Date published: 2026-10-22
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Chk2 Antibody (DCS-270) - Western Blotting - Image 354015
Chk2 抗体 (DCS-270): sc-56296. CCRF-CEM (A), K-562 (B), Jurkat (C), MCF7 (D) 和 HeLa (E) 全细胞裂解液中 Chk2 表达的 Western 印迹分析.
Chk2 抗体 (DCS-270): sc-56296. CCRF-CEM (A), K-562 (B), Jurkat (C), MCF7 (D) 和 HeLa (E) 全细胞裂解液中 Chk2 表达的 Western 印迹分析.

Chk2 抗体 (DCS-270): sc-56296

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寫評論提問
说明书
  • Chk2抗体(DCS-270)是小鼠单克隆IgG2a κ, 在14篇文献中引用，规格为200 µg/ml
  • 针对Chk2融合蛋白的抗体，来源于human
  • 推荐用于 human 来源的Chk2 WB, IP, IF 和 IHC(P)检测
  • 看 Chk2 (A-11): sc-17747，可查看有标记的 Chk2 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是Chk2 Antibody (DCS-270) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Chk2 Antibody (DCS-270) 打包提供（请参阅下面的订购信息）。
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研究信息

関連項目

额外Chk抗体，包括Chk2

细胞周期事件受到细胞周期蛋白依赖性激酶（Cdks）的顺序激活和失活以及细胞周期蛋白的水解调节，而Chk1和Chk2作为Cdks的调节因子参与这些过程。特别地，Chk1和Chk2在G2 DNA损伤检查点中发挥着至关重要的作用，它们通过响应DNA损伤来磷酸化Cdc25C。这种磷酸化作用抑制了Cdc25C的活性，从而阻止了细胞进入有丝分裂阶段。此外，Cdc25A、Cdc25B和Cdc25C蛋白酪氨酸磷酸酶则通过去磷酸化调节性酪氨酸残基上的Cdc2 p34来发挥有丝分裂激活剂的作用。值得一提的是，研究还表明Chk1可以在体外磷酸化Wee1，这为进一步证实细胞内由于Chk1过度表达而导致的Wee1超磷酸化状态提供了证据，这种状态是由Chk1对Wee1的磷酸化作用所引起的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Chk2 抗体 (DCS-270) 参考文献:

  1. CHK1和CHK2在维持基因组稳定性中的多重检查点功能。  |  Chen, Y. and Poon, RY. 2008. Front Biosci. 13: 5016-29. PMID: 18508566
  2. CHK2 的稳定性受 E3 泛素连接酶 SIAH2 的调控。  |  García-Limones, C., et al. 2016. Oncogene. 35: 4289-301. PMID: 26751770
  3. 缺乏 Chk2 会抑制 p53 依赖性细胞凋亡，从而缓解辐照诱导的味觉功能障碍。  |  Yuan, Z., et al. 2018. Oral Dis. 24: 856-863. PMID: 29292572
  4. CHK2促进细胞凋亡，与甲状腺乳头状癌的进展有关。  |  Zhao, W., et al. 2018. Cell Physiol Biochem. 45: 1590-1602. PMID: 29486482
  5. 癌症和癌症干细胞中的 ATM, ATR, CHK1, CHK2 和 WEE1 抑制剂。  |  Ronco, C., et al. 2017. Medchemcomm. 8: 295-319. PMID: 30108746
  6. SIRT1 通过调节 CHK2 的乙酰化-磷酸化来调节细胞周期的进展。  |  Zhang, W., et al. 2020. Cell Death Differ. 27: 482-496. PMID: 31209362
  7. Chk2依赖性肌球蛋白磷酸酶靶向亚基1（MYPT1）磷酸化调控中心体成熟。  |  Nai, S., et al. 2019. Cell Cycle. 18: 2651-2659. PMID: 31416392
  8. AR磷酸化和CHK2激酶活性调节IR稳定的AR-CHK2相互作用和前列腺癌的存活。  |  Ta, HQ., et al. 2020. Elife. 9: PMID: 32579110
  9. Chk2-PKM2轴促进p53突变的三阴性乳腺癌中血管生成模拟形成的代谢控制。  |  Yu, P., et al. 2021. Oncogene. 40: 5262-5274. PMID: 34244606
  10. 纳米粒子介导的PRDX2抑制特异性靶向CHK2缺失的结直肠癌  |  Ahmad, A., et al. 2022. ACS Biomater Sci Eng. 8: 5210-5220. PMID: 36446128

订购信息

产品名称产品编号规格价格数量收藏夹

Chk2 抗体 (DCS-270)

sc-56296
200 µg/ml
$322.00

Chk2 (DCS-270): m-IgGκ BP-HRP 套装

sc-553182
200 µg Ab; 40 µg BP
$361.00

Chk2 (DCS-270): m-IgG2a BP-HRP 套装

sc-553183
200 µg Ab; 10 µg BP
$361.00

I am using Chk2 (DCS-270): sc-56296 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by jenniferc from Great monoclonal antibodyNice robust IHCP stain and strong specific band detected by WB with little to no background
Date published: 2017-02-06
Rated 5 out of 5 by ET from Strong pulldown in IP in human UStrong pulldown in IP in human U-2-OS and transformed ATM-deficient cells. -SCBT Publication Review
Date published: 2015-05-02
Rated 5 out of 5 by QZ from Produced great immunoperoxidase nuclear stainingProduced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human colon tissue. -SCBT QC
Date published: 2015-04-24
Rated 5 out of 5 by LR from Good publishable data in WB in human HCT116Good publishable data in WB in human HCT116, TO53, HCC1937. -SCBT Publication Review
Date published: 2015-01-18
Rated 5 out of 5 by MS from Strong band in WB in HeLa whole cell lysateStrong band in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-17
Rated 5 out of 5 by JM from Publishable Western blot results using 1:800Publishable Western blot results using 1:800. -SCBT Publication Review
Date published: 2014-06-29
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Chk2 抗体 (DCS-270) is rated 5.0 out of 5 by 6.
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