Date published: 2025-9-11

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BrdU 抗体 (MoBU-1): sc-51514

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说明书
  • BrdU 抗体 MoBU-1 是小鼠单克隆 IgG1, 在25篇文献中引用,提供 100 µg/ml
  • 培养自结合血蓝蛋白的5-bromodeoxyuridine (BrdU)
  • 推荐用于 BrdU, a proliferation marker incorporated into newly synthesized DNA during S-phase of a cell cycle, IF, IHC(P) 和 FCM检测
  • 识别标记了BrdU的细胞的变性DNA中的BrdU
  • 推荐配套产品:5-Bromo-2′ deoxyuridine: sc-290815
  • 关于如何获取BrdU (MoBU-1): sc-51514免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP是BrdU Antibody (MoBU-1) 适用于 IHC(P) 应用。 的首选辅助检测试剂,这些试剂现与BrdU Antibody (MoBU-1) 打包提供(请参阅下面的订购信息)。

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関連項目

BrdU抗体(MoBU-1)是一种IgG1 κ小鼠单克隆BrdU抗体(也称为BrdU抗体或Bromodeoxyuridine抗体),可通过IF、IHC(P)和FCM检测细胞周期S期新合成DNA中植入的增殖标记物BrdU。BrdU抗体(MoBU-1)可以作为非偶联的抗BrdU抗体形式使用。溴代嘧啶胸腺嘧啶(BrdU)是一种卤代嘧啶同源物,可被植入S期细胞的新合成DNA链中,可用于估计S期细胞的比例。此外,BrdU的吸收分析是可靠的用于量化DNA合成程度的方法。BrdU也可用于研究姊妹染色体交换和分离新生DNA。紫外线诱导的消除修复合成是将BrdU植入细胞DNA的一种方法。抗BrdU抗体经过盐酸解离步骤或核酸酶酶切后,可结合单链DNA中暴露的BrdU。大多数细胞或组织固定于交联剂(如甲醛)时,需要蛋白酶抗原复原,但可能会降低BrdU免疫检测的特异性。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

BrdU 抗体 (MoBU-1)

sc-51514
100 µg/ml
$316.00

BrdU (MoBU-1): m-IgG Fc BP-HRP 套装

sc-536846
100 µg Ab; 10 µg BP
$354.00

BrdU (MoBU-1): m-IgGκ BP-HRP 套装

sc-534013
100 µg Ab; 40 µg BP
$354.00

Is it absolutely necessary to do a denaturing step to visualize the antibody by IF in paraffin sections? By treating the sections with 2M HCl, I am unable to see a proper DAPI staining and therefore cannot quantify the PERCENTAGE of BrdU positive cells.

Asked by: Mirabai
Thank you for your message. Please find on the following link our highly recommended protocol: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining If you have further questions, please do not hesitate to contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2021-08-17

What is the total volume provided of BrdU (MoBU-1): sc-51514 monoclonal antibody?

Asked by: cjMara
Thank you for your question. This antibody is provided as 100 µg in 1 ml total volume.
Answered by: Technical Support
Date published: 2017-12-04
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Rated 5 out of 5 by from Very Good IHC antibodyThis antibody consistently worked well for us on FFPE mouse small intestinal tissue.
Date published: 2020-06-27
Rated 5 out of 5 by from Reliable Antibody, Great for IHCThis antibody is great for producing images of dividing cells. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-18
Rated 5 out of 5 by from useful in scientific researchi bought BrdU antibody for my WB experiment ,it makes good result ,i recommand it
Date published: 2016-12-05
Rated 5 out of 5 by from IHC publishable data on rat sciatic nerveIHC publishable data on rat sciatic nerve - SCBT Publication Review
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Date published: 2013-02-09
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BrdU 抗体 (MoBU-1) is rated 5.0 out of 5 by 6.
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