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Myc Antibody (9E11) - Western Blotting - Image 380167
c-Myc 抗体 (9E11): sc-47694. Western 印迹分析 NIH/3T3 全细胞裂解液中 c-Myc 的表达.
c-Myc 抗体 (9E11): sc-47694. Western 印迹分析 NIH/3T3 全细胞裂解液中 c-Myc 的表达.

Myc 抗体 (9E11): sc-47694

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说明书
  • Myc抗体(9E11)是小鼠单克隆IgG2a κ, 在98篇文献中引用，规格为200 µg/ml
  • 针对氨基酸408-420的抗原，位于c-Myc的C-terminal结构域内，来源于human
  • 抗-Myc 抗体 (9E11) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human 和monkey 来源的 c-Myc p67 and c-Myc tagged fusion proteins
  • 抗Myc抗体（9E11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联生物素 用于WB, IHC(P) 和 ELISA；偶联TRITC 用于IF, IHC(P) 和 FCM
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-47694 X, 200 µg/0.1 ml)
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是Myc Antibody (9E11) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Myc Antibody (9E11) 打包提供（请参阅下面的订购信息）。
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関連項目

额外Myc抗体，包括c-Myc和N-Myc

c-Myc抗体（9E11）是一种小鼠单克隆IgG2a kappa轻链抗体，针对人类c-Myc的氨基酸408-420。c-Myc小鼠单克隆抗体（9E11）识别该区域内的表位，并检测来自小鼠、大鼠和人类来源的c-Myc标记融合蛋白。抗c-Myc单克隆抗体（9E11）非常适合用于对石蜡包埋组织进行蛋白质印迹、免疫沉淀、免疫荧光和免疫组织化学分析。c-Myc（9E11）抗体有非结合型和多种结合型，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®结合型，可满足各种实验需求。c-Myc蛋白由8q24染色体上的MYC原癌基因编码，是一种核转录因子，在调节细胞增殖、分化及凋亡方面起着至关重要的作用。c-Myc在C端区域包含一些重要的结构基序，如碱基区、螺旋-环-螺旋区以及亮氨酸拉链结构域，这些结构域对于DNA结合以及与Max蛋白的二聚化至关重要。c-Myc/Max异源二聚体与目标基因启动子中的特定E-box序列（CACGTG）结合，激活转录并促进细胞周期进展。c-Myc基因的过度表达或扩增是多种人类癌症的共同特征，包括伯基特淋巴瘤、神经母细胞瘤以及肺癌、乳腺癌和结肠癌，导致细胞无序生长和肿瘤发展。Max可以与其他基本螺旋-环-螺旋亮氨酸拉链蛋白（如Mad1和Mxi1）形成异二聚体；这些复合物可以通过招募共抑制剂（如mSin3）来抑制转录，从而抵消c-Myc的活性。了解涉及c-Myc的复杂调节机制对于癌症研究和靶向疗法的开发至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Myc 抗体 (9E11) 参考文献:

  1. MicroRNAs作为c-MYC功能的调节剂和中介。  |  Jackstadt, R. and Hermeking, H. 2015. Biochim Biophys Acta. 1849: 544-53. PMID: 24727092
  2. SCP1 通过使 c-Myc Ser62 去磷酸化来调节 c-Myc 的稳定性和功能。  |  Wang, W., et al. 2016. Oncogene. 35: 491-500. PMID: 25893300
  3. c-MYC抑制可削弱多形性胶质母细胞瘤的缺氧反应。  |  Mongiardi, MP., et al. 2016. Oncotarget. 7: 33257-71. PMID: 27119353
  4. 调控p53和c-Myc信号的非编码RNA。  |  Mei, Y. and Wu, M. 2016. Adv Exp Med Biol. 927: 337-65. PMID: 27376742
  5. c-Myc 失调导致依赖 mRNA 封端酶。  |  Lombardi, O., et al. 2016. Oncotarget. 7: 82273-82288. PMID: 27756891
  6. Gfi1上调c-Myc表达并促进c-Myc驱动的细胞增殖。  |  Zhang, Y. and Dong, F. 2020. Sci Rep. 10: 17115. PMID: 33051558
  7. 去泛素化酶OTUB1通过去泛素化c-MYC调节阿霉素诱导的心脏毒性。  |  Xu, F., et al. 2024. Cell Signal. 113: 110937. PMID: 37871668
  8. CRL2KLHDC3和CRL1Fbxw7协同介导c-Myc降解。  |  Motomura, S., et al. 2024. Oncogene. 43: 1917-1929. PMID: 38698266
  9. HIF1、c-Myc 和 p53 在胶质瘤细胞中的代谢作用。  |  Trejo-Solís, C., et al. 2024. Metabolites. 14: PMID: 38786726
  10. GRP78 抑制剂 YUM70 可上调 4E-BP1，抑制 c-MYC 表达和致癌 c-MYC 肿瘤的存活能力。  |  Yamamoto, V., et al. 2024. Neoplasia. 55: 101020. PMID: 38991376

订购信息

产品名称产品编号规格价格数量收藏夹

Myc 抗体 (9E11)

sc-47694
200 µg/ml
$322.00

Myc (9E11): m-IgG Fc BP-HRP 套装

sc-528507
200 µg Ab; 10 µg BP
$361.00

Myc (9E11): m-IgGκ BP-HRP 套装

sc-520901
200 µg Ab, 40 µg BP
$361.00

Myc (9E11): m-IgG2a BP-HRP 套装

sc-547113
200 µg Ab; 10 µg BP
$361.00

Myc 抗体 (9E11) X

sc-47694 X
200 µg/0.1 ml
$322.00

Myc 抗体 (9E11) AC

sc-47694 AC
500 µg/ml, 25% agarose
$424.00

Myc 抗体 (9E11) HRP

sc-47694 HRP
200 µg/ml
$322.00

Myc 抗体 (9E11) FITC

sc-47694 FITC
200 µg/ml
$336.00

Myc 抗体 (9E11) PE

sc-47694 PE
200 µg/ml
$349.00

Myc 抗体 (9E11) Alexa Fluor® 488

sc-47694 AF488
200 µg/ml
$364.00

Myc 抗体 (9E11) Alexa Fluor® 546

sc-47694 AF546
200 µg/ml
$364.00

Myc 抗体 (9E11) Alexa Fluor® 594

sc-47694 AF594
200 µg/ml
$364.00

Myc 抗体 (9E11) Alexa Fluor® 647

sc-47694 AF647
200 µg/ml
$364.00

Myc 抗体 (9E11) Alexa Fluor® 680

sc-47694 AF680
200 µg/ml
$364.00

Myc 抗体 (9E11) Alexa Fluor® 790

sc-47694 AF790
200 µg/ml
$364.00

Myc 抗体 (9E11) B

sc-47694 B
200 µg/ml
$326.00

Myc 抗体 (9E11) TRITC

sc-47694 TRITC
200 µg/ml
$326.00

What is the band size expected in western blotting when using 9E11 c-Myc antibody?

Asked by: priyanka2pdm
Thank you for your question. The expected molecular weight for endogenous c-myc is ~67kDa.
Answered by: Tech Service
Date published: 2019-02-12

When should the TransCruz reagent sc-47694 X be used instead of the standard form sc-47694?

Asked by: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 10
Date published: 2017-01-13

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with c-Myc (9E11): sc-47694?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-12
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Rated 5 out of 5 by Martina24 from Great signalrimary antibody c-Myc (9E11) sc-47694 was used diluted 1:1000 according to the manufacturer's recommendations (for Western Blotting dilution range 1:100-1:1000), in Milk 5%. Membranes of PVDF have been incubated overnight at 4°C and then they have been probed for 1h at RT with secondary antibodies diluted 1:5000 in Milk 5%. We have tested this antibodiy three tumor cell lines: HeLa, A375 and glioblostoma primary cell line. In all the samples the recognition of the TAG was effective. The signal was clear and good, so we can conclude that the antibody has worked well. very well.
Date published: 2020-11-09
Rated 1 out of 5 by A603 from バックが高いHEK293T細胞に過剰発現したMycタグ付きのタンパク質を検出できますが、他社より感度が低く、バックグラウンドシグナルが非常に多いです。
Date published: 2020-10-15
Rated 5 out of 5 by gkyriakopoulos from Great antibodyI ordered this antibody as a sample and tested it on A549, HEK293 and HeLa cell lines and had great bands.
Date published: 2020-05-10
Rated 3 out of 5 by Tsen from Antibody reviewWorked in WB with mouse brain tissue at 1:500 dilution.
Date published: 2018-05-29
Rated 4 out of 5 by crocodile99 from knockin mouse embryo IP2ug of 9e11 works fine (slightly better than 9E10), but overall enrichment was not great compared to input.
Date published: 2017-12-27
Rated 5 out of 5 by Bill28 from Outstanding product !!Very clear western blot data;also great for IHC staining
Date published: 2017-01-30
Rated 5 out of 5 by Sweetums from Good antibody for c-MycWell-published antibody for c-Myc and c-Myc tagged fusion proteins. Strong, sensitive detection in Western blot and staining.
Date published: 2017-01-27
Rated 4 out of 5 by FL from Works in Western with human Ishikawa endometrialWorks in Western with human Ishikawa endometrial cancer cell line. -SCBT Publication Review
Date published: 2014-01-01
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Myc 抗体 (9E11) is rated 4.0 out of 5 by 9.
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