Date published: 2025-9-6
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CD14 Antibody (H-4) - Western Blotting - Image 364820
CD14 抗体 (H-4): sc-515785. BJAB (A), AMJ2-C8 (B), NRK (C) 和 H19-7/IGF-IR (D) 全细胞裂解液中 CD14 表达的 Western 印迹分析. 注意 A 道中与人 CD14 缺乏反应, B 道中与小鼠 CD14 缺乏反应.
CD14 Antibody (H-4) - Western Blotting - Image 364820
CD14 抗体 (H-4): sc-515785. BJAB (A), AMJ2-C8 (B), NRK (C) 和 H19-7/IGF-IR (D) 全细胞裂解液中 CD14 表达的 Western 印迹分析. 注意 A 道中与人 CD14 缺乏反应, B 道中与小鼠 CD14 缺乏反应.
CD14 Antibody (H-4) - Western Blotting - Image 379034
CD14 抗体 (H-4): sc-515785. 对 AT3B-1 全细胞裂解液中 CD14 表达的 Western 印迹分析.
CD14 抗体 (H-4): sc-515785. BJAB (A), AMJ2-C8 (B), NRK (C) 和 H19-7/IGF-IR (D) 全细胞裂解液中 CD14 表达的 Western 印迹分析. 注意 A 道中与人 CD14 缺乏反应, B 道中与小鼠 CD14 缺乏反应.

CD14 抗体 (H-4): sc-515785

4.3(3)
寫評論提問

说明书
  • CD14抗体(H-4)是小鼠单克隆IgG2a κ, 在7篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于rat物种的的N-terminus附近的氨基酸54-73之间
  • 抗-CD14 抗体 (H-4) 推荐用于 WB, IP, IF 和 ELISA，检测mouse 和rat 来源的 CD14
  • 抗CD14抗体（H-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD14 (H-4): sc-515785的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是CD14 Antibody (H-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CD14 Antibody (H-4) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD14抗体（H-4）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）特异性检测小鼠和大鼠来源的CD14。CD14，也称为单核细胞分化抗原CD14或髓细胞特异性亮氨酸富集糖蛋白，是一种在单核细胞、巨噬细胞和中性粒细胞表面表达的糖磷脂酰肌醇（GPI）锚定蛋白。CD14在先天免疫系统中起着关键作用，它充当细菌脂多糖（LPS）的辅助受体，而细菌脂多糖是革兰氏阴性细菌外膜的组成部分。结合LPS后，CD14有助于激活Toll样受体4（TLR4）信号通路，从而产生促炎性细胞因子和介质，这些物质对于启动免疫反应以抵抗感染至关重要。CD14单克隆抗体（H-4）识别CD14 N端附近54-73位氨基酸之间的特定表位。抗CD14抗体（H-4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，这使得CD14（H-4）抗体可用于各种免疫学检测和研究应用，涉及CD14相关的信号通路。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

CD14 抗体 (H-4)

sc-515785
200 µg/ml
$316.00

CD14 (H-4): m-IgG Fc BP-HRP 套装

sc-531513
200 µg Ab; 10 µg BP
$354.00

CD14 (H-4): m-IgGκ BP-HRP 套装

sc-525215
200 µg Ab, 40 µg BP
$354.00

CD14 (H-4): m-IgG2a BP-HRP 套装

sc-547806
200 µg Ab; 10 µg BP
$354.00

CD14 抗体 (H-4) AC

sc-515785 AC
500 µg/ml, 25% agarose
$416.00

CD14 抗体 (H-4) HRP

sc-515785 HRP
200 µg/ml
$316.00

CD14 抗体 (H-4) FITC

sc-515785 FITC
200 µg/ml
$330.00

CD14 抗体 (H-4) PE

sc-515785 PE
200 µg/ml
$343.00

CD14 抗体 (H-4) Alexa Fluor® 488

sc-515785 AF488
200 µg/ml
$357.00

CD14 抗体 (H-4) Alexa Fluor® 546

sc-515785 AF546
200 µg/ml
$357.00

CD14 抗体 (H-4) Alexa Fluor® 594

sc-515785 AF594
200 µg/ml
$357.00

CD14 抗体 (H-4) Alexa Fluor® 647

sc-515785 AF647
200 µg/ml
$357.00

CD14 抗体 (H-4) Alexa Fluor® 680

sc-515785 AF680
200 µg/ml
$357.00

CD14 抗体 (H-4) Alexa Fluor® 790

sc-515785 AF790
200 µg/ml
$357.00

hi there, is this antibody suitable for neutralization of CD14 in murine cells? thanks

Asked by: Tewfikhamidi
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2023-06-14

Is H-4 a function blocking antibody for rat CD14 and is it formulated in amounts and in buffers appropriate for in vivo studies (i.e. no azide, no carrier protein)?

Asked by: DTCJ
Thank you for your question. We have not tested this antibody for any functional blocking activity, nor are we aware of any publications citing use of the antibody for any blocking function. If you wish to test the antibody yourself for this application, please contact our Special Orders Department for information about ordering it without sodium azide or carrier protein. Please call us toll-free at (800) 457-3801 option 2, or email specialorders@scbt.com.
Answered by: Technical Support
Date published: 2019-07-16

How much sample do you recommend loading on the gel for Western blot detection using CD14 (H-4): sc-515785?

Asked by: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from works very wellThis antibody works very well by WB.We tried different conditons and optimized the loading amount and tried different dilution 1:100 to 1:800 all tried.The optimiezd dilution 1:300 is the best.
Date published: 2018-05-26
Rated 3 out of 5 by from Dr.This antibody has two more non-special bands in my blot, when I detected it in the MLE-12 cells
Date published: 2017-10-02
Rated 5 out of 5 by from Identifying and removing macrophageThis antibody is really good for identifying and removing macrophage as contaminating cells in isolates in conjugation with dynabeads magnetic beads. Therefore this is a must have item, will definitely be stocking up!
Date published: 2017-09-14
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CD14 抗体 (H-4) is rated 4.3 out of 5 by 3.
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