Date published: 2025-9-8
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cGAS Antibody (D-9) - Western Blotting - Image 394848
cGAS 抗体 (D-9) Alexa Fluor® 647: sc-515777 AF647. 直接荧光西部印迹分析 T24 全细胞裂解液中 cGAS 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214.
cGAS 抗体 (D-9) Alexa Fluor® 647: sc-515777 AF647. 直接荧光西部印迹分析 T24 全细胞裂解液中 cGAS 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214.

cGAS 抗体 (D-9): sc-515777

3.9(14)
寫評論提問

说明书
  • cGAS抗体(D-9)是小鼠单克隆IgG2a κ, 在44篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的的N-terminus附近的氨基酸19-42之间
  • cGAS 抗体 (D-9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 cGAS; 不建议用于detection of cGAS of mouse and rat origin; 可能与 an unknown protein of similar size in mouse and rat产生交联反应
  • 抗cGAS抗体（D-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取cGAS (D-9): sc-515777的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 cGAS 抗体 (D-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与cGAS 抗体 (D-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

cGAS抗体（D-9）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测人类来源的cGAS。抗cGAS抗体（D-9）有非结合型和多种 结合形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。环状GMP-AMP合成酶（cGAS）在先天免疫反应中起着至关重要的作用，它充当胞质DNA的传感器，而胞质DNA通常表明病毒感染。环状GMP-AMP合成酶与外来DNA结合后，会催化ATP和GTP合成环状GMP-AMP（cGAMP），后者是一种激活干扰素基因（STING）通路刺激因子的第二信使，可导致产生I型干扰素和其他促炎细胞因子。这一过程对于启动有效的抗病毒反应至关重要，凸显了环状GMP-AMP合成酶在免疫监测和防御病原体方面的重要性。环状GMP-AMP合成酶在包括单核细胞系THP1在内的多种细胞类型中表达，以两种可替代的剪接异构体形式存在，其基因位于人类染色体6q13上。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cGAS 参考文献:

  1. 多种基因产物是 I 型干扰素抗病毒反应的效应物。  |  Schoggins, JW., et al. 2011. Nature. 472: 481-5. PMID: 21478870
  2. 环形 GMP-AMP 合成酶是一种细胞膜 DNA 传感器，可激活 I 型干扰素途径。  |  Sun, L., et al. 2013. Science. 339: 786-91. PMID: 23258413
  3. 先天性免疫 DNA 传感器 cGAS 产生一种非正则环状二核苷酸，可激活人类 STING。  |  Diner, EJ., et al. 2013. Cell Rep. 3: 1355-61. PMID: 23707065
  4. cGAS 细胞质 DNA 感测的结构机制。  |  Civril, F., et al. 2013. Nature. 498: 332-7. PMID: 23722159
  5. 人类 cGAS 的 DNA 感知和免疫激活的结构与功能分析。  |  Kato, K., et al. 2013. PLoS One. 8: e76983. PMID: 24116191
  6. 双链 DNA 诱导的寡聚激活了环 GMP-AMP 合成酶。  |  Li, X., et al. 2013. Immunity. 39: 1019-31. PMID: 24332030
  7. 细胞质 DNA 传感器 cGAS 与 DNA 形成寡聚复合物，并在激活环中发生类似开关的构象变化。  |  Zhang, X., et al. 2014. Cell Rep. 6: 421-30. PMID: 24462292
  8. 结构引导的人类 cGAS 二核苷酸连接特异性重编程。  |  Kranzusch, PJ., et al. 2014. Cell. 158: 1011-1021. PMID: 25131990
  9. TDP-43 通过 mPTP 触发线粒体 DNA 释放，从而激活 ALS 中的 cGAS/STING。  |  Yu, CH., et al. 2020. Cell. 183: 636-649.e18. PMID: 33031745
  10. 作为炎症性疾病治疗靶点的 cGAS-STING 通路。  |  Decout, A., et al. 2021. Nat Rev Immunol. 21: 548-569. PMID: 33833439
  11. 线粒体定位的 cGAS 可抑制铁突变，从而促进癌症进展。  |  Qiu, S., et al. 2023. Cell Res. 33: 299-311. PMID: 36864172
  12. cGAS-STING 驱动与衰老相关的炎症和神经退行性变。  |  Gulen, MF., et al. 2023. Nature. 620: 374-380. PMID: 37532932

订购信息

产品名称产品编号规格价格数量收藏夹

cGAS 抗体 (D-9)

sc-515777
200 µg/ml
$316.00

cGAS (D-9): m-IgG Fc BP-HRP 套装

sc-531510
200 µg Ab; 10 µg BP
$354.00

cGAS (D-9): m-IgGκ BP-HRP 套装

sc-525210
200 µg Ab, 40 µg BP
$354.00

cGAS (D-9): m-IgG2a BP-HRP 套装

sc-547805
200 µg Ab; 10 µg BP
$354.00

cGAS 抗体 (D-9) AC

sc-515777 AC
500 µg/ml, 25% agarose
$416.00

cGAS 抗体 (D-9) HRP

sc-515777 HRP
200 µg/ml
$316.00

cGAS 抗体 (D-9) FITC

sc-515777 FITC
200 µg/ml
$330.00

cGAS 抗体 (D-9) PE

sc-515777 PE
200 µg/ml
$343.00

cGAS 抗体 (D-9) Alexa Fluor® 488

sc-515777 AF488
200 µg/ml
$357.00

cGAS 抗体 (D-9) Alexa Fluor® 546

sc-515777 AF546
200 µg/ml
$357.00

cGAS 抗体 (D-9) Alexa Fluor® 594

sc-515777 AF594
200 µg/ml
$357.00

cGAS 抗体 (D-9) Alexa Fluor® 647

sc-515777 AF647
200 µg/ml
$357.00

cGAS 抗体 (D-9) Alexa Fluor® 680

sc-515777 AF680
200 µg/ml
$357.00

cGAS 抗体 (D-9) Alexa Fluor® 790

sc-515777 AF790
200 µg/ml
$357.00

cGAS Antibody (D-9): sc-515777 does this work for FCM and IHC in mouse tissues and cells? Why do you "GAS Antibody (D-9) is recommended for detection of cGAS of human origin by WB, IP, IF, IHC(P) and ELISA; not recommended for detection of cGAS of mouse"

Asked by: Annamalai
Thank you for your question . Yes we also recommend the antibody for IF,IHC and FCM. The antibody has only been validated for human. There is a 64% homology and 56% homology in rat .
Answered by: BlakeJ
Date published: 2023-10-19

Is this can be used to detect mouse sample?

Asked by: XIBIN
Thank you for your question. As indicated in the product description, cGAS Antibody (D-9) is only recommended for detection of cGAS of human origin by WB, IP, IF, IHC(P) and ELISA. It is not recommended for detection of cGAS of mouse and rat origin.
Answered by: Tech Support Europe
Date published: 2021-03-29

I need samples,but do not know contact who.

Asked by: 年轻的博士
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: Technical Support
Date published: 2018-12-14

CAn I have a free sample for cGAS antibody sc-515777 AF790 and sc-245858. I would like to test them to my cell lysate (THP-1 and HFLS) Thank you

Asked by: SylvieP
We do not provide sample sizes for any of our conjugated primary antibodies. Please contact our Technical Service Department for more information if you would like to receive a free 10 ug sample of this unconjugated monoclonal antibody. Please call us toll-free at (800) 457-3801 option 2, or use the live chat on our website. The other catalog number you provided, sc-245858, is for a discontinued goat polyclonal antibody that is no longer available.
Answered by: Technical Support
Date published: 2018-11-14

I am using cGAS (D-9): sc-515777 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Cweed
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Works for WBIt works well for WB and signal is strong. Dilution 1:1000
Date published: 2024-11-05
Rated 5 out of 5 by from This Ab can recognize mouse cGASI have shown by shRNA knockdown that this antibody (D-9) recognizes mouse cGAS. A band of the same size was strongly detected in human cells (BJ-5ta). Unfortunately, the C-1 antibody did not detect a band of the correct size.
Date published: 2021-09-18
Rated 5 out of 5 by from Excellent antibody for WB and IFI've bought this antibody several times, the quality is consistent and perfect especially for WB and IF staining.
Date published: 2020-12-09
Rated 1 out of 5 by from Not recognizing mouse cGAS!I used cell lysates from primary bone-marrow derived macrophages from C57BL/C6 WT and cGAS KO (ordered from Jackson laboratory cat# 026554) mice for western blot and got a band around 60 kDa in both samples. Clearly, this antibody is not detecting cGAS!
Date published: 2019-05-24
Rated 4 out of 5 by from Good antibodyThis antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 1 out of 5 by from Antibody is not detecting cGAS!The antibody is detecting a protein a correct mass of about 60 kDa. However, the same band is detectable in three different human cGAS-/- clones (BLaER1 cells). The antibody is not specific!
Date published: 2018-08-02
Rated 4 out of 5 by from Works goodI tried a free trial sample of this antibody. I validated this antibody by comparing HeLa with HEK293. HEK293 does not express cGAS so it can be used as a 'KO validation'. Used as 1:1000 in 5% milk TBS-T.
Date published: 2018-05-04
Rated 5 out of 5 by from works well for samples from mouseClear band detected using samples from mouse at a dilution of 1:200 in milk/PBS. We have not tested further dilutions.
Date published: 2018-03-14
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cGAS 抗体 (D-9) is rated 3.9 out of 5 by 14.
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