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Caper 抗体 (G-10): sc-376531

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说明书
  • Caper 抗体 G-10 是小鼠单克隆 IgG1 κ,Caper抗体, 在7篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列428-530位于human物种的Caper的C-terminus
  • Caper 抗体 (G-10) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Caper; 也和以下物种反应,包括: 和 equine, canine, bovine and porcine
  • 抗Caper抗体(G-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Caper (G-10): sc-376531免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Caper Antibody (G-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Caper Antibody (G-10) 打包提供(请参阅下面的订购信息)。

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関連項目

Caper抗体(G-10)是一种IgG1κ小鼠单克隆Caper抗体(也称为Caper抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Caper蛋白。 Caper抗体(G-10)既可以作为非偶联的抗Caper抗体形式,也可以作为多种偶联形式的抗Caper抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。 Caper,也称为剪接因子HCC1或肝细胞癌蛋白1和RNA结合区包含蛋白2(RNPC2),作为类固醇核受体c-Jun、ERalpha和ER-beta的转录辅激活因子。 Caper是一种核蛋白,在核斑点中浓度最高,在mRNA前体剪接过程中发挥作用。 Caper的两种同工型HCC1.3和HCC1.4与mRNA前体剪接因子SC35和富含尿苷的小核RNA共定位。 Caper是一种广泛表达的蛋白质,在骨骼肌、肺、脑和胰腺中检测到的水平最高。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Caper 抗体 (G-10) 参考文献:

  1. 人类染色体17q11.2上的趋化因子基因簇的组织,包含CC趋化因子MPIF-1、HCC-2、HCC-1、LEC和RANTES的基因。  |  Nomiyama, H., et al. 1999. J Interferon Cytokine Res. 19: 227-34. PMID: 10213461
  2. 人类趋化因子HCC-1的翻译后加工形式。  |  Richter, R., et al. 2000. Biochemistry. 39: 10799-805. PMID: 10978165
  3. 活化蛋白-1 和雌激素受体的新型辅激活剂 CAPER 的分子克隆和特征描述。  |  Jung, DJ., et al. 2002. J Biol Chem. 277: 1229-34. PMID: 11704680
  4. Hcc-1 是核基质的一种新成分,具有生长抑制功能。  |  Leaw, CL., et al. 2004. Cell Mol Life Sci. 61: 2264-73. PMID: 15338056
  5. U2AF65 相关蛋白 CAPERalpha 和 CAPERbeta 对类固醇激素受体的共激活作用和替代 RNA 剪接。  |  Dowhan, DH., et al. 2005. Mol Cell. 17: 429-39. PMID: 15694343
  6. Pre-mRNA 剪接的第二步需要一种新型 SR 相关蛋白。  |  Cazalla, D., et al. 2005. Mol Cell Biol. 25: 2969-80. PMID: 15798186
  7. HCC1是一种聚半乳糖醛酸酶,通过乙烯合成途径调节叶绿素降解。  |  Liao, Y., et al. 2023. Rice (N Y). 16: 57. PMID: 38071259
  8. HCC-1通过诱导内皮细胞和巨噬细胞热解作用加速动脉粥样硬化,并作为早期诊断生物标志物。  |  Bu, F., et al. 2024. Arterioscler Thromb Vasc Biol. 44: 2088-2107. PMID: 39087347
  9. 利用肝细胞癌抗体鉴定出具有剪接因子图案的新型核自身抗原。  |  Imai, H., et al. 1993. J Clin Invest. 92: 2419-26. PMID: 8227358
  10. HCC-1是一种来自人血浆的新型趋化因子。  |  Schulz-Knappe, P., et al. 1996. J Exp Med. 183: 295-9. PMID: 8551235
  11. C-C趋化因子受体1(CCR1)被鉴定为单核细胞血滤C-C趋化因子(HCC)-1受体。  |  Tsou, CL., et al. 1998. J Exp Med. 188: 603-8. PMID: 9687537
  12. 绘制在胎儿骨骼肌中表达的猪 FBN2, YWHAQ, CNN3, DCN, POSTN, SPARC, RBM39 和 GNAS 基因图。  |  Stratil, A., et al. 2008. Anim Genet. 39: 204-5. PMID: 18162102

订购信息

产品名称产品编号规格价格数量收藏夹

Caper 抗体 (G-10)

sc-376531
200 µg/ml
$316.00

Caper (G-10): m-IgGκ BP-HRP 套装

sc-522994
200 µg Ab, 40 µg BP
$354.00

Caper (G-10): m-IgG1 BP-HRP 套装

sc-533541
200 µg Ab; 20 µg BP
$354.00

Caper 抗体 (G-10) AC

sc-376531 AC
500 µg/ml, 25% agarose
$416.00

Caper 抗体 (G-10) HRP

sc-376531 HRP
200 µg/ml
$316.00

Caper 抗体 (G-10) FITC

sc-376531 FITC
200 µg/ml
$330.00

Caper 抗体 (G-10) PE

sc-376531 PE
200 µg/ml
$343.00

Caper 抗体 (G-10) Alexa Fluor® 488

sc-376531 AF488
200 µg/ml
$357.00

Caper 抗体 (G-10) Alexa Fluor® 546

sc-376531 AF546
200 µg/ml
$357.00

Caper 抗体 (G-10) Alexa Fluor® 594

sc-376531 AF594
200 µg/ml
$357.00

Caper 抗体 (G-10) Alexa Fluor® 647

sc-376531 AF647
200 µg/ml
$357.00

Caper 抗体 (G-10) Alexa Fluor® 680

sc-376531 AF680
200 µg/ml
$357.00

Caper 抗体 (G-10) Alexa Fluor® 790

sc-376531 AF790
200 µg/ml
$357.00

What is the recommended fixation method for immunofluorescence staining with Caper (G-10): sc-376531 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Is knocked down by siRNAs to RBM39/CaperUsed at 1:1000 dilution for Western blot of A549 and 293T lysates. The mouse monoclonal antibody detected endogenous protein well, but in addition to 64 kDa band there is a second band around 72 kDa of almost equal intensity. Both were reduced with siRNAs to RBM39.
Date published: 2019-01-03
Rated 1 out of 5 by from poor resultA poor WB result obtaining from the anti-Caper antibody.
Date published: 2018-10-03
Rated 5 out of 5 by from Good antibody for western blotI have used this antibody for WB. It works very well.
Date published: 2018-02-26
Rated 5 out of 5 by from Excellent sensitivity and selectivityExcellent sensitivity and selectivity.
Date published: 2015-11-15
Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclearProduced beautiful immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Satisfactory Western Blot data of Caper expressionSatisfactory Western Blot data of Caper expression in HeLa nuclear extract. -SCBT QC
Date published: 2013-02-28
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Caper 抗体 (G-10) is rated 4.2 out of 5 by 6.
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