Date published: 2025-9-5

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CagA 抗体 (A-10): sc-28368

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说明书
  • CagA 抗体 A-10 是小鼠单克隆 IgG2a κ,CagA抗体, 在54篇文献中引用,规格为200 µg/ml
  • 免疫H. pylori物种的CagA的氨基酸1-300
  • CagA 抗体 (A-10) 推荐用于WB, IP 和 ELISA,检测H. pylori 来源的 CagA
  • 抗CagA抗体(A-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CagA (A-10): sc-28368免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是CagA Antibody (A-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与CagA Antibody (A-10) 打包提供(请参阅下面的订购信息)。

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関連項目

CagA抗体(A-10)是一种鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和酶联免疫吸附测定(ELISA)检测幽门螺杆菌的CagA蛋白。CagA抗体(A-10)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。CagA是幽门螺杆菌的主要致病因子,在胃溃疡和胃癌等胃病的发病机理中起着关键作用。CagA一旦注入宿主细胞,就会发生磷酸化,这是其发挥功能的关键,因为磷酸化后的CagA会与宿主细胞的多种信号通路相互作用。这种翻译后修饰使CagA能够操纵宿主细胞的行为,导致细胞形态改变、肌动蛋白细胞骨架重组以及正常细胞进程的中断。CagA诱导这些改变的能力与致癌潜力有关,使其成为胃癌发生的重要因素。了解CagA的作用和变化不仅有助于揭示幽门螺杆菌的发病机理,而且有助于确定治疗幽门螺杆菌相关疾病的潜在靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CagA 抗体 (A-10) 参考文献:

  1. 幽门螺旋杆菌感染的机制:细菌因素。  |  McGee, DJ. and Mobley, HL. 1999. Curr Top Microbiol Immunol. 241: 155-80. PMID: 10087661
  2. 幽门螺杆菌相关的胃部病理。  |  Konturek, PC., et al. 1999. J Physiol Pharmacol. 50: 695-710. PMID: 10695552
  3. 疾病特异性幽门螺旋杆菌毒力因子:尚未兑现的承诺。  |  Graham, DY. and Yamaoka, Y. 2000. Helicobacter. 5 Suppl 1: S3-9; discussion S27-31. PMID: 10828748
  4. 接触幽门螺旋杆菌后引起的细胞反应  |  Censini, S., et al. 2001. Curr Opin Microbiol. 4: 41-6. PMID: 11173032
  5. 幽门螺旋杆菌的致病因子。  |  Dundon, WG., et al. 2001. Int J Med Microbiol. 290: 647-58. PMID: 11310443
  6. 感染 CagA+ 幽门螺旋杆菌的患者患萎缩性胃炎的风险增加。  |  Sande, N., et al. 2001. Scand J Gastroenterol. 36: 928-33. PMID: 11521982
  7. 幽门螺杆菌CagA的分子解剖学和致病作用是胃癌发生的基础。  |  Takahashi-Kanemitsu, A., et al. 2020. Cell Mol Immunol. 17: 50-63. PMID: 31804619
  8. 幽门螺杆菌 CagA 可诱发 BRCAness,从而导致基因组不稳定,这可能是细菌性胃癌发生的基础。  |  Imai, S., et al. 2021. Cell Host Microbe. 29: 941-958.e10. PMID: 33989515
  9. CagA+幽门螺杆菌、非CagA-幽门螺杆菌、感染通过外泌体介导的ROS形成损害内皮功能。  |  Xia, X., et al. 2022. Front Cardiovasc Med. 9: 881372. PMID: 35433874
  10. 幽门螺杆菌毒性蛋白CagA诱导的信号通路综述。  |  Wang, H., et al. 2023. Front Cell Infect Microbiol. 13: 1062803. PMID: 37124036
  11. 幽门螺杆菌CagA介导的乙醚脂生物合成促进胃癌对铁死亡的敏感性。  |  Peng, Y., et al. 2024. Exp Mol Med. 56: 441-452. PMID: 38383581
  12. Lumisterol由CYP11A1代谢:发现新途径。  |  Zhang, Z., et al. 2024. Int J Biol Sci. 20: 4007-4028. PMID: 39113698

订购信息

产品名称产品编号规格价格数量收藏夹

CagA 抗体 (A-10)

sc-28368
200 µg/ml
$316.00

CagA (A-10): m-IgG Fc BP-HRP 套装

sc-528437
200 µg Ab; 10 µg BP
$354.00

CagA (A-10): m-IgGκ BP-HRP 套装

sc-520812
200 µg Ab, 40 µg BP
$354.00

CagA (A-10): m-IgG2a BP-HRP 套装

sc-547097
200 µg Ab; 10 µg BP
$354.00

CagA 抗体 (A-10) AC

sc-28368 AC
500 µg/ml, 25% agarose
$416.00

CagA 抗体 (A-10) HRP

sc-28368 HRP
200 µg/ml
$316.00

CagA 抗体 (A-10) FITC

sc-28368 FITC
200 µg/ml
$330.00

CagA 抗体 (A-10) PE

sc-28368 PE
200 µg/ml
$343.00

CagA 抗体 (A-10) Alexa Fluor® 488

sc-28368 AF488
200 µg/ml
$357.00

CagA 抗体 (A-10) Alexa Fluor® 546

sc-28368 AF546
200 µg/ml
$357.00

CagA 抗体 (A-10) Alexa Fluor® 594

sc-28368 AF594
200 µg/ml
$357.00

CagA 抗体 (A-10) Alexa Fluor® 647

sc-28368 AF647
200 µg/ml
$357.00

CagA 抗体 (A-10) Alexa Fluor® 680

sc-28368 AF680
200 µg/ml
$357.00

CagA 抗体 (A-10) Alexa Fluor® 790

sc-28368 AF790
200 µg/ml
$357.00

We recently bought the CagA Antibody (A-10) Alexa Fluor® 488 for FCM and IF starting with a 1:200 dilution and no results. Any protocol or conditions of permeabilization for this antibody?

Asked by: ale9
Thank you for your question. We recommend a starting dilution of 1:50 for IF using this antibody. Please contact your local distributor for assistance regarding your results, we are happy to help troubleshoot this with you.
Answered by: Tech Service
Date published: 2019-01-14

Guten Morgen, ich hätte gerne das 10 µg GRATIS Sample vom CagA (A-10): sc-28368 für unsere AG Mikrobiologie. Vielen Dank.

Asked by: Biosciences
Vielen Dank für Ihre Frage. Wenn Sie Interesse daran haben, ein 10 µg Testsample CagA (A-10): sc-28368 zu erhalten, kontaktieren Sie unseren technischen Service unter, per Telefon oder über unseren. Thank you for your inquiry to receive a test aliquot of CagA (A-10): sc-28368. If you have an interest in testing a free 10 µg sample of CagA (A-10): sc-28368 please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

which species could this antibody  react with ?

Asked by: xiaoshucong
Thank you for your question. CagA Antibody (A-10): sc-28368 is recommended for detection of Cytotoxin associated gene A (also known as CagA) of Helicobacter pylori origin. The antibody reacts with the H. pylori strain of bacteria.
Answered by: Technical Support
Date published: 2017-03-02

What secondary is best to use with CagA (A-10): sc-28368?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with CagA (A-10): sc-28368, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-17
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Rated 5 out of 5 by from Good results in WBGood CagA antibody for WB. Good signals could be detected with 1:750 dilution. It gives quite specific bands, with hardly any background noise!
Date published: 2018-08-14
Rated 5 out of 5 by from 抗体不错,老板满意性价比高。。品质还可以,老板对实验满意。如果能在便宜点,就更好了,就是多抗现在抗体很少了。希望能有更多的好产品
Date published: 2018-05-19
Rated 5 out of 5 by from 这个抗体不错,老板喜欢老师觉得santan的部分抗体还是性价比比较高的。。价格也亲民。。就是有的时候偶尔品质不稳定。需要多沟通,加强品质。
Date published: 2018-05-11
Rated 5 out of 5 by from strong signalstrong signal 1:800 diluted the antibody, great result and very specific.
Date published: 2017-08-19
Rated 5 out of 5 by from excellent antibodyA excellent antibody for Western blots. I have diluted this antibody 1:500 and still get a very high, specific signal from HepG2 cells. The antibody solution can be decreased at 1:1000.
Date published: 2017-07-23
Rated 5 out of 5 by from Best resultDetect human colon tissue by WB,1:400 dilution get best result.
Date published: 2017-05-27
Rated 5 out of 5 by from IF in Huh7 cellsWorks well in immunostaining, CagA was transiently expressed in Huh7 cells
Date published: 2017-04-25
Rated 5 out of 5 by from Well cited CagA antibodyCagA (A-10) is well cited for WB and IHC applications. Western blot (WB) in-house testing performed on recombinant CagA fusion protein tagged to GST.
Date published: 2017-01-13
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CagA 抗体 (A-10) is rated 5.0 out of 5 by 13.
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