Date published: 2025-9-7
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Chx10 Antibody (E-12) - Immunohistochemistry - Image 369571
Chx10 抗体 (E-12): sc-365519. 福尔马林固定, 石蜡包埋的人胎儿眼组织的免疫过氧化物酶染色显示视网膜内细胞层的核染色.
Chx10 抗体 (E-12): sc-365519. 福尔马林固定, 石蜡包埋的人胎儿眼组织的免疫过氧化物酶染色显示视网膜内细胞层的核染色.

Chx10 抗体 (E-12): sc-365519

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说明书
  • Chx10 抗体 E-12 是小鼠单克隆 IgG2a κ，Chx10抗体, 在31篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Chx10的N-terminus附近的氨基酸37-64之间
  • Chx10 抗体 (E-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Chx10; 也和以下物种反应，包括： 和 equine, canine and porcine
  • 可用于ChIP的TransCruz试剂(sc-365519 X, 200 µg/0.1 ml)
  • 抗Chx10抗体（E-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Chx10 (E-12): sc-365519的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 Chx10 抗体 (E-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Chx10 抗体 (E-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Chx 抗体

Chx10抗体（E-12）是一种IgG2a κ小鼠单克隆Chx10抗体（也被称为视觉系统同源盒2抗体、含有Ceh-10同源结构域的同源抗体、同源盒蛋白CHX10抗体、HOX10抗体、RET1抗体、含有Ceh-10同源结构域的同源物（C.线虫）抗体、C线虫Ceh-10同源结构域含有同源物抗体、MCOPCB3抗体或MCOP2抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的Chx10蛋白。Chx10抗体（E-12）既有非偶联的抗Chx10抗体形式，也有多种偶联形式的抗Chx10抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Chx10，即含有ceh-10同源结构域的基因，也被称为RET1和HOX10，与线虫秀丽隐杆线虫的同源盒基因ceh-10的同源结构域密切相关。Chx10是哺乳动物眼睛发育所需基因网络中的一个重要组成部分，对视网膜祖细胞的增殖以及双极细胞的特化或分化具有深远影响。Chx10在早期视网膜神经上皮中表达，仅限于双极细胞，并在成熟视网膜的双极细胞中维持低水平表达。人类CHX10也在成熟视网膜的内核层中表达。表达模式表明了Chx10在神经视网膜的形成以及内核层的发育和维持中的关键作用。Chx10在未分化的视网膜祖细胞和成熟的双极细胞中高水平表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Chx10 抗体 (E-12) 参考文献:

  1. Chx10 和 Chx10-1 在发育中的鸡视网膜中的表达。  |  Chen, CM. and Cepko, CL. 2000. Mech Dev. 90: 293-7. PMID: 10640715
  2. 与视网膜同源基因 CHX10 突变有关的人类小眼症。  |  Ferda Percin, E., et al. 2000. Nat Genet. 25: 397-401. PMID: 10932181
  3. Chx10对Mitf的抑制作用对于维持哺乳动物神经视网膜的特性是必需的。  |  Horsford, DJ., et al. 2005. Development. 132: 177-87. PMID: 15576400
  4. CHX10靶向一组光感受器基因。  |  Dorval, KM., et al. 2006. J Biol Chem. 281: 744-51. PMID: 16236706
  5. 缺乏chx10会导致神经祖细胞在成年视网膜中持续存在。  |  Dhomen, NS., et al. 2006. Invest Ophthalmol Vis Sci. 47: 386-96. PMID: 16384989
  6. Chx10是阻止感光细胞分化的必要条件，但对出生后视网膜中的祖细胞增殖则不是必需的。  |  Livne-Bar, I., et al. 2006. Proc Natl Acad Sci U S A. 103: 4988-93. PMID: 16547132
  7. 由表达Chx10的神经元组成的脑干固有回路有助于小鼠的网状脊髓输出。  |  Chopek, JW., et al. 2021. J Neurophysiol. 126: 1978-1990. PMID: 34669520
  8. 脊髓运动调节和脊髓损伤中的Chx10+V2a中间神经元。  |  Li, WY., et al. 2023. Neural Regen Res. 18: 933-939. PMID: 36254971
  9. Pedunculopontine Chx10+神经元控制小鼠的整体运动停止。  |  Goñi-Erro, H., et al. 2023. Nat Neurosci. 26: 1516-1528. PMID: 37501003
  10. elegans 神经元表达的同源染色体基因 ceh-10 与脊椎动物眼睛中表达的基因密切相关。  |  Svendsen, PC. and McGhee, JD. 1995. Development. 121: 1253-62. PMID: 7789259
  11. 一种新型小鼠同源染色体基因（Chx10）的发育表达：决定神经视网膜和核内层作用的证据。  |  Liu, IS., et al. 1994. Neuron. 13: 377-93. PMID: 7914735
  12. Chx10同源染色体空等位基因导致的眼球发育迟缓小鼠：视网膜祖细胞增殖和双极细胞分化受损  |  Burmeister, M., et al. 1996. Nat Genet. 12: 376-84. PMID: 8630490

订购信息

产品名称产品编号规格价格数量收藏夹

Chx10 抗体 (E-12)

sc-365519
200 µg/ml
$316.00

Chx10 (E-12): m-IgG Fc BP-HRP 套装

sc-525887
200 µg Ab; 10 µg BP
$354.00

Chx10 (E-12): m-IgG2a BP-HRP 套装

sc-546453
200 µg Ab; 10 µg BP
$354.00

Chx10 抗体 (E-12) X

sc-365519 X
200 µg/0.1 ml
$316.00

Chx10 抗体 (E-12) AC

sc-365519 AC
500 µg/ml, 25% agarose
$416.00

Chx10 抗体 (E-12) HRP

sc-365519 HRP
200 µg/ml
$316.00

Chx10 抗体 (E-12) FITC

sc-365519 FITC
200 µg/ml
$330.00

Chx10 抗体 (E-12) PE

sc-365519 PE
200 µg/ml
$343.00

Chx10 抗体 (E-12) Alexa Fluor® 488

sc-365519 AF488
200 µg/ml
$357.00

Chx10 抗体 (E-12) Alexa Fluor® 546

sc-365519 AF546
200 µg/ml
$357.00

Chx10 抗体 (E-12) Alexa Fluor® 594

sc-365519 AF594
200 µg/ml
$357.00

Chx10 抗体 (E-12) Alexa Fluor® 647

sc-365519 AF647
200 µg/ml
$357.00

Chx10 抗体 (E-12) Alexa Fluor® 680

sc-365519 AF680
200 µg/ml
$357.00

Chx10 抗体 (E-12) Alexa Fluor® 790

sc-365519 AF790
200 µg/ml
$357.00

Chx10 (E-12) 中和勝肽

sc-365519 P
100 µg/0.5 ml
$68.00

Did anyone use this antibody for immunofluoresence staining on the human cells before? if yes, may I please get the concentration and the immunostaining condition from you? 

Asked by: Phuong Lam
Thank you for the question. We suggest a starting dilution of 1:50 for staining methanol-fixed human cells and suggest following our corresponding protocols: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining You might also want to check the following citation regarding the use of this antibody: https://www.ncbi.nlm.nih.gov/pubmed/24327609?dopt=Abstract
Answered by: Technical Support Europe
Date published: 2018-01-09

What application is the blocking peptide sc-365519 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from IF detection PFA-fixed cryosectionsSample: healthy adult Rhesus macaque retina Fix: 4% PFA 2hrs @RT HIER: 5min microwave retrieval in Tris-EDTA (pH 9.0) Block: 10% serum in PBS +0.1% triton for 1 hour Dilution: 1:150 in 10% serum in 1X PBS Primary Incubation: 15hrs @4C Secondary: Mouse polyclonal, Alexa Fluor 488 (Invitrogen A-21202)
Date published: 2020-02-06
Rated 5 out of 5 by from Specific and reliable antibody!Great antibody! Currently using in human IPSC-derived retinal ganglion cells and in adult rat retina (for positive control). Ms-CHX10 used @ 1:200 in blocking solution (5%NGS, 0.4% BSA, 0.1% TX-100) overnight @ 4 degree. Secondary antibody used @ 1:300.
Date published: 2019-10-08
Rated 5 out of 5 by from Works well on pig retinaTested on pig frozen retina 8um sections (IHC-Fr). Blocking: 5% donkey serum; P Ab: 1:50 (overnight); S Ab: anti-mouse 1:200 (1h)
Date published: 2017-10-23
Rated 4 out of 5 by from good when tried twiceI use this antibody to detect the expression of Chx10 of Mesenchymal stem cells after certain neural induction . The following procedures were conducted with WB 1The cells were lysated with RIPA, PMSF, and proteinase cocktail ,then ultrasound 2 electrophoresis, transfer to NC membrane 3 incubated with first antibody sc-365519)is diluted at:1:150 ,4 °C,overnight (first time at 1:250， failed) 4 washed, incubated with goat anti-mouse HRP conjugate second antibody, ECL coloration 5 results good anyway ,the protein band is not bad
Date published: 2017-01-19
Rated 4 out of 5 by from Funciona muy bienHice una prueba con este producto en inmunofluorescencia en retina de ratón adulto y marca perfectamente la población celular de interés.
Date published: 2016-12-19
Rated 5 out of 5 by from Satisfactory Western Blot data of Chx10 expressionSatisfactory Western Blot data of Chx10 expression in mouse eye tissue extract. -SCBT QC
Date published: 2015-02-06
Rated 5 out of 5 by from Good Western Blot data of Chx10 expression in nonGood Western Blot data of Chx10 expression in non-transfected and mouse Chx10 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-11-29
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Chx10 抗体 (E-12) is rated 4.7 out of 5 by 7.
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