Date published: 2025-9-7
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CtIP Antibody (D-4) - Western Blotting - Image 296902
CtIP 抗体 (D-4): sc-271339. T24 (A) 和 F9 (B) 全细胞裂解液中 CtIP 表达的 Western 印迹分析.
CtIP 抗体 (D-4): sc-271339. T24 (A) 和 F9 (B) 全细胞裂解液中 CtIP 表达的 Western 印迹分析.

CtIP 抗体 (D-4): sc-271339

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寫評論提問

说明书
  • CtIP 抗体 D-4 是小鼠单克隆 IgG2b κ，CtIP抗体, 在49篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的CtIP的C-terminus附近的氨基酸835-865之间
  • CtIP 抗体 (D-4) 推荐用于WB, IP, IF 和 ELISA ，检测 mouse, rat, human 和 来源的CtIP; 也和以下物种反应，包括： canine and avian
  • 抗CtIP抗体（D-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CtIP (D-4): sc-271339的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是CtIP Antibody (D-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CtIP Antibody (D-4) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CtIP抗体（D-4）是检测小鼠CtIP蛋白的IgG2bκ小鼠单克隆CtIP单克隆抗体（也称为RB结合蛋白8抗体、视网膜母细胞瘤结合蛋白8抗原、CTBP相互作用蛋白抗体、DNA内切酶RBBP8抗体、RBBP-8抗体、COM1抗体、SAE2抗体或视网膜母细胞癌相互作用蛋白和肌球蛋白样抗体），通过WB、IP、IF和ELISA检测大鼠和人来源的CtIP。CtIP抗体（D-4）既有非偶联的抗CtIP的抗体形式，也有多种偶联的抗-CtIP抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。CtBP1是一种细胞磷酸蛋白，可与多种蛋白质相互作用，并充当转录抑制因子。CtBP1和相关蛋白CtBP2被认为是腺病毒E1A的C末端特异性结合蛋白，它们通过一个五氨基酸基序PLDLS优先与E1A结合，以抑制E1A诱导的致癌和细胞转化。CtBP1从胚胎到成人都有表达，而CtBP2主要在胚胎发育期表达。在骨骼和T细胞发育期，CtBP1和CtBP2与dEF1的PLDLSL结构域结合，dEF1是一种细胞锌指-家族蛋白，从而增强dEF1诱导的转录抑制。此外，CtBP与CtIP结合，CtIP是一种与CtBP不同的蛋白质基序识别蛋白。CtIP与乳腺癌基因BRCA1的BRCT重复结合，使CtBP能够影响BRCA1活性。CtIP/CtBP与BRCA1的结合抑制p21启动子的转录激活，对于调节DNA损伤反应中p21转录起着关键作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CtIP 抗体 (D-4) 参考文献:

  1. CtIP 与参与 p21 转录调控的 BRCA1 的 BRCT 重复序列的结合在 DNA 损伤时被破坏。  |  Li, S., et al. 1999. J Biol Chem. 274: 11334-8. PMID: 10196224
  2. 确定 CtBP1 和 CtBP2 为锌指-homeodomain 因子 deltaEF1 的核心加压因子。  |  Furusawa, T., et al. 1999. Mol Cell Biol. 19: 8581-90. PMID: 10567582
  3. 抗肿瘤细胞溶解 T 细胞的出现与急性髓性白血病患儿血液学缓解的维持缔合作用。  |  Montagna, D., et al. 2006. Blood. 108: 3843-50. PMID: 16868250
  4. 在宫颈癌细胞中，miR-130b会削弱CTIP介导的TNF-α诱导的DNA双链断裂的修复。  |  Yang, L., et al. 2019. Cell Biochem Funct. 37: 534-544. PMID: 31418900
  5. SUMO化介导CtIP在DNA末端切除和复制叉保护中的作用。  |  Locke, AJ., et al. 2021. Nucleic Acids Res. 49: 928-953. PMID: 33406258
  6. DNA断裂侧翼依赖于CtIP的新生RNA表达引导着DNA修复途径的选择。  |  Gómez-Cabello, D., et al. 2022. Nat Commun. 13: 5303. PMID: 36085345
  7. 腺病毒 E1A 蛋白中的 CtBP 结合域控制着 CR1 依赖性反式激活。  |  Sollerbrant, K., et al. 1996. Nucleic Acids Res. 24: 2578-84. PMID: 8692699
  8. 一种与腺病毒 E1A（CtBP）C 端区域结合的细胞蛋白与一种新型细胞蛋白之间的相互作用被 E1A 通过一个保守的 PLDLS 基序破坏。  |  Schaeper, U., et al. 1998. J Biol Chem. 273: 8549-52. PMID: 9535825
  9. 抑制剂 deltaEF1 的两种作用机制：与激活剂竞争结合位点和主动抑制。  |  Sekido, R., et al. 1997. Genes Cells. 2: 771-83. PMID: 9544704
  10. mCtBP2 的克隆和特征描述，mCtBP2 是一种与碱性克鲁珀尔样因子及其他哺乳动物转录调节因子相关联的共抑制因子。  |  Turner, J. and Crossley, M. 1998. EMBO J. 17: 5129-40. PMID: 9724649
  11. BRCA1 的 C 端（BRCT）结构域在体内与 CtIP 相互作用，CtIP 是一种参与 CtBP 转录抑制途径的蛋白质。  |  Yu, X., et al. 1998. J Biol Chem. 273: 25388-92. PMID: 9738006
  12. 羧基末端 BRCA1 交互蛋白的特征。  |  Wong, AK., et al. 1998. Oncogene. 17: 2279-85. PMID: 9811458

订购信息

产品名称产品编号规格价格数量收藏夹

CtIP 抗体 (D-4)

sc-271339
200 µg/ml
$316.00

CtIP (D-4): m-IgGκ BP-HRP 套装

sc-521916
200 µg Ab, 40 µg BP
$354.00

CtIP (D-4): m-IgG2b BP-HRP 套装

sc-548984
200 µg Ab; 10 µg BP
$354.00

CtIP 抗体 (D-4) AC

sc-271339 AC
500 µg/ml, 25% agarose
$416.00

CtIP 抗体 (D-4) HRP

sc-271339 HRP
200 µg/ml
$316.00

CtIP 抗体 (D-4) FITC

sc-271339 FITC
200 µg/ml
$330.00

CtIP 抗体 (D-4) PE

sc-271339 PE
200 µg/ml
$343.00

CtIP 抗体 (D-4) Alexa Fluor® 488

sc-271339 AF488
200 µg/ml
$357.00

CtIP 抗体 (D-4) Alexa Fluor® 546

sc-271339 AF546
200 µg/ml
$357.00

CtIP 抗体 (D-4) Alexa Fluor® 594

sc-271339 AF594
200 µg/ml
$357.00

CtIP 抗体 (D-4) Alexa Fluor® 647

sc-271339 AF647
200 µg/ml
$357.00

CtIP 抗体 (D-4) Alexa Fluor® 680

sc-271339 AF680
200 µg/ml
$357.00

CtIP 抗体 (D-4) Alexa Fluor® 790

sc-271339 AF790
200 µg/ml
$357.00

CtIP (D-4) 中和勝肽

sc-271339 P
100 µg/0.5 ml
$68.00

Can CtIP (D-4): sc-271339 be frozen?

Asked by: jenniferc
No. The antibody solution contains gelatin. When the gelatin is frozen the antibody precipitates out of solution. However, if an antibody’s datasheet says that the antibody should be stored frozen, then the antibody can be frozen, as these antibodies do not contain gelatin.
Answered by: Technical Support 15
Date published: 2017-01-24

What is the recommended secondary reagent for detection of the CtIP Antibody (D-4) in Western blot applications?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-19

I see the CtIP Antibody (D-4) has reactivity with multiple species. Will it also work with porcine samples?

Asked by: Trav11
To inquire about additional species reactivity, please contact our Technical Services department and we will be more than happy to run sequence analysis for the species of interest.
Answered by: Tech Service 8
Date published: 2016-12-19
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Rated 3 out of 5 by from Good antibodyAntibody works for western blots using 30ug of protein
Date published: 2024-04-05
Rated 2 out of 5 by from Doesn't work very wellI did not see clear band by western blot (1:1000 dilution) and not a clear signal by immunofluorescence (1:100) in ONS-76 cells
Date published: 2021-11-26
Rated 4 out of 5 by from Works well for western blottingWe used this antibody for both western blotting and IF assays. It worked well for western blotting, can also be detected in IF at 1:30.
Date published: 2017-12-27
Rated 1 out of 5 by from Did not workTried a sample of this antibody with mouse hybridoma cells. Absolutely no signal at all detected. Tried overnight incubation and tried both 50ug and 100ug of total cell protein.
Date published: 2017-09-08
Rated 5 out of 5 by from Works well with IF on mouse spermatocytesVery clean IF on mouse pachytene spermatocytes at 1:100 dilution.
Date published: 2017-06-21
Rated 5 out of 5 by from same epitope as polyclonal sc-5970This new mouse monoclonal antibody (D-4) clone is raised against the same C-terminal peptide used to develop the now discontinued polyclonal antibody, sc-5970. This mono is an excellent choice overall and a suitable replacement.
Date published: 2016-12-29
Rated 5 out of 5 by from well done in human lung cancer cell linesThe antibody is worked well by the dilution of 1:150 in human lung cancer cell lyaste.
Date published: 2016-10-27
Rated 5 out of 5 by from Performed well in WB in T24 and F9 wholePerformed well in WB in T24 and F9 whole cell lysates. -SCBT QC
Date published: 2015-06-12
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CtIP 抗体 (D-4) is rated 4.2 out of 5 by 13.
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