Date published: 2025-9-7
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CD14 Antibody (UCH-M1) - Western Blotting - Image 4742
CD14 抗体 (UCH-M1): sc-1182. BJAB 全细胞裂解液中 CD14 表达的 Western 印迹分析.
CD14 抗体 (UCH-M1): sc-1182. BJAB 全细胞裂解液中 CD14 表达的 Western 印迹分析.

CD14 抗体 (UCH-M1): sc-1182

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说明书
  • CD14抗体(UCH-M1)是小鼠单克隆IgG2a κ, 在46篇文献中引用，规格为200 µg/ml
  • 针对human胸腺细胞/塞扎里T细胞
  • CD14 抗体 (UCH-M1) 推荐用于WB, IP, IF, IHC(P) 和 FCM，检测human 来源的 CD14 monocyte specific cell surface protein
  • 抗CD14抗体（UCH-M1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD14 (UCH-M1): sc-1182的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 CD14 抗体 (UCH-M1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与CD14 抗体 (UCH-M1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD14抗体（UCH-M1）是一种IgG2a κ小鼠单克隆CD14抗体，可通过WB、IP、IF、IHC（P）和FCM检测人类来源的CD14单核细胞特异性细胞表面蛋白。CD14抗体（UCH-M1）既可以是非偶联的抗CD14抗体形式，也可以是多种偶联形式的抗CD14抗体，包括琼脂、HRP、PE、FITC和多种Alexa Fluor®偶联物。脂多糖（LPS）引发活化巨噬细胞和单核细胞分泌介质和细胞因子。CD14是一种糖基磷脂酰肌醇（GPI）抗体，可在单核细胞和多形核白细胞表面发现。CD14充当LPS受体，导致各种蛋白质的分泌。LPS通过CD14受体激活单核细胞的一个重要组成部分是"适配分子"脂多糖结合蛋白（LBP）。CD14有两种形式，一种是膜结合形式（mCD14），另一种是可溶性形式（sCD14）。mCD14仅对LPS做出反应，促进蛋白质的分泌，而不表达mCD14的细胞对LPS无反应。缺乏mCD14的细胞在sCD14存在下对LPS / LBP做出反应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD14 抗体 (UCH-M1) 参考文献:

  1. 脂多糖（LPS）结合蛋白（LBP）和 LPS/LBP 复合物受体 CD14 的功能：简评。  |  Schumann, RR. 1992. Res Immunol. 143: 11-5. PMID: 1373512
  2. 单核细胞抗原 CD14 是一种磷脂锚定膜蛋白。  |  Simmons, DL., et al. 1989. Blood. 73: 284-9. PMID: 2462937
  3. 可溶性脂多糖受体（CD14）通过两种不同的机制从人类单核细胞和 CD14 转染细胞中释放出来。  |  Bufler, P., et al. 1995. Eur J Immunol. 25: 604-10. PMID: 7533093
  4. 脂多糖结合蛋白和 CD14 可调节受脂多糖刺激的人单核细胞, 间质细胞和内皮细胞合成血小板活化因子。  |  Camussi, G., et al. 1995. J Immunol. 155: 316-24. PMID: 7541418
  5. CD14和其他脂多糖识别分子：综述。  |  Kielian, TL. and Blecha, F. 1995. Immunopharmacology. 29: 187-205. PMID: 7542643
  6. 成人呼吸窘迫综合征中中性粒细胞对内毒素的反应：CD14 的作用。  |  Parsons, PE., et al. 1995. Am J Respir Cell Mol Biol. 13: 152-60. PMID: 7542895
  7. 内毒素受体（CD14）与 CD16（Fc γ RIII）一起存在于中性粒细胞的细胞内，细胞内含有碱性磷酸酶。  |  Detmers, PA., et al. 1995. J Immunol. 155: 2085-95. PMID: 7543538

订购信息

产品名称产品编号规格价格数量收藏夹

CD14 抗体 (UCH-M1)

sc-1182
200 µg/ml
$316.00

CD14 (UCH-M1): m-IgG Fc BP-HRP 套装

sc-525425
200 µg Ab; 10 µg BP
$354.00

CD14 (UCH-M1): m-IgG2a BP-HRP 套装

sc-546248
200 µg Ab; 10 µg BP
$354.00

CD14 抗体 (UCH-M1) AC

sc-1182 AC
500 µg/ml, 25% agarose
$416.00

CD14 抗体 (UCH-M1) HRP

sc-1182 HRP
200 µg/ml
$316.00

CD14 抗体 (UCH-M1) FITC

sc-1182 FITC
200 µg/ml
$330.00

CD14 抗体 (UCH-M1) PE

sc-1182 PE
200 µg/ml
$343.00

CD14 抗体 (UCH-M1) Alexa Fluor® 488

sc-1182 AF488
200 µg/ml
$357.00

CD14 抗体 (UCH-M1) Alexa Fluor® 546

sc-1182 AF546
200 µg/ml
$357.00

CD14 抗体 (UCH-M1) Alexa Fluor® 594

sc-1182 AF594
200 µg/ml
$357.00

CD14 抗体 (UCH-M1) Alexa Fluor® 647

sc-1182 AF647
200 µg/ml
$357.00

CD14 抗体 (UCH-M1) Alexa Fluor® 680

sc-1182 AF680
200 µg/ml
$357.00

CD14 抗体 (UCH-M1) Alexa Fluor® 790

sc-1182 AF790
200 µg/ml
$357.00

What is the CD14 concentration used for flow cytometry?

Asked by: Bravo_biologist
Thank you for your question. You can find our recommended protocol for flow cytometry under this link: https://www.scbt.com/resources/protocols/flow-cytometry. We recommend using 1 µg of fluorochrome-conjugated antibodies per 1 million cells in 100 µl buffer per tube.
Answered by: Tech Support Europe
Date published: 2023-03-21

What negative control should be used when using the conjugated version of CD14 (UCH-M1): sc-1182 monoclonal antibody in flow cytometry?

Asked by: jenniferc
Thank you for your inquiry. We recommend using the isotype specific normal mouse IgG conjugated to the same fluorophore. For example, if you are using sc-1182 PE, then you would want to use normal mouse IgG2a PE, sc-2867.
Answered by: Technical Support 9
Date published: 2017-01-11

What secondary is best to use with CD14 (UCH-M1): sc-1182 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with CD14 (UCH-M1): sc-1182, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-11
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Rated 4 out of 5 by from good antibody for IFI used this antibody in IF in frozen human colon tissue sections and it works very well.
Date published: 2018-06-20
Rated 5 out of 5 by from OutstandingGreat western blot data; strong signal, lowbackground; also superb FCM and IHC results
Date published: 2017-01-28
Rated 5 out of 5 by from Great, versatile antibodyThe CD14 Antibody (UCH-M1): sc-1182 works well for a variety of applications including WB, IF, IHC(P) and FCM. This antibody has also been cited over 30 times for further verification.
Date published: 2017-01-12
Rated 5 out of 5 by from works great for IFUsed it at 1:50-1:100 dilution on PBMCs for 1hr at RT. Worked great, positive cells very bright with no non-specific binding.
Date published: 2016-11-07
Rated 1 out of 5 by from tested in ASTMAtested in ASTMA-23, and Lymph TMA tissue for IHC. Negative @1:25; Not a Good Antibody for IHC
Date published: 2016-06-13
Rated 4 out of 5 by from Various publishable data in FCM using humanVarious publishable data in FCM using human peripheal blood, THP-1 and PBMC cell lines. -SCBT Publication Review
Date published: 2015-05-19
Rated 4 out of 5 by from Various publishable data in WB using humanVarious publishable data in WB using human LPS and PGN stimulated uroepithelial cell lines T24, 5637, UM-UC-3, and HT1197, transf. K562 and Human oviduct cell lines. -SCBT Publication Review
Date published: 2015-03-01
Rated 4 out of 5 by from Great immunoperoxidase membrane stainingGreat immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded normal human lymph node. -SCBT QC
Date published: 2015-02-24
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CD14 抗体 (UCH-M1) is rated 4.0 out of 5 by 12.
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