caspase-2 Antibody (1C10): sc-53928

caspase-2 Antibody (1C10) is a mouse monoclonal IgG2b antibody that detects caspase-2 in human samples through applications such as western blotting (WB) and immunoprecipitation (IP). Caspase-2 is an aspartate-specific cysteine protease that plays a critical role in the apoptotic process, acting as a key regulator of programmed cell death. The unique structure of caspase-2, characterized by a long prodomain and DEXD substrate specificity, allows caspase-2 to function both as an upstream initiator and a downstream executor of apoptosis, making caspase-2 essential for maintaining cellular homeostasis. Notably, caspase-2 is predominantly expressed in the brain during development, with lower levels found in adult tissues, and localizes to various cellular compartments, including the mitochondria, Golgi apparatus, cytoplasm, and nucleus. The presence of two isoforms, caspase-2L and caspase-2S, produced through alternative splicing, further highlights this complexity; while caspase-2L promotes apoptosis, caspase-2S serves as a negative regulator, illustrating the delicate balance of apoptotic signaling pathways. Following apoptotic stimuli, the caspase-2L precursor is cleaved to generate active fragments, including p30, p18, and p12, which are crucial for executing the apoptotic program. The ability of caspase-2 to interact with various cellular proteins underscores caspase-2′s importance in apoptosis and cellular signaling, making anti-caspase-2 antibody (1C10) a valuable tool for researchers studying these critical processes.

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caspase-2 Antibody (1C10) References:

1. ER Stress Drives Lipogenesis and Steatohepatitis via Caspase-2 Activation of S1P.  |  Kim, JY., et al. 2018. Cell. 175: 133-145.e15. PMID: 30220454
2. Caspase-2 Substrates: To Apoptosis, Cell Cycle Control, and Beyond.  |  Brown-Suedel, AN. and Bouchier-Hayes, L. 2020. Front Cell Dev Biol. 8: 610022. PMID: 33425918
3. Caspase-2 as a master regulator of genomic stability.  |  Kopeina, GS. and Zhivotovsky, B. 2021. Trends Cell Biol. 31: 712-720. PMID: 33752921
4. Caspase-2 mRNA levels are not elevated in mild cognitive impairment, Alzheimer's disease, Huntington's disease, or Lewy Body dementia.  |  Hlynialuk, C., et al. 2022. PLoS One. 17: e0274784. PMID: 36129947
5. Unveiling caspase-2 regulation by non-coding RNAs.  |  Zhao, Y., et al. 2022. Cell Death Dis. 13: 834. PMID: 36171196
6. ER stress induces caspase-2-tBID-GSDME-dependent cell death in neurons lytically infected with herpes simplex virus type 2.  |  Ren, F., et al. 2023. EMBO J. 42: e113118. PMID: 37646198
7. Caspase-2 protects against ferroptotic cell death.  |  Dawar, S., et al. 2024. Cell Death Dis. 15: 182. PMID: 38429264
8. Caspase-2 is essential for proliferation and self-renewal of nucleophosmin-mutated acute myeloid leukemia.  |  Sakthivel, D., et al. 2024. Sci Adv. 10: eadj3145. PMID: 39093977
9. Caspase-2 kills cells with extra centrosomes.  |  Rizzotto, D., et al. 2024. Sci Adv. 10: eado6607. PMID: 39475598
10. Caspase-2 is a condensate-mediated deubiquitinase in protein quality control.  |  Ge, Y., et al. 2024. Nat Cell Biol. 26: 1943-1957. PMID: 39482354

The preferred caspase-2_L antibody is caspase-2_L Antibody (F-7): sc-5292.