c-Fms/CSF-1R Antibody (5J15): sc-70452

c-Fms/CSF-1R Antibody (5J15) is a rat monoclonal IgG1 antibody that detects c-Fms/CSF-1R in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. c-Fms/CSF-1R, also known as macrophage colony-stimulating factor receptor (M-CSFR), FIM2, or CD115, is a transmembrane tyrosine kinase receptor that plays a crucial role in the growth and differentiation of myeloid cells. This receptor is encoded by the c-Fms proto-oncogene and is predominantly expressed in mononuclear phagocytes, oocytes, decidual cells, trophoblastic cells, and certain myoblasts. The significance of c-Fms/CSF-1R lies in its ability to mediate the functions of macrophage colony-stimulating factor (M-CSF), a glycoprotein essential for the proliferation and differentiation of mononuclear phagocytes, including osteoclasts. M-CSF is also a key player in the inflammatory response, regulating the release of proinflammatory cytokines from macrophages, which underscores its importance in both immune response and tissue homeostasis. Anti-c-Fms/CSF-1R antibody (5J15) is an invaluable tool for researchers studying myeloid cell biology and the mechanisms underlying inflammation and immune regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

c-Fms/CSF-1R Antibody (5J15) References:

1. Regulation of urokinase plasminogen activator gene transcription in the RAW264 murine macrophage cell line by macrophage colony-stimulating factor (CSF-1) is dependent upon the level of cell-surface receptor.  |  Fowles, LF., et al. 2000. Biochem J. 347 Pt 1: 313-20. PMID: 10727433
2. Targeted disruption of the mouse colony-stimulating factor 1 receptor gene results in osteopetrosis, mononuclear phagocyte deficiency, increased primitive progenitor cell frequencies, and reproductive defects.  |  Dai, XM., et al. 2002. Blood. 99: 111-20. PMID: 11756160
3. Hormonal regulation of the c-fms proto-oncogene in breast cancer cells is mediated by a composite glucocorticoid response element.  |  Flick, MB., et al. 2002. J Cell Biochem. 85: 10-23. PMID: 11891846
4. Molecular cloning and expression analysis of a macrophage-colony stimulating factor receptor-like gene from rainbow trout, Oncorhynchus mykiss.  |  Honda, T., et al. 2005. Mol Immunol. 42: 1-8. PMID: 15488938
5. The colony-stimulating factor 1 receptor is expressed on dendritic cells during differentiation and regulates their expansion.  |  MacDonald, KP., et al. 2005. J Immunol. 175: 1399-405. PMID: 16034075
6. Expression of human CSF-1 receptor induces CSF-1-dependent proliferation in murine myeloid but not in T-lymphoid cells.  |  von Rüden, T., et al. 1991. Leukemia. 5: 3-7. PMID: 1825680
7. Regulation of colony stimulating factor-1 expression and ovarian cancer cell behavior in vitro by miR-128 and miR-152.  |  Woo, HH., et al. 2012. Mol Cancer. 11: 58. PMID: 22909061
8. Ligand and protein kinase C downmodulate the colony-stimulating factor 1 receptor by independent mechanisms.  |  Downing, JR., et al. 1989. Mol Cell Biol. 9: 2890-6. PMID: 2528680
9. Expression of human colony-stimulating factor-1 (CSF-1) receptor in murine pluripotent hematopoietic NFS-60 cells induces long-term proliferation in response to CSF-1 without loss of erythroid differentiation potential.  |  Bourette, RP., et al. 1993. Blood. 81: 2511-20. PMID: 7683918
10. Expression of functional human CSF-1 receptors on mouse bone marrow erythroid progenitor cells.  |  Mouchiroud, G., et al. 1993. C R Acad Sci III. 316: 1290-6. PMID: 8087610

The preferred c-Fms/CSF-1R antibody is c-Fms/CSF-1R Antibody (B-8): sc-46662.