CAD Antibody (H-1): sc-5295

CAD Antibody (H-1) is a mouse monoclonal IgG2b antibody that detects CAD protein in human samples through various applications including western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and enzyme-linked immunosorbent assay. CAD, also known as caspase-activated deoxyribonuclease, plays a crucial role in the apoptotic process by facilitating DNA degradation, which is essential for the orderly dismantling of cellular components during programmed cell death. CAD protein is activated when caspase-3 cleaves ICAD, allowing CAD to translocate to the nucleus where CAD initiates the degradation of DNA, thereby contributing to the morphological changes associated with apoptosis. CAD regulation is vital, as improper activation can lead to pathological conditions, including cancer and neurodegenerative diseases, highlighting the importance of understanding CAD function and regulation in cellular processes. CAD monoclonal antibody (H-1) provides a reliable tool for researchers studying apoptosis and its implications in various diseases, enabling the exploration of therapeutic targets and the underlying mechanisms of cell death.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CAD Antibody (H-1) References:

1. Detection of DNA cleavage in apoptotic cells.  |  Kaufmann, SH., et al. 2000. Methods Enzymol. 322: 3-15. PMID: 10914000
2. Possible involvement of cyclophilin B and caspase-activated deoxyribonuclease in the induction of chromosomal DNA degradation in TCR-stimulated thymocytes.  |  Nagata, T., et al. 2000. J Immunol. 165: 4281-9. PMID: 11035062
3. Caspase-3 and caspase-activated deoxyribonuclease are associated with testicular germ cell apoptosis resulting from reduced intratesticular testosterone.  |  Kim, JM., et al. 2001. Endocrinology. 142: 3809-16. PMID: 11517157
4. Induction of caspase-activated deoxyribonuclease activity after focal cerebral ischemia and reperfusion.  |  Luo, Y., et al. 2002. J Cereb Blood Flow Metab. 22: 15-20. PMID: 11807389
5. Surviving apoptosis.  |  Vaughan, AT., et al. 2002. Apoptosis. 7: 173-7. PMID: 11865202
6. Endonuclease activation and chromosomal DNA fragmentation during apoptosis in leukemia cells.  |  Yoshida, A., et al. 2006. Int J Hematol. 84: 31-7. PMID: 16867899
7. Apoptotic DNA fragmentation may be a cooperative activity between caspase-activated deoxyribonuclease and the poly(ADP-ribose) polymerase-regulated DNAS1L3, an endoplasmic reticulum-localized endonuclease that translocates to the nucleus during apoptosis.  |  Errami, Y., et al. 2013. J Biol Chem. 288: 3460-8. PMID: 23229555
8. Chromatin collapse during caspase-dependent apoptotic cell death requires DNA fragmentation factor, 40-kDa subunit-/caspase-activated deoxyribonuclease-mediated 3'-OH single-strand DNA breaks.  |  Iglesias-Guimarais, V., et al. 2013. J Biol Chem. 288: 9200-15. PMID: 23430749
9. Small-molecule targeted therapies induce dependence on DNA double-strand break repair in residual tumor cells.  |  Ali, M., et al. 2022. Sci Transl Med. 14: eabc7480. PMID: 35353542
10. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD.  |  Enari, M., et al. 1998. Nature. 391: 43-50. PMID: 9422506

The preferred CAD antibody is CAD Antibody (F-11): sc-374067.