Date published: 2025-9-8

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C23 (Nucleolin) 抗体 (H-6): sc-55486

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说明书
  • C23 (Nucleolin)抗体(H-6)是小鼠单克隆IgG2a κ, 在35篇文献中引用,规格为200 µg/ml
  • 免疫human物种的C23的氨基酸271-520
  • 抗-C23 (Nucleolin) 抗体 (H-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 C23
  • 抗C23 (Nucleolin)抗体(H-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取C23 (Nucleolin) (H-6): sc-55486免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2a BP-HRP 是 C23 (Nucleolin) 抗体 (H-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与C23 (Nucleolin) 抗体 (H-6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

C23 抗体 (H-6) 是一种小鼠单克隆 IgG2a kappa 轻链抗体,可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、石蜡包埋切片免疫组织化学 (IHCP) 和酶联免疫吸附试验 (ELISA) 检测小鼠、大鼠和人样本中的 C23。C23 抗体 (H-6) 有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。C23 又称核仁蛋白(NCL),是一种重要的真核细胞核极磷蛋白,在核糖体合成和成熟过程中发挥着重要作用,是所有活细胞中蛋白质合成所必需的。NCL (C23) 主要位于核仁的致密纤维状区域,含有四个 RNA 结合域,有助于在核糖体生物发生过程中与前核糖体 RNA 相互作用。NCL (C23) 的定位和功能对 RNA 处理、核糖体基因转录和核糖体成分的核仁靶向至关重要。NCL (C23) 与包括核小体蛋白 B23 在内的几种蛋白相互作用,同时被 Cdc2 和酪蛋白激酶 II 磷酸化,导致核小体向细胞质转位,凸显了其在细胞中的动态作用。NCL (C23) 参与人类端粒酶逆转录酶亚基(TERT)的定位调控,强调了其在细胞衰老和癌症生物学中的重要性,这使得抗 C23 抗体 (H-6) 在研究核糖体生物生成和相关途径方面具有重要价值。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

C23 抗体 (H-6)

sc-55486
200 µg/ml
$316.00

C23 (Nucleolin) (H-6): m-IgG Fc BP-HRP 套装

sc-525626
200 µg Ab; 10 µg BP
$354.00

C23 (Nucleolin) (H-6): m-IgG2a BP-HRP 套装

sc-546342
200 µg Ab; 10 µg BP
$354.00

C23 (Nucleolin) 抗体 (H-6) AC

sc-55486 AC
500 µg/ml, 25% agarose
$416.00

C23 (Nucleolin) 抗体 (H-6) HRP

sc-55486 HRP
200 µg/ml
$316.00

C23 (Nucleolin) 抗体 (H-6) FITC

sc-55486 FITC
200 µg/ml
$330.00

C23 (Nucleolin) 抗体 (H-6) PE

sc-55486 PE
200 µg/ml
$343.00

C23 (Nucleolin) 抗体 (H-6) Alexa Fluor® 488

sc-55486 AF488
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (H-6) Alexa Fluor® 546

sc-55486 AF546
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (H-6) Alexa Fluor® 594

sc-55486 AF594
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (H-6) Alexa Fluor® 647

sc-55486 AF647
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (H-6) Alexa Fluor® 680

sc-55486 AF680
200 µg/ml
$357.00

C23 (Nucleolin) 抗体 (H-6) Alexa Fluor® 790

sc-55486 AF790
200 µg/ml
$357.00

I am using C23 (H-6): sc-55486 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from buon anticorpoquesto anticorpo dà un buon segnale, forte e specifico
Date published: 2020-10-16
Rated 4 out of 5 by from Works well for ICCUsed for immunocytochemistry on Hela cells using conventional protocol.
Date published: 2018-06-20
Rated 4 out of 5 by from work well by WB and IF (1/200) on human cancerwork well by WB and IF (1/200) on human cancer cells
Date published: 2016-05-06
Rated 5 out of 5 by from Produced exceptional immunoperoxidase nuclearProduced exceptional immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Strong band in WB in CCRFStrong band in WB in CCRF-CEM whole cell lysate. -SCBT QC
Date published: 2015-04-11
Rated 5 out of 5 by from ClearClear, crisp band obtained in WB in 293T whole cell lysate. -SCBT QC
Date published: 2015-03-11
Rated 5 out of 5 by from Published data for WB in human LLCPublished data for WB in human LLC-PK1 cells. -SCBT Publication Review
Date published: 2015-02-20
Rated 5 out of 5 by from Published data for IF inhuman foreskin fibroblastsPublished data for IF inhuman foreskin fibroblasts (HFFs). -SCBT Publication Review
Date published: 2015-01-28
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C23 (Nucleolin) 抗体 (H-6) is rated 4.7 out of 5 by 10.
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