CD3-zeta抗体 (E-3) 推荐用于 mouse, rat 和 human 来源的CD3-ζ WB, IP, IF 和 ELISA检测

CD3-ζ 抗体 (E-3): sc-166435

CD3-ζ抗体 (E-3) is rated 4.4 out of 5 by 5.
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说明书

    • CD3-ζ抗体(E-3)是小鼠单克隆IgG3 κ, 在3篇文献中引用,规格为200 µg/ml
    • 特异性抗原位于human物种的CD3-ζ的C-terminus的氨基酸137-163之间
    • 推荐用于 mouse, rat 和 human 来源的CD3-ζ WB, IP, IF 和 ELISA检测; 也和以下物种反应,包括: 和 canine, bovine and porcine
    • 看 CD3-ζ (6B10.2): sc-1239,可查看有标记的 CD3-ζ 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
    • 关于如何获取CD3-ζ (E-3): sc-166435免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for CD3-ζ Antibody (E-3) for WB applications. This reagent is now offered in a bundle with CD3-ζ Antibody (E-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Can you recommend a negative control to use with CD3-ζ (E-3): sc-166435 monoclonal antibody?

Asked by: jenni
We recommend using the blocking peptide sc-166435 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 15
Date published: 2017-01-12

Is CD3-ζ (E-3): sc-166435 monoclonal antibody available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using CD3-ζ (6B10.2): sc-1239, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/cd3-zeta-antibody-6b10-2)
Answered by: Technical Support 15
Date published: 2017-01-12
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Rated 2 out of 5 by from Not ideal for immuno-staining HEK cells I tried staining HEK293 cells that were transiently expressing a construct with the CD3 peptide and found that the signal was not very good compared to a parallel immuno-staining with another primary antibody
Date published: 2019-06-05
Rated 5 out of 5 by from good effect Our aim is to use T cell antigen receptor recognition of foreign antigens, and this recognition events into intracellular signals, causing cells from dormant to active state changes. This is a very good effect
Date published: 2017-02-09
Rated 5 out of 5 by from Great for Western blot Good CD-3 zeta antibody; gives great bands in Western blot. Cross-reactive with multiple species--mouse, rat, human, canine, bovine, porcine. Blocking peptide good as a negative control to check specificity.
Date published: 2017-01-27
Rated 5 out of 5 by from Good monoclonal antibody Good monoclonal antibody, favorable data produced in WB on Jurkat whole cell lysate. -SCBT QC
Date published: 2015-01-27
Rated 5 out of 5 by from Superb antibody for Western Blot Superb antibody for Western Blot. Good specificity against human CD3- transfected 293T whole cell lysate. -SCBT QC
Date published: 2013-07-04
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