Date published: 2025-9-6

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CBX7 抗体 (G-3): sc-376274

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说明书
  • CBX7 抗体 G-3 是小鼠单克隆 IgG1 κ,CBX7抗体, 在6篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列74-158位于mouse物种的CBX7的C-terminus
  • CBX7 抗体 (G-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 CBX7
  • 抗CBX7抗体(G-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-376274 X, 200 µg/0.1 ml)
  • 关于如何获取CBX7 (G-3): sc-376274免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是CBX7 Antibody (G-3) for WB applications. 的首选辅助检测试剂,这些试剂现与CBX7 Antibody (G-3) 打包提供(请参阅下面的订购信息)。

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関連項目

CBX7抗体(G-3)是一种IgG1 κ小鼠单克隆CBX7抗体(也称为CBX7抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的CBX7蛋白。CBX7抗体(G-3)既有非偶联型抗CBX7抗体形式,也有多种偶联型抗CBX7抗体形式,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。CBX7(Chromobox蛋白同源物7)是一种由251个氨基酸组成的核蛋白,包含一个N端chromo结构域和一个C端Pc盒。CBX7在肾、脑、心脏和骨骼肌中高表达,在外周血白细胞中表达较弱,作为染色质相关多梳复合体(PcG)的组分发挥作用,参与维持目标基因的转录抑制状态。此外,CBX7修饰染色质,并通过抑制p14 ARF表达来延长上皮细胞的寿命,同时抑制端粒酶活性。由于其抑制细胞周期相关蛋白转录的能力,CBX7被认为在肿瘤发生中发挥作用,特别是在滤泡性淋巴瘤和甲状腺癌的发展中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CBX7 抗体 (G-3) 参考文献:

  1. 多聚酶CBX7在细胞寿命中具有统一的作用。  |  Gil, J., et al. 2004. Nat Cell Biol. 6: 67-72. PMID: 14647293
  2. 多聚胞群蛋白在干细胞自我更新和癌症中的作用  |  Gil, J., et al. 2005. DNA Cell Biol. 24: 117-25. PMID: 15699631
  3. 小儿脑外胶质瘤的芯片分析确定了一个由 112 个候选基因组成的基因簇,其中包括 22q12.1-q13.3 处的四个转录本。  |  Suarez-Merino, B., et al. 2005. Neuro Oncol. 7: 20-31. PMID: 15701279
  4. CBX7 通过抑制 Ink4a/Arf 基因座来控制正常和肿瘤衍生物前列腺细胞的生长。  |  Bernard, D., et al. 2005. Oncogene. 24: 5543-51. PMID: 15897876
  5. 小鼠多聚核蛋白与甲基化组蛋白 H3 和 RNA 的结合方式不同,并且富集在变异异染色质中。  |  Bernstein, E., et al. 2006. Mol Cell Biol. 26: 2560-9. PMID: 16537902
  6. 染色体盒蛋白 CBX7 在淋巴瘤发生中的作用  |  Scott, CL., et al. 2007. Proc Natl Acad Sci U S A. 104: 5389-94. PMID: 17374722
  7. CBX7基因表达的缺失与甲状腺癌的高度恶性表型相关。  |  Pallante, P., et al. 2008. Cancer Res. 68: 6770-8. PMID: 18701502
  8. Polycomb蛋白家族成员CBX7在癌症进展中起着关键作用。  |  Pallante, P., et al. 2015. Am J Cancer Res. 5: 1594-601. PMID: 26175930
  9. CBX7通过调节AKR1B10-ERK信号传导抑制膀胱癌进展。  |  Huang, Z., et al. 2021. Cell Death Dis. 12: 537. PMID: 34035231
  10. CBX7在癌症进展中具有双重作用,取决于其功能和分子相互作用。  |  Li, J., et al. 2021. Front Genet. 12: 740794. PMID: 34659360
  11. 多孔类群蛋白 CBX7 通过调节 TARDBP/RBM38 轴抑制心肌细胞增殖  |  Cho, KW., et al. 2023. Circulation. 147: 1823-1842. PMID: 37158107
  12. CBX7沉默通过与BMI1相互作用并激活Nrf2/ARE信号通路促进肝脏再生。  |  Dou, Z., et al. 2024. Sci Rep. 14: 11008. PMID: 38744845

订购信息

产品名称产品编号规格价格数量收藏夹

CBX7 抗体 (G-3)

sc-376274
200 µg/ml
$316.00

CBX7 (G-3): m-IgG Fc BP-HRP 套装

sc-527518
200 µg Ab; 10 µg BP
$354.00

CBX7 (G-3): m-IgG1 BP-HRP 套装

sc-532891
200 µg Ab; 20 µg BP
$354.00

CBX7 抗体 (G-3) X

sc-376274 X
200 µg/0.1 ml
$316.00

CBX7 抗体 (G-3) AC

sc-376274 AC
500 µg/ml, 25% agarose
$416.00

CBX7 抗体 (G-3) HRP

sc-376274 HRP
200 µg/ml
$316.00

CBX7 抗体 (G-3) FITC

sc-376274 FITC
200 µg/ml
$330.00

CBX7 抗体 (G-3) PE

sc-376274 PE
200 µg/ml
$343.00

CBX7 抗体 (G-3) Alexa Fluor® 488

sc-376274 AF488
200 µg/ml
$357.00

CBX7 抗体 (G-3) Alexa Fluor® 546

sc-376274 AF546
200 µg/ml
$357.00

CBX7 抗体 (G-3) Alexa Fluor® 594

sc-376274 AF594
200 µg/ml
$357.00

CBX7 抗体 (G-3) Alexa Fluor® 647

sc-376274 AF647
200 µg/ml
$357.00

CBX7 抗体 (G-3) Alexa Fluor® 680

sc-376274 AF680
200 µg/ml
$357.00

CBX7 抗体 (G-3) Alexa Fluor® 790

sc-376274 AF790
200 µg/ml
$357.00

I have been trying to use this antibody with the fluorphore 488 attached to it for IF so far I have been getting all background can I get some help in trouble shooting it

Asked by: CTM Jefferson
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody. Our recommended protocol for direct immunofluorescence staining is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2019-01-24

Are there any issues with using sc-376274: CBX7 (G-3) mouse monoclonal primary antibody on mouse samples?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-376274 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 1 out of 5 by from Does not work at allI purchased this antibody and tested with lysates of cells where CBX7 was overexpressed via adenoviral transduction. This antibody detected certain proteins but those were not CBX7 protein. Rather I tested the same cell lysates with an antibody from Abcam and it did work great.
Date published: 2020-01-02
Rated 5 out of 5 by from Great Western blot data of CBX7 expression in nonGreat Western blot data of CBX7 expression in non-transfected and mouse CBX7 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-04-20
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CBX7 抗体 (G-3) is rated 3.0 out of 5 by 2.
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