Calponin 3抗体 (A-2) 推荐用于 mouse, rat 和 human 来源的Calponin 3 WB, IP, IF, IHC(P) 和 ELISA检测

Calponin 3 抗体 (A-2): sc-271188

Calponin 3抗体 (A-2) is rated 4.8 out of 5 by 4.
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说明书

    • Calponin 3抗体(A-2)是小鼠单克隆IgG1 κ, 在2篇文献中引用,规格为200 µg/ml
    • raised against amino acids 275-329 mapping at the C-terminus of Calponin 3 of human and rat origin
    • 抗-Calponin 3 抗体 (A-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Calponin 3
    • 抗-Calponin 3 抗体 (A-2) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Calponin 3 (A-2): sc-271188免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • 对于Calponin 3 抗体 (A-2)的WB and IHC(P)应用,m-IgGκ BP-HRP 是首选的二抗检测试剂。现在该试剂和Calponin 3 抗体 (A-2)组合成套装提供(参见下面的订购信息)。对于其他m-IgGκ BP 偶联产品,参见我们小鼠IgG 结合蛋白的完整列表
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I am using Calponin 3 (A-2): sc-271188 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from GREAT RESULTS! I RECENTLY TRIED THIS ANTIBODY ON MOUSE AORTA(FORMALIN FIXED- PARAFFIN EMBEDDED) FOR IF. I USED THE ANTIBODY IN 1:200 DILUTION CD31 GREEN CALP3 RED
Date published: 2017-01-24
Rated 5 out of 5 by from Strong bands in western blot analysis of Strong bands in western blot analysis of Calponin 3 expression in KNRK and SK-N-SH whole cell lysates. -SCBT QC
Date published: 2015-07-06
Rated 5 out of 5 by from WB: positive results in human colon tissue extract WB: positive results in human colon tissue extract. -SCBT QC
Date published: 2015-06-04
Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic staining Beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-04-21
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