Date published: 2025-9-7
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Cdk8 Antibody (D-9) - Western Blotting - Image 354300
Cdk8 抗体 (D-9): sc-13155. Jurkat 核提取物 (A), SW480 (B) 和 MDA-MB-231 (C) 全细胞裂解液中 Cdk8 表达的 Western 印迹分析.
Cdk8 抗体 (D-9): sc-13155. Jurkat 核提取物 (A), SW480 (B) 和 MDA-MB-231 (C) 全细胞裂解液中 Cdk8 表达的 Western 印迹分析.

Cdk8 抗体 (D-9): sc-13155

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说明书
  • Cdk8 抗体 D-9 是小鼠单克隆 IgG1 κ，Cdk8抗体, 在22篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Cdk8的氨基酸326-464
  • Cdk8 抗体 (D-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Cdk8
  • 抗Cdk8抗体（D-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 关于如何获取Cdk8 (D-9): sc-13155的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Cdk8 抗体 (D-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cdk8 抗体 (D-9) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Cdk 抗体 包括 Cdk1, Cdk11, Cdk2, Cdk3, Cdk4, Cdk5, Cdk6, Cdk7, Cdk8和Cdk9

Cdk8抗体（D-9）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学和酶联免疫吸附等方法检测小鼠、大鼠和人源的Cdk8蛋白。Cdk8 (D-9) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。Cdk8在调节细胞周期进展中发挥着关键作用，它是细胞周期蛋白依赖性激酶（Cdks）家族中的一部分，与细胞周期蛋白协同作用，在细胞周期的不同阶段对重要底物进行磷酸化。这种调节对于维持正常的细胞功能以及确保细胞在正确的时间准确分裂至关重要。Cdk8与细胞周期蛋白C和RNA聚合酶II的大亚基形成大型复合物，表明其参与了转录调控。通过调节RNA聚合酶II的活性，Cdk8影响基因表达，而基因表达对于包括生长和分化在内的各种细胞过程至关重要。当与GAL4的DNA结合结构域融合时，Cdk8充当转录激活因子，突出了其在转录控制中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdk8 抗体 (D-9) 参考文献:

  1. 新型 cdc2/CDC28 相关蛋白激酶基因家族的两个成员 PCTAIRE-1 和 PCTAIRE-3。  |  Okuda, T., et al. 1992. Oncogene. 7: 2249-58. PMID: 1437147
  2. 通过抑制 CDK8/19 将抗原特异性效应/记忆 T 细胞转化为表达 Foxp3 的 Treg 细胞。  |  Akamatsu, M., et al. 2019. Sci Immunol. 4: PMID: 31653719
  3. CDK8和CDK19：信号诱导转录的正调控因子和Mediator复合物的负调控因子。  |  Chen, M., et al. 2023. Nucleic Acids Res. 51: 7288-7313. PMID: 37378433
  4. CDK8抑制剂可对抗HIV-1的重新激活，并促进T细胞中的前病毒潜伏。  |  Horvath, RM., et al. 2023. J Virol. 97: e0092323. PMID: 37671866
  5. 鉴定人类细胞周期蛋白依赖性激酶 8，它是细胞周期蛋白 C 的潜在蛋白激酶伙伴。  |  Tassan, JP., et al. 1995. Proc Natl Acad Sci U S A. 92: 8871-5. PMID: 7568034
  6. 细胞周期激酶  |  Pines, J. 1994. Semin Cancer Biol. 5: 305-13. PMID: 7803767
  7. Cyclin C/CDK8 是一种与 RNA 聚合酶 II 相关的新型 CTD 激酶。  |  Rickert, P., et al. 1996. Oncogene. 12: 2631-40. PMID: 8700522
  8. 果蝇 Cdk8，一种与 RNA 聚合酶 II 大亚基相互作用的细胞周期蛋白 C 的激酶伙伴。  |  Leclerc, V., et al. 1996. Mol Biol Cell. 7: 505-13. PMID: 8730095
  9. 细胞周期蛋白, 细胞周期蛋白依赖性激酶和 cdk 抑制剂：对细胞周期控制和癌症的影响。  |  MacLachlan, TK., et al. 1995. Crit Rev Eukaryot Gene Expr. 5: 127-56. PMID: 8845581
  10. 病毒转录激活因子 E1A 和 VP16 与一个大型复合物相互作用，该复合物与 CTD 激酶活性有关，并包含 CDK8。  |  Gold, MO., et al. 1996. Nucleic Acids Res. 24: 3771-7. PMID: 8871557
  11. 细胞周期蛋白依赖性激酶 4 和 6 抑制剂基因家族 p15, p16, p18 和 p19 在白血病和淋巴瘤中的作用。  |  Siebert, R., et al. 1996. Leuk Lymphoma. 23: 505-20. PMID: 9031081
  12. 神经肿瘤学的最新概念：细胞周期--综述。  |  Dirks, PB. and Rutka, JT. 1997. Neurosurgery. 40: 1000-13; discussion 1013-5. PMID: 9149259

订购信息

产品名称产品编号规格价格数量收藏夹

Cdk8 抗体 (D-9)

sc-13155
200 µg/ml
$316.00

Cdk8 (D-9): m-IgG Fc BP-HRP 套装

sc-526545
200 µg Ab; 10 µg BP
$354.00

Cdk8 (D-9): m-IgG1 BP-HRP 套装

sc-531918
200 µg Ab; 20 µg BP
$354.00

Cdk8 抗体 (D-9) AC

sc-13155 AC
500 µg/ml, 25% agarose
$416.00

Cdk8 抗体 (D-9) HRP

sc-13155 HRP
200 µg/ml
$316.00

Cdk8 抗体 (D-9) FITC

sc-13155 FITC
200 µg/ml
$330.00

Cdk8 抗体 (D-9) PE

sc-13155 PE
200 µg/ml
$343.00

Cdk8 抗体 (D-9) Alexa Fluor® 488

sc-13155 AF488
200 µg/ml
$357.00

Cdk8 抗体 (D-9) Alexa Fluor® 546

sc-13155 AF546
200 µg/ml
$357.00

Cdk8 抗体 (D-9) Alexa Fluor® 594

sc-13155 AF594
200 µg/ml
$357.00

Cdk8 抗体 (D-9) Alexa Fluor® 647

sc-13155 AF647
200 µg/ml
$357.00

Cdk8 抗体 (D-9) Alexa Fluor® 680

sc-13155 AF680
200 µg/ml
$357.00

Cdk8 抗体 (D-9) Alexa Fluor® 790

sc-13155 AF790
200 µg/ml
$357.00

Cdk8 抗体 (D-9) TRITC

sc-13155 TRITC
200 µg/ml
$320.00

Basing on the previous question and answer; So, sometimes, 77kDa is possible, right?

Asked by: yu27
Thanks for your follow-up question. Yes, it could be!
Answered by: Tech Service
Date published: 2019-11-19

The question is about molecular weight of CDK8. The first picture is about 77kDa, but the others are 55KDa, which is right? The instruction is 53kDa.

Asked by: yu27
Thank you for your question. The expected molecular weight for CDK8 is around 53-55kDa. Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include phosphorylations, glycosylations or other post-translational modifications.
Answered by: Tech Service
Date published: 2019-11-19

Does this antibody recognise CDK19 as well?

Asked by: edimi
Thank you for your question. This antibody is specific for Cdk8, and does not cross-react with Cdk19.
Answered by: Technical Support
Date published: 2019-08-17

Can Cdk8 (D-9): sc-13155 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Cdk8 (D-9): sc-13155 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Didnot work for WBDid not work for western blot in MCF7 breast cancer cell line
Date published: 2017-11-21
Rated 5 out of 5 by from good qualityNever meet difficulties use this antibody, very easy operated at 1:500 dilution and got strong band at 53kda.I suggest the loading amount reference to the papers about the expression of the target protein.For high expresion sample loading 30ug is enough.
Date published: 2017-09-10
Rated 5 out of 5 by from VERY GOOD AntiibodyI got this antibody for my research and it gives very nice band with no background. We loaded 25 ug of protein and the bands were clear and crisp.
Date published: 2017-09-04
Rated 5 out of 5 by from western blotI used this antibody for western blot and got great results.I blotted cell lysates from primary hippocampal neurons. The signal was not very powerful and no background.
Date published: 2017-08-26
Rated 4 out of 5 by from goodIt is strong with 1:2000 dilution but has several weak non-specific bands far away in many different types of colon cancer cells.
Date published: 2017-05-12
Rated 5 out of 5 by from good qualityThis antibody has good quality and specific . I found clear bands, strong signals.
Date published: 2017-05-09
Rated 5 out of 5 by from Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-30
Rated 5 out of 5 by from Good Cdk8 control antibody for siRNA KO experimentGood Cdk8 control antibody for siRNA KO experiment -SCBT QC
Date published: 2015-04-13
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Cdk8 抗体 (D-9) is rated 4.5 out of 5 by 11.
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