CD3-zeta抗体 (D-4) 推荐用于 mouse, rat 和 human 来源的CD3-ζ WB, IP, IF, IHC(P) 和 ELISA检测

CD3-ζ 抗体 (D-4): sc-374281

CD3-ζ抗体 (D-4) is rated 4.7 out of 5 by 3.
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说明书

    • CD3-ζ抗体(D-4)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
    • 免疫human物种的代表全长CD3-ζ的氨基酸1-163
    • 抗-CD3-zeta 抗体 (D-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 CD3-ζ
    • 抗-CD3-zeta 抗体 (D-4) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取CD3-ζ (D-4): sc-374281免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for CD3-ζ Antibody (D-4) for WB and IHC(P) applications. These reagents are now offered in bundles with CD3-ζ Antibody (D-4) (see ordering information below).

Can CD3-ζ (D-4): sc-374281 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp
Thank you for your question. Yes, CD3-ζ (D-4): sc-374281 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Positive signal in human CD3 Positive signal in human CD3- transfected 293T whole cell lysates for WB. -SCBT QC
Date published: 2015-03-22
Rated 5 out of 5 by from Beautiful immunoperoxidase membrane and cytoplasmic Beautiful immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-03-04
Rated 5 out of 5 by from Works great for Jurkat whole cell lysate Works great for Jurkat whole cell lysate. -SCBT QC
Date published: 2015-02-12
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