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Chitotriosidase 抗体 (A-11): sc-271460

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说明书
  • Chitotriosidase 抗体 A-11 是小鼠单克隆 IgM κ,Chitotriosidase抗体, 在2篇文献中引用,规格为200 µg/ml
  • 针对human起源的Chitotriosidase内部区域内的氨基酸150-215进行
  • 推荐用于 mouse, rat 和 human 来源的Chitotriosidase WB, IP, IF 和 ELISA检测
  • 关于如何获取Chitotriosidase (A-11): sc-271460免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是Chitotriosidase Antibody (A-11) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与Chitotriosidase Antibody (A-11) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

Chitotriosidase抗体(A-11)是一种IgM κ小鼠单克隆Chitotriosidase抗体(也称为CHIT1抗体、Chitotriosidase-1抗体或几丁质酶-1抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Chitotriosidase蛋白。 Chitotriosidase抗体(A-11)可作为非偶联抗Chitotriosidase抗体。几丁质酶是普遍存在的几丁质片段水解酶。几丁质酶Chitotriosidase能够切割人体内天然的几丁质和几丁质样底物,可能在抵抗含几丁质的病原体的免疫力中发挥作用。活化的人巨噬细胞分泌Chitotriosidase,血浆中Chitotriosidase水平升高是戈谢病患者的特征。小鼠Chitotriosidase的表达仅限于脑、皮肤、骨髓、肾、舌、胃和睾丸。低等生物中发现的Chitotriosidase和几丁质酶之间存在显著同源性。酸性哺乳动物几丁质酶前体(AMCase)降解三糖和几丁质。AMCase在胃组织中高度表达,主要是一种分泌蛋白。它参与Th2介导的炎症,可能在哮喘和过敏性疾病中发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Chitotriosidase 抗体 (A-11) 参考文献:

  1. 酸性哺乳动物几丁质酶在哮喘 Th2 炎症和 IL-13 途径激活中的作用  |  Zhu, Z., et al. 2004. Science. 304: 1678-82. PMID: 15192232
  2. 血浆壳三糖苷酶和 CCL18:B 型尼曼-皮克病的早期生化替代标记物。  |  Brinkman, J., et al. 2005. J Inherit Metab Dis. 28: 13-20. PMID: 15702402
  3. 戈谢病和其他溶酶体贮积疾病生物标志物的鉴定和使用。  |  Aerts, JM., et al. 2005. Acta Paediatr Suppl. 94: 43-6; discussion 37-8. PMID: 15895711
  4. 戈谢病生物标志物的临床评估。  |  Deegan, PB. and Cox, TM. 2005. Acta Paediatr Suppl. 94: 47-50; discussion 37-8. PMID: 15895712
  5. γ干扰素, 白细胞介素-10, 脂多糖和肿瘤坏死因子-α对人巨噬细胞中壳三糖苷酶合成的影响。  |  Di Rosa, M., et al. 2005. Clin Chem Lab Med. 43: 499-502. PMID: 15899671
  6. γ干扰素, 肿瘤坏死因子-α和脂多糖促进人巨噬细胞中壳三糖苷酶基因的表达。  |  Malaguarnera, L., et al. 2005. J Clin Lab Anal. 19: 128-32. PMID: 15900564
  7. 小鼠和人类几丁质酶的组织特异性表达存在明显差异。  |  Boot, RG., et al. 2005. J Histochem Cytochem. 53: 1283-92. PMID: 15923370
  8. 重组人酸性哺乳动物几丁质酶的动力学特征。  |  Chou, YT., et al. 2006. Biochemistry. 45: 4444-54. PMID: 16584180
  9. 急性冠状动脉综合征中的血清壳三苷酶活性。  |  Yildiz, BS., et al. 2013. J Atheroscler Thromb. 20: 134-41. PMID: 22986554
  10. 晚期心力衰竭门诊患者体内壳三糖苷酶的临床意义  |  Cetin Sanlialp, S., et al. 2021. J Investig Med. 69: 736-741. PMID: 33272933
  11. 作为早期肌萎缩性脊髓侧索硬化症生物标志物的壳三糖苷酶:一项多中心研究。  |  Steinacker, P., et al. 2021. Amyotroph Lateral Scler Frontotemporal Degener. 22: 276-286. PMID: 33576252
  12. 血清和支气管肺泡灌洗液壳三糖苷酶活性对肉样瘤病的诊断、疾病特征和预后的作用。  |  Köycü Buhari, G., et al. 2023. Tuberk Toraks. 71: 367-377. PMID: 38152007

订购信息

产品名称产品编号规格价格数量收藏夹

Chitotriosidase 抗体 (A-11)

sc-271460
200 µg/ml
$316.00

Chitotriosidase (A-11): m-IgGκ BP-HRP 套装

sc-521949
200 µg Ab, 40 µg BP
$354.00

Can this antibody be use for tissue IHC? If so what is the recommended starting dilution?

Asked by: Jyap99
Thank you for your question. We have not yet tested this mouse monoclonal IgM, Chitotriosidase Antibody (A-11): sc-271460, on formalin-fixed paraffin-embedded tissue sections so IHC(P) is not a recommended application at this time. We do recommend this antibody for IF, or immunofluorescent staining of methanol fixed cells, or acetone fixed fresh or frozen tissue sections.
Answered by: Technical Support
Date published: 2017-04-13

What is the recommended fixation method for immunofluorescence staining with Chitotriosidase (A-11): sc-271460 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Produced exceptional immunofluorescence cytoplasmicProduced exceptional immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-01
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Chitotriosidase 抗体 (A-11) is rated 5.0 out of 5 by 1.
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