Date published: 2025-9-7
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Calregulin Antibody (A-9) - Western Blotting - Image 396098
钙调素抗体 (A-9) HRP: sc-166837 HRP. 直接 Western 印迹分析 HeLa (A), MCF7 (B), HL-60 (C), A-431 (D), Jurkat (E) 和 Hep G2 (F) 全细胞裂解液中 Calregulin 的表达.
钙调素抗体 (A-9) HRP: sc-166837 HRP. 直接 Western 印迹分析 HeLa (A), MCF7 (B), HL-60 (C), A-431 (D), Jurkat (E) 和 Hep G2 (F) 全细胞裂解液中 Calregulin 的表达.

Calregulin 抗体 (A-9): sc-166837

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说明书
  • Calregulin抗体(A-9)是小鼠单克隆IgG1 κ, 在21篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Calregulin的氨基酸248-417
  • 抗-Calregulin 抗体 (A-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Calregulin
  • 抗Calregulin抗体（A-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Calregulin (A-9): sc-166837的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Calregulin Antibody (A-9) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Calregulin Antibody (A-9) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Calregulin抗体（A-9）是一种IgG1κ小鼠单克隆Calregulin抗体（也称为Calregulin抗体或Calreticulin抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的Calregulin。Calregulin抗体（A-9）可作为非偶联抗Calregulin抗体形式，以及多种偶联形式的抗Calregulin抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Calnexin和calregulin（也称为calreticulin）是钙结合蛋白，定位于内质网，Calnexin定位于膜，calregulin定位于内腔。Calnexin是一种I型膜蛋白，与内质网中新合成的糖蛋白相互作用。它可能在协助蛋白质组装和保留内质网中未组装蛋白质亚基方面发挥作用。 Calregulin具有低亲和力和高亲和力的钙结合位点。 Calnexin和calregulin对于内质网中糖蛋白的成熟都很重要，似乎与许多相同的蛋白质结合。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Calregulin 抗体 (A-9) 参考文献:

  1. 健康和疾病中的钙网蛋白信号传导。  |  Wang, WA., et al. 2012. Int J Biochem Cell Biol. 44: 842-6. PMID: 22373697
  2. 钙网蛋白在癌症生物学中的功能作用。  |  Lu, YC., et al. 2015. Biomed Res Int. 2015: 526524. PMID: 25918716
  3. 骨髓增殖性肿瘤中的钙网蛋白体细胞突变。  |  Imai, M., et al. 2017. Int J Hematol. 105: 743-747. PMID: 28470469
  4. 钙网蛋白作为神经系统疾病药理靶点的证据不断发展。  |  Kotian, V., et al. 2019. ACS Chem Neurosci. 10: 2629-2646. PMID: 31017385
  5. 骨髓增生性肿瘤中的钙网织蛋白突变。  |  Shide, K. 2021. Int Rev Cell Mol Biol. 365: 179-226. PMID: 34756244
  6. 作为癌症标志物和治疗靶点的钙网织蛋白。  |  Zhang, M., et al. 2023. Clin Exp Med. 23: 1393-1404. PMID: 36335525
  7. 钙调蛋白突变对细胞转化体和免疫的影响。  |  Desikan, H., et al. 2023. J Cell Mol Med. 27: 1032-1044. PMID: 36916035
  8. Calreticulin和JAK2V617F驱动突变会诱发骨髓增殖性肿瘤中不同的有丝分裂缺陷。  |  Holl, K., et al. 2024. Sci Rep. 14: 2810. PMID: 38308077
  9. 溶酶体降解针对骨髓增殖性肿瘤中的突变型钙网蛋白和血小板生成素受体。  |  Kaur, A., et al. 2024. Blood Adv. 8: 3372-3387. PMID: 38640435
  10. 单克隆抗体 INCA033989 选择性靶向突变的钙网蛋白，抑制了 MPN 的致癌功能。  |  Reis, ES., et al. 2024. Blood. 144: 2336-2348. PMID: 39255409

订购信息

产品名称产品编号规格价格数量收藏夹

Calregulin 抗体 (A-9)

sc-166837
200 µg/ml
$316.00

Calregulin (A-9): m-IgG Fc BP-HRP 套装

sc-529065
200 µg Ab; 10 µg BP
$354.00

Calregulin (A-9): m-IgGκ BP-HRP 套装

sc-521765
200 µg Ab, 40 µg BP
$354.00

Calregulin (A-9): m-IgG1 BP-HRP 套装

sc-543298
200 µg Ab; 20 µg BP
$354.00

Calregulin 抗体 (A-9) AC

sc-166837 AC
500 µg/ml, 25% agarose
$416.00

Calregulin 抗体 (A-9) HRP

sc-166837 HRP
200 µg/ml
$316.00

Calregulin 抗体 (A-9) FITC

sc-166837 FITC
200 µg/ml
$330.00

Calregulin 抗体 (A-9) PE

sc-166837 PE
200 µg/ml
$343.00

Calregulin 抗体 (A-9) Alexa Fluor® 488

sc-166837 AF488
200 µg/ml
$357.00

Calregulin 抗体 (A-9) Alexa Fluor® 546

sc-166837 AF546
200 µg/ml
$357.00

Calregulin 抗体 (A-9) Alexa Fluor® 594

sc-166837 AF594
200 µg/ml
$357.00

Calregulin 抗体 (A-9) Alexa Fluor® 647

sc-166837 AF647
200 µg/ml
$357.00

Calregulin 抗体 (A-9) Alexa Fluor® 680

sc-166837 AF680
200 µg/ml
$357.00

Calregulin 抗体 (A-9) Alexa Fluor® 790

sc-166837 AF790
200 µg/ml
$357.00

I want to detect calreticulin on the cell surface of mouse cells by a conjugated antibody. There are two choices for this aim, conjugated Calregulin (A-9) and (F-4). which one of them, (A-9) or (F-4), is more suitable for cell surface calreticulin?

Asked by: sjafari
Thank you for your question. Both antibodies are recommended for the detection of murine calregulin by IF or IHC(P). For Calregulin Antibody (F-4): sc-373863 there are two publications showing cell surface staining: PMID 27789271 and PMID 29324305. For further questions or to arrange for a free sample of one or both of the antibodies please contact technical service directly.
Answered by: SCBT Heidelberg Support
Date published: 2019-12-10

I am using Calregulin (A-9): sc-166837 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Wonderful antibodyI recently tested 2 Calreticulin/Calregulin Sc-373863 and Sc-166837 antibodies in human cell lines and both of them turned equally good. Calregulin gives approximately 55kDa band and expressed widely in human tissues. HEP-G2 is a liver cancer cell line and HEK293 was derived form embryonic kidney carcinoma but the later reported to express neuronal proteins and may be neuronal in origin. Highly recomend this antibody, See my blots.
Date published: 2017-09-13
Rated 5 out of 5 by from Very Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-02-07
Rated 5 out of 5 by from Published WB data using murine MC3T3Published WB data using murine MC3T3-E1 cell lysates. -SCBT Publication Review
Date published: 2015-06-04
Rated 5 out of 5 by from Strong bands in western blot analysis ofStrong bands in western blot analysis of Calregulin expression in HeLa and MCF7 whole cell lysates. -SCBT QC
Date published: 2015-02-23
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmicProduced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human thyroid gland tissue. -SCBT QC
Date published: 2014-08-13
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Calregulin 抗体 (A-9) is rated 5.0 out of 5 by 5.
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