Date published: 2025-9-6

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CLASP2 抗体 (F-3): sc-376496

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说明书
  • CLASP2 抗体 F-3 是小鼠单克隆 IgG1 κ,CLASP2抗体, 在3篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列21-60位于human物种的CLASP2的N-terminus附近
  • CLASP2 抗体 (F-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 CLASP2
  • 抗CLASP2抗体(F-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CLASP2 (F-3): sc-376496免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是CLASP2 Antibody (F-3) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与CLASP2 Antibody (F-3) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

CLASP2抗体(F-3)是一种IgG1κ小鼠单克隆CLASP2抗体(也称为CLASP2抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的CLASP2蛋白。CLASP2抗体(F-3)可以是非偶联的抗CLASP2抗体形式,也可以是多种偶联形式的抗CLASP2抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。CLASP2(细胞质连接蛋白2),也称为KIAA0627或hOrbit2,是一种包含五个HEAT重复序列的1294个氨基酸的蛋白质,定位于细胞质和细胞骨架,以及动粒和高尔基体。CLASP2主要在脑组织中表达,作为微管正端追踪蛋白,调节动态微管的稳定性,并且是迁移细胞中细胞质微管阵列正确极化所必需的。CLASP2与EB1、EB3、ELKS和CLIP-115相互作用,除了稳定微管外,还在维持动粒的稳定性方面发挥重要作用,并且对于正确的染色体排列至关重要。CLASP2受GSK-3β的磷酸化调节,这一事件被认为会负向调节CLASP2与微管结合的能力。由于可选择性剪接事件,CLASP2存在两种亚型,分别称为β和γ。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CLASP2 抗体 (F-3) 参考文献:

  1. Clasps是CLIP-115和-170缔合作用蛋白,参与运动成纤维细胞微管动力学的区域调控。  |  Akhmanova, A., et al. 2001. Cell. 104: 923-35. PMID: 11290329
  2. 微管末端追踪蛋白Orbit/MAST/CLASP在酪氨酸激酶Abl的下游发挥作用,介导轴突导向。  |  Lee, H., et al. 2004. Neuron. 42: 913-26. PMID: 15207236
  3. CLASP1 和 CLASP2 与 EB1 结合,调节细胞皮层的微管加端动态。  |  Mimori-Kiyosue, Y., et al. 2005. J Cell Biol. 168: 141-53. PMID: 15631994
  4. CLIPs 和 CLASPs 与细胞动力学。  |  Galjart, N. 2005. Nat Rev Mol Cell Biol. 6: 487-98. PMID: 15928712
  5. 迁移上皮细胞中 Rac1 和 GSK3beta 对 CLASP 与微管亲和力的空间调控。  |  Wittmann, T. and Waterman-Storer, CM. 2005. J Cell Biol. 169: 929-39. PMID: 15955847
  6. CLASPs 通过与 LL5beta 复合物将微管加端连接到细胞皮层。  |  Lansbergen, G., et al. 2006. Dev Cell. 11: 21-32. PMID: 16824950
  7. 哺乳动物的CLASPs是有丝分裂纺锤体组织和着丝点排列所必需的。  |  Mimori-Kiyosue, Y., et al. 2006. Genes Cells. 11: 845-57. PMID: 16866869
  8. 哺乳动物的 CLASP1 和 CLASP2 相互合作,通过调节纺锤体和着丝点的功能来确保有丝分裂的保真度。  |  Pereira, AL., et al. 2006. Mol Biol Cell. 17: 4526-42. PMID: 16914514

订购信息

产品名称产品编号规格价格数量收藏夹

CLASP2 抗体 (F-3)

sc-376496
200 µg/ml
$316.00

CLASP2 (F-3): m-IgG Fc BP-HRP 套装

sc-529882
200 µg Ab; 10 µg BP
$354.00

CLASP2 (F-3): m-IgGκ BP-HRP 套装

sc-522975
200 µg Ab, 40 µg BP
$354.00

CLASP2 (F-3): m-IgG1 BP-HRP 套装

sc-543741
200 µg Ab; 20 µg BP
$354.00

CLASP2 抗体 (F-3) AC

sc-376496 AC
500 µg/ml, 25% agarose
$416.00

CLASP2 抗体 (F-3) HRP

sc-376496 HRP
200 µg/ml
$316.00

CLASP2 抗体 (F-3) FITC

sc-376496 FITC
200 µg/ml
$330.00

CLASP2 抗体 (F-3) PE

sc-376496 PE
200 µg/ml
$343.00

CLASP2 抗体 (F-3) Alexa Fluor® 488

sc-376496 AF488
200 µg/ml
$357.00

CLASP2 抗体 (F-3) Alexa Fluor® 546

sc-376496 AF546
200 µg/ml
$357.00

CLASP2 抗体 (F-3) Alexa Fluor® 594

sc-376496 AF594
200 µg/ml
$357.00

CLASP2 抗体 (F-3) Alexa Fluor® 647

sc-376496 AF647
200 µg/ml
$357.00

CLASP2 抗体 (F-3) Alexa Fluor® 680

sc-376496 AF680
200 µg/ml
$357.00

CLASP2 抗体 (F-3) Alexa Fluor® 790

sc-376496 AF790
200 µg/ml
$357.00

did you have any feedback on the use of this AB in IF ?

Asked by: AS UGSF
Thank you for your question. sc-376496 is recommended to be used for the detection of CLASP2 by WB, IP, IF, IHC(P) and ELISA.
Answered by: Tech Support Europe
Date published: 2022-03-09

您好,我买了这个抗体,最近在用大鼠骨骼肌冰冻切片做免疫荧光,但做出来结果不理想,您能给我一个具体的实验步骤吗?谢谢!

Asked by: 秋中梅林
感谢您的提问。免疫荧光的实验步骤,请参考链接: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining 。Thank you for your question. Please refer to this link for the protocol of IF: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining .
Answered by: Technical Support 8
Date published: 2018-12-21

May I ask if your company can produce anti-phosphorylation CLASP2 antibody?If so, how long would it take?

Asked by: tianmei Li
Thank you for your question. We do not currently offer any phospho-specific antibodies against the CLASP2 protein. Please check back later to see if we have updated our catalog. However, we do not offer any custom antibody production services.
Answered by: Technical Support
Date published: 2018-12-21

Can CLASP2 (F-3): sc-376496 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, CLASP2 (F-3): sc-376496 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from good productThe signal for CLASP2 in U2OS cell line is good with 1:1000 dilution .
Date published: 2018-02-09
Rated 5 out of 5 by from WB publishable data with human HCT116 andWB publishable data with human HCT116 and isogenic CHK2-deficient cells - SCBT Publication Review
Date published: 2015-04-06
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic stainingExcellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human cerebral cortex tissue. -SCBT QC
Date published: 2014-10-14
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CLASP2 抗体 (F-3) is rated 5.0 out of 5 by 3.
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