Date published: 2025-9-6
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c-Myb Antibody (D-7) - Western Blotting - Image 384071
c-Myb 抗体 (D-7) HRP: sc-74512 HRP. THP-1 (A), K-562 (B), CCRF-CEM (C), Jurkat (D) 和 MOLT-4 (E) 全细胞裂解液中 c-Myb 表达的直接 Western 印迹分析.
c-Myb 抗体 (D-7) HRP: sc-74512 HRP. THP-1 (A), K-562 (B), CCRF-CEM (C), Jurkat (D) 和 MOLT-4 (E) 全细胞裂解液中 c-Myb 表达的直接 Western 印迹分析.

c-Myb 抗体 (D-7): sc-74512

4.9(12)
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说明书
  • c-Myb 抗体 D-7 是小鼠单克隆 IgG2a κ，c-Myb抗体, 在20篇文献中引用，规格为200 µg/ml
  • 免疫human物种的c-Myb的氨基酸500-640
  • c-Myb 抗体 (D-7) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 c-Myb
  • 抗c-Myb抗体（D-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-74512 X, 200 µg/0.1 ml)
  • 关于如何获取c-Myb (D-7): sc-74512的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是c-Myb Antibody (D-7) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与c-Myb Antibody (D-7) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加Myb抗体，包括A-Myb, B-Myb, c-Myb和v-Myb

c-Myb抗体（D-7）是一种小鼠单克隆IgG2a kappa轻链抗体，用于检测c-Myb蛋白，也称为骨髓增生病毒癌基因同源物c-Myb、MYB原癌基因或c-Myb转录因子。c-Myb单克隆抗体（D-7）针对人类c-Myb的氨基酸500-640，可有效识别小鼠、大鼠和人类c-Myb蛋白，适用于多种应用，包括蛋白质印迹（WB）、免疫沉淀（ 免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）等多种应用中有效识别小鼠、大鼠和人类c-Myb蛋白。c-Myb蛋白主要定位于细胞核，作为重要的转录因子，调节造血细胞增殖、分化及存活相关基因的表达。这种核定位对于c-Myb在控制转录程序方面的作用至关重要，转录程序可维持细胞生长和分化之间的平衡，使c-Myb成为正常造血的重要组成部分，并在失调时参与致癌过程。抗c-Myb抗体（D-7 ）有非结合型和多种结合型，包括辣根过氧化物酶（HRP）、异硫氰酸荧光素（FITC）、藻红蛋白（PE）和Alexa Fluor®结合物，为各种实验设置提供了灵活性。c-Myb (D-7)抗体有助于研究c-Myb的核功能，支持对血细胞发育和相关疾病的分子机制进行深入研究。无论是研究正常的造血过程还是探索白血病发生的途径，c-Myb单克隆抗体（D-7）都是研究人员阐明Myb家族转录因子关键作用不可或缺的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

c-Myb 抗体 (D-7) 参考文献:

  1. 染色质重塑因子 Mi-2alpha 是人类 c-Myb 的新型共激活因子。  |  Saether, T., et al. 2007. J Biol Chem. 282: 13994-4005. PMID: 17344210
  2. c-Myb 是结肠隐窝祖细胞平衡的必要条件。  |  Malaterre, J., et al. 2007. Proc Natl Acad Sci U S A. 104: 3829-34. PMID: 17360438
  3. 羧基截断增加了特异性 DNA 结合，这表明了 Myb 的激活机制。  |  Ramsay, RG., et al. 1991. Oncogene. 6: 1875-9. PMID: 1923510
  4. 与 c-MYB 相比，人类 B-myb 蛋白的 DNA 结合活性和转录激活功能。  |  Mizuguchi, G., et al. 1990. J Biol Chem. 265: 9280-4. PMID: 2160970
  5. 造血染色质结构中的c-Myb结合位点。  |  Bengtsen, M., et al. 2015. PLoS One. 10: e0133280. PMID: 26208222
  6. c-myb 原癌基因编码的转录激活因子的三个功能域的划分。  |  Sakura, H., et al. 1989. Proc Natl Acad Sci U S A. 86: 5758-62. PMID: 2668947
  7. 小鼠 myb 原癌基因 cDNA 克隆的核苷酸序列。  |  Gonda, TJ., et al. 1985. EMBO J. 4: 2003-8. PMID: 2998780
  8. 转录因子 c-Myb 调节 CD8+ T 细胞干性和抗肿瘤免疫。  |  Gautam, S., et al. 2019. Nat Immunol. 20: 337-349. PMID: 30778251
  9. c-Myb调节胚胎横纹肌肉瘤细胞的肿瘤发生潜力。  |  Kaspar, P., et al. 2019. Sci Rep. 9: 6342. PMID: 31004084
  10. 小鼠髓性白血病分化过程中 myb, myc 和 fos 原癌基因的表达。  |  Gonda, TJ. and Metcalf, D. Nature. 310: 249-51. PMID: 6205276
  11. B-myb 的连续表达可以绕过 p53 诱导的 Waf1/Cip1 介导的 G1 停顿。  |  Lin, D., et al. 1994. Proc Natl Acad Sci U S A. 91: 10079-83. PMID: 7937841
  12. 检测与 c-Myb 的亮氨酸拉链基序结合的蛋白质。  |  Favier, D. and Gonda, TJ. 1994. Oncogene. 9: 305-11. PMID: 8302594

订购信息

产品名称产品编号规格价格数量收藏夹

c-Myb 抗体 (D-7)

sc-74512
200 µg/ml
$316.00

c-Myb (D-7): m-IgG Fc BP-HRP 套装

sc-528678
200 µg Ab; 10 µg BP
$354.00

c-Myb (D-7): m-IgGκ BP-HRP 套装

sc-521127
200 µg Ab, 40 µg BP
$354.00

c-Myb (D-7): m-IgG2a BP-HRP 套装

sc-547142
200 µg Ab; 10 µg BP
$354.00

c-Myb 抗体 (D-7) X

sc-74512 X
200 µg/0.1 ml
$316.00

c-Myb 抗体 (D-7) AC

sc-74512 AC
500 µg/ml, 25% agarose
$416.00

c-Myb 抗体 (D-7) HRP

sc-74512 HRP
200 µg/ml
$316.00

c-Myb 抗体 (D-7) FITC

sc-74512 FITC
200 µg/ml
$330.00

c-Myb 抗体 (D-7) PE

sc-74512 PE
200 µg/ml
$343.00

c-Myb 抗体 (D-7) Alexa Fluor® 488

sc-74512 AF488
200 µg/ml
$357.00

c-Myb 抗体 (D-7) Alexa Fluor® 546

sc-74512 AF546
200 µg/ml
$357.00

c-Myb 抗体 (D-7) Alexa Fluor® 594

sc-74512 AF594
200 µg/ml
$357.00

c-Myb 抗体 (D-7) Alexa Fluor® 647

sc-74512 AF647
200 µg/ml
$357.00

c-Myb 抗体 (D-7) Alexa Fluor® 680

sc-74512 AF680
200 µg/ml
$357.00

c-Myb 抗体 (D-7) Alexa Fluor® 790

sc-74512 AF790
200 µg/ml
$357.00

Dear Sir or Madam, Good Morning! Do you have FACS data for the anti-MYB (D7) antibody? Thanks!

Asked by: Xiubin
Thank you for your question. We do not have FACS data but we do have a 1year warranty from date of purchase.
Answered by: BlakeJ
Date published: 2024-05-22

Does this antibody recognize MYB isoforms with C-term truncations -- specifically is the ~52kd band a shorter isoform of MYB? Thanks!

Asked by: mybq
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-01-25

What is the recommended secondary for this antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-01-24
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Rated 5 out of 5 by from high positive rateI have a free sample and I using this antibody for immunoblotting. When we dilute with 1:100, the band is distinct.
Date published: 2017-10-26
Rated 5 out of 5 by from statisfied resultThis antibody specificly against the target protein c-Myb .Human colon cancer tissues sections shows strong staining (1:200),statisfied result.
Date published: 2017-09-09
Rated 5 out of 5 by from HL-60 whole cell lysatesUsed the antibody at 1:800 dilution for HL-60 whole cell lysates, give a strong band.
Date published: 2017-09-06
Rated 5 out of 5 by from suitable for WBThe antibody is dilution 1:500,used for WB. Works pretty well. The signal is very specific. Some background seen but can be optimized.
Date published: 2017-08-12
Rated 5 out of 5 by from successfully analysisThe direction of our laboratory research is esophageal cancer, we used c-myb antibodies for many years, we used this antibody published several articles,it good quality for WB and IF detection.
Date published: 2017-07-15
Rated 4 out of 5 by from Great antobody for immunoblotingThis antibody was used a a loading control for lysate from HEK293T cells. The antibody gives a strong single band at 1:1000 dilution.
Date published: 2017-05-12
Rated 5 out of 5 by from Great antibodyThis antibody also recognize the mouse c-Myb, and give specific signal in Western blot.
Date published: 2017-04-25
Rated 5 out of 5 by from recommend useWe used c-Myb Antibody Western blot analysis of c-Myb expression in rat spleen tissue extract,the effect is quite good.Recommended use.
Date published: 2017-03-10
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c-Myb 抗体 (D-7) is rated 4.9 out of 5 by 12.
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