Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CBX7 CRISPR/Cas9 KO Plasmid (m): sc-424634
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CBX7 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CBX7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CBX7 Antibody (G-3): sc-376274
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CBX7 CRISPR/Cas9 KO Plasmid (m) | sc-424634 | 20 µg | $397.00 |
Overview
Cbx7 encodes chromobox protein homolog 7 (CBX7), a chromodomain-containing reader of H3K27me3 that functions as a core component of Polycomb repressive complex 1 (PRC1) to maintain stable transcriptional silencing. In mouse cells, CBX7 contributes to epigenetic control of lineage commitment, cell-cycle regulation, and senescence by promoting chromatin compaction and repression of developmental and tumor suppressor loci. CBX7-dependent Polycomb activity intersects with differentiation programs and stem cell self-renewal networks, shaping long-term gene expression states. Dysregulation of Polycomb-mediated repression, including altered CBX7 function, is widely studied in oncogenesis, aging-associated phenotypes, and developmental disorders as a mechanism of aberrant chromatin regulation.
CBX7 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cbx7 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cbx7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cbx7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CBX7 protein expression.
This CRISPR knockout system enables efficient generation of Cbx7-deficient cell models for investigation of CBX7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cbx7 exon(s) critical for CBX7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cbx7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CBX7 CRISPR/Cas9 KO Plasmid (m) and CBX7 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cbx7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CBX7 HDR Plasmid (m) and CBX7 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cbx7 homology arms to support homology-directed repair at defined Cbx7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.