Calpain 2抗体 (E-10) 推荐用于 mouse, rat 和 human 来源的Calpain 2 precursor WB, IP, IF, IHC(P) 和 ELISA检测

Calpain 2 抗体 (E-10): sc-373966

Calpain 2抗体 (E-10) is rated 4.8 out of 5 by 10.
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说明书

    • Calpain 2抗体(E-10)是小鼠单克隆IgG1 κ, 在10篇文献中引用,规格为200 µg/ml
    • 特异性抗原位于human物种的Calpain 2的N-terminus的氨基酸2-27之间
    • 抗-Calpain 2 抗体 (E-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Calpain 2 precursor
    • 抗-Calpain 2 抗体 (E-10) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Calpain 2 (E-10): sc-373966免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Which tissue or cell can be a positive control for calpain2 IHC ?

Asked by: hexl7
Thank you for your question. Bronchus tissue, fallopian tube, gall bladder or rectum tissues are all good positive controls for this protein for IHC.
Answered by: Tech Service
Date published: 2019-04-22

Was the antibody immunized against isoform 1 or 2 of calpain-2? Isoform 2 lacks the first 78 amino acids of isoform 1.

Asked by: Claudia_NeuroGU
Thank you for your question. Calpain 2 Antibody (E-10): sc-373966 is specific for an epitope mapping between amino acids 2-27 at the N-terminus of Calpain 2 of human origin. The immunogenic sequence is AGIAAKLAK DREAAEGLGS HDRAIKY. Since amino acids 1-78 are missing in isoform P17655-2, this isoform is not recognized by Calpain 2 Antibody (E-10): sc-373966 or Calpain 2 Antibody (F-11): sc-373967, which has been raised against the same range of amino acids.
Answered by: Technical Support Europe
Date published: 2019-01-14

What application is the blocking peptide sc-373966 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Strong Bands Clean and strong bands detected using human cardiac stem cell lysate (30ug protein). Worked at 1:500 primary dilution with 1:1000 anti-mouse secondary.
Date published: 2018-02-20
Rated 5 out of 5 by from staining image The test indicates the effectiveness of the results of image enhancement. Experiments show that it is an efficient method
Date published: 2017-10-19
Rated 5 out of 5 by from Strong signals with clean background I got the sample for western blotting using the lysates from rat cultured neurons and mouse brains, and I got strong calpain 2 signals with the clean background. I use PVDF membrane and the antibody is 1:5000 diluted in 3% milk/TBST.
Date published: 2017-09-26
Rated 5 out of 5 by from Great I got a free sample for test, and it works very well
Date published: 2017-09-07
Rated 4 out of 5 by from suitable for IF suitable for formaldehyde-fixed breast cancer cells MCF-7 by IF
Date published: 2017-08-26
Rated 5 out of 5 by from Robust and clean Antibody works pretty well and performed western blot, band is pretty clear and strong.
Date published: 2017-05-23
Rated 5 out of 5 by from strong bands Used at 1:500 to detect Calpain 2 in U87MG cells,works good,single specific band detected,staining data very strong.
Date published: 2017-02-24
Rated 5 out of 5 by from Well-rounded antibody for Calpain 2 Excellent antibody for Calpain 2, strong and specific, giving solid bands in Western blot and clean clear staining in IHC. Great choice!
Date published: 2017-01-31
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