Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Calpain 2 CRISPR/Cas9 KO Plasmid (h): sc-401113
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Calpain 2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Calpain 2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Calpain 2 Antibody (E-10): sc-373966
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calpain 2 CRISPR/Cas9 KO Plasmid (h) | sc-401113 | 20 µg | $397.00 |
Overview
CAPN2 encodes calpain 2, a ubiquitous Ca²⁺-dependent cysteine protease that performs limited proteolysis of cytoskeletal and signaling proteins to remodel cellular architecture. Calpain 2 activity contributes to focal adhesion turnover, membrane trafficking, and modulation of kinase and phosphatase signaling, intersecting with pathways controlling migration, survival, and stress responses. Through regulated cleavage of substrates involved in cell cycle progression and apoptosis, CAPN2 helps tune proteostasis and adaptive signaling during inflammation and tissue remodeling. Dysregulated calpain 2 activity has been associated with aberrant motility and invasive phenotypes, neurodegenerative processes linked to excitotoxic calcium influx, and altered responses to oxidative or mechanical stress.
Calpain 2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CAPN2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CAPN2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CAPN2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Calpain 2 protein expression.
This CRISPR knockout system enables efficient generation of CAPN2-deficient cell models for investigation of Calpain 2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CAPN2 exon(s) critical for Calpain 2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CAPN2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Calpain 2 CRISPR/Cas9 KO Plasmid (h) and Calpain 2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CAPN2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Calpain 2 HDR Plasmid (h) and Calpain 2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CAPN2 homology arms to support homology-directed repair at defined CAPN2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.