种的cyclin E的C-terminus的氨基酸367-396之间
  • cyclin E 抗体 (E-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 cyclin E1
  • 抗cyclin E抗体(E-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取cyclin E (E-4): sc-377100免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRPm-IgGκ BP-HRP 是 cyclin E 抗体 (E-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与cyclin E 抗体 (E-4) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

cyclin E抗体(E-4)是一种IgG2b κ小鼠单克隆cyclin E抗体(也称为CCNE1抗体),该抗体可以通过蛋白质印迹法(WB)、免疫沉淀法(IP)、免疫荧光法(IF)、免疫组织化学(IHC(P))和酶联免疫吸附测定法(ELISA)检测小鼠、大鼠和人类来源的cyclin E蛋白。cyclin E抗体(E-4)既可以作为非偶联形式的抗cyclin E抗体,也可以作为多种偶联形式的抗cyclin E抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。细胞周期蛋白最初在无脊椎动物中被鉴定为在细胞周期中剧烈振荡的蛋白质。这些蛋白质在进化过程中得到了很好的保守,并在细胞分裂的调节中发挥着至关重要的作用。基于cyclin E及其mRNA表达的循环模式,它与三种cyclin D蛋白和cyclin C一起被证明是推定的G1细胞周期蛋白,其最大水平在G1/S边界附近被检测到。已发现cyclin E以时间调节的方式与转录因子E2F相关联。细胞周期蛋白E/E2F复合物主要在细胞周期的G1阶段被检测到,并随着细胞进入S阶段而减少。已知E2F是几种S期特异性蛋白质表达的关键转录因子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cyclin E 抗体 (E-4) 参考文献:

  1. 正常和腺瘤性垂体中细胞周期蛋白D和细胞周期蛋白E的表达。  |  Jordan, S., et al. 2000. Eur J Endocrinol. 143: R1-6. PMID: 10870044
  2. 小鼠的细胞周期蛋白 E 消融。  |  Geng, Y., et al. 2003. Cell. 114: 431-43. PMID: 12941272
  3. 作为分子标记物的细胞周期蛋白 E 在乳腺癌治疗中的应用综述  |  Lopez-Beltran, A., et al. 2006. Anal Quant Cytol Histol. 28: 111-4. PMID: 16637514
  4. 细胞周期蛋白E的失调与p53的失灵相互作用:关闭乳腺癌的逃生通道。  |  Barton, MC., et al. 2006. J Cell Physiol. 209: 686-94. PMID: 17001684
  5. Cyclin E 的失调会通过过早激活 Cdc25C 而影响有丝分裂的进程。  |  Bagheri-Yarmand, R., et al. 2010. Cancer Res. 70: 5085-95. PMID: 20530684
  6. 细胞周期蛋白E转基因小鼠:肺癌生物学、治疗和预防的发现工具。  |  Freemantle, SJ. and Dmitrovsky, E. 2010. Cancer Prev Res (Phila). 3: 1513-8. PMID: 21149327
  7. Cyclin E扩增/过度表达是HER2+乳腺癌患者对曲妥珠单抗产生耐药性的机制。  |  Scaltriti, M., et al. 2011. Proc Natl Acad Sci U S A. 108: 3761-6. PMID: 21321214
  8. 细胞质细胞周期蛋白E介导乳腺癌对芳香酶抑制剂的耐药性。  |  Doostan, I., et al. 2017. Clin Cancer Res. 23: 7288-7300. PMID: 28947566
  9. USP27 介导的细胞周期蛋白 E 稳定化推动了细胞周期的进展和肝细胞肿瘤的发生。  |  Dong, L., et al. 2018. Oncogene. 37: 2702-2713. PMID: 29497124
  10. 细胞质 Cyclin E 表达可预测乳腺癌新辅助化疗的反应  |  Karakas, C., et al. 2021. Ann Surg. 274: e150-e159. PMID: 31436549

订购信息

产品名称产品编号规格价格数量收藏夹

cyclin E 抗体 (E-4)

sc-377100
200 µg/ml
$316.00

cyclin E (E-4): m-IgG Fc BP-HRP 套装

sc-529974
200 µg Ab; 10 µg BP
$354.00

cyclin E (E-4): m-IgGκ BP-HRP 套装

sc-523188
200 µg Ab, 40 µg BP
$354.00

cyclin E (E-4): m-IgG2b BP-HRP 套装

sc-550153
200 µg Ab; 10 µg BP
$354.00

cyclin E 抗体 (E-4) AC

sc-377100 AC
500 µg/ml, 25% agarose
$416.00

cyclin E 抗体 (E-4) HRP

sc-377100 HRP
200 µg/ml
$316.00

cyclin E 抗体 (E-4) FITC

sc-377100 FITC
200 µg/ml
$330.00

cyclin E 抗体 (E-4) PE

sc-377100 PE
200 µg/ml
$343.00

cyclin E 抗体 (E-4) Alexa Fluor® 488

sc-377100 AF488
200 µg/ml
$357.00

cyclin E 抗体 (E-4) Alexa Fluor® 546

sc-377100 AF546
200 µg/ml
$357.00

cyclin E 抗体 (E-4) Alexa Fluor® 594

sc-377100 AF594
200 µg/ml
$357.00

cyclin E 抗体 (E-4) Alexa Fluor® 647

sc-377100 AF647
200 µg/ml
$357.00

cyclin E 抗体 (E-4) Alexa Fluor® 680

sc-377100 AF680
200 µg/ml
$357.00

cyclin E 抗体 (E-4) Alexa Fluor® 790

sc-377100 AF790
200 µg/ml
$357.00

cyclin E (E-4) 中和勝肽

sc-377100 P
100 µg/0.5 ml
$68.00

What Li-COR/Odyssey compatible secondary is recommended for use with this cyclin E mouse primary?

Asked by: AbPolly
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-18

您好,我想问下:我这个抗体用的并不好,我想给差评可以吗?我看网站上的评论99%都是五星,我就犹豫了,差评了还能收到优惠券吗?

Asked by: xiaomao
感谢您的提问。关于如何提交评论的问题请联系我们的技术支持部门:电话(+86) 021-60936351,邮箱asia@scbio.cn。 Thank you for your question. For questions regarding reviews, please contact our Technical Services department at (+86) 021-60936351 or asia@scbio.cn.
Answered by: Technical Support 12
Date published: 2016-12-22
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Rated 1 out of 5 by from Does not workAb did not work well on neonatal lungs for Western Blot.
Date published: 2024-12-10
Rated 5 out of 5 by from good productworked well in immunohistochemistry. It's a good product.
Date published: 2022-02-15
Rated 1 out of 5 by from Not working in MCF10AWe tested siCCNE and used two santa cruz cyclin E antibodies to validate the knockdown in MCF10A cells. This one showed unchanged bands at 50kDa, whereas the other (HE-12) clearly showed the downregulation of cyclin E.
Date published: 2022-01-13
Rated 4 out of 5 by from Good antibody for cycEWe used this antibody to detect cycE expressed from a doxycyclin-inducible plasmid in human MCF7 and T47D cells by Western blot and it worked perfectly well, with a strong and specific band.
Date published: 2021-11-19
Rated 5 out of 5 by from 产品很好我10月份订购的此抗体,实验室之前的货号停产更换为此货号,在试验后 条带清晰,效果明显,做细胞增殖需要的朋友,很推荐此款抗体!
Date published: 2018-11-26
Rated 5 out of 5 by from ExcellentThe antibody was used to examine the protein of cyclin E in rat tissues. It is excellent. The bands were clear and specific.
Date published: 2018-11-14
Rated 5 out of 5 by from Great product!!It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 1 out of 5 by from Unspecific bandsWe used this antibody in WB, and the bands we got were not at the expected size!
Date published: 2018-03-15
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cyclin E 抗体 (E-4) is rated 4.2 out of 5 by 38.
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