Date published: 2025-9-6
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Cdk9 Antibody (F-6) - Western Blotting - Image 353143
Cdk9 抗体 (F-6): sc-376646. HeLa (A) 和 KNRK (B) 核提取物以及 Jurkat (C), A-431 (D) 和 HEK293 (E) 全细胞裂解液中 Cdk9 表达的 Western 印迹分析.
Cdk9 Antibody (F-6) - Western Blotting - Image 353143
Cdk9 抗体 (F-6): sc-376646. HeLa (A) 和 KNRK (B) 核提取物以及 Jurkat (C), A-431 (D) 和 HEK293 (E) 全细胞裂解液中 Cdk9 表达的 Western 印迹分析.
Cdk9 Antibody (F-6) - Immunofluorescence - Image 157259
Cdk9 抗体 (F-6): sc-376646. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示细胞核和细胞质定位.
Cdk9 Antibody (F-6) - Western Blotting - Image 157667
Cdk9 抗体 (F-6): sc-376646. HL-60 (A) 和 NIH/3T3 (B) 全细胞裂解液中 Cdk9 表达的 Western 印迹分析.
Cdk9 抗体 (F-6): sc-376646. HeLa (A) 和 KNRK (B) 核提取物以及 Jurkat (C), A-431 (D) 和 HEK293 (E) 全细胞裂解液中 Cdk9 表达的 Western 印迹分析.

Cdk9 抗体 (F-6): sc-376646

4.8(5)
寫評論提問

说明书
  • Cdk9抗体(F-6)是小鼠单克隆IgG1 κ, 在3篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Cdk9的C-terminus的氨基酸343-372之间
  • 推荐用于 mouse, rat 和 human 来源的Cdk9 WB, IP, IF 和 ELISA检测; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 看 Cdk9 (D-7): sc-13130，可查看有标记的 Cdk9 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Cdk9 Antibody (F-6) for WB applications. 的首选辅助检测试剂，这些试剂现与Cdk9 Antibody (F-6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Cdk抗体，包括Cdk1, Cdk11, Cdk2, Cdk3, Cdk4, Cdk5, Cdk6, Cdk7, Cdk8和Cdk9

细胞周期蛋白依赖激酶(Cdks)家族是细胞周期进程的关键调节因子。Cdk家族成员包括Cdc2 p34, Cdk1-9, PISSLRE, KKIALRE, PITSLRE和PCTAIRE 1-3在整个细胞周期中组成性表达。Cdc2 p34活性高峰在有丝分裂期，Cdk2活性在G1晚期或S期早期升高。Cdk4和Cdk6在G1期向S期进展中起关键作用。Cdk3、Cdk5b、PISSLRE、KKIALRE和PCTAIRE 1-3的功能则尚不明确。Cdk9(也被称为PITALRE)已被证明特异性磷酸化视网膜母细胞瘤蛋白。最近克隆的果蝇蛋白P-TEFb被认为是哺乳动物PITALRE的同源物。P-TEFb被证明是HIV Tat转录激活所必需的。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Cdk9 抗体 (F-6)

sc-376646
200 µg/ml
$316.00

Cdk9 (F-6): m-IgG Fc BP-HRP 套装

sc-540622
200 µg Ab; 10 µg BP
$354.00

Cdk9 (F-6): m-IgG1 BP-HRP 套装

sc-542253
200 µg Ab; 20 µg BP
$354.00

Cdk9 (F-6) 中和勝肽

sc-376646 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-376646 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Good for western and co-IPUsed at 1:500 dilution to detect endogenous protein in nuclear extract from HEK293T. 5ug antibody per 500ug nuclear extract IPed CDK9 and cyclin T1 is detected with sc-271348 in CDK9 IP lane.
Date published: 2018-08-02
Rated 5 out of 5 by from Nice WB!This antibody gives clean Western blot results at a dilution of 1:200 in HeLa, Jurkat, KNRK, HEK293 and A-431 lysates.
Date published: 2017-02-02
Rated 5 out of 5 by from Good for co-IPIt works great for co-IP. I haven't tried it for Western blot.
Date published: 2017-01-16
Rated 5 out of 5 by from Works well for IF applicationsWorks well for IF applications, nuclear and cytoplasmic staining in MeOH fixed HeLa cells. -SCBT QC
Date published: 2015-04-10
Rated 5 out of 5 by from Good antibody for westernGood antibody for western, clean background and specific band in HL-60 and NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2014-05-06
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Cdk9 抗体 (F-6) is rated 4.8 out of 5 by 5.
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