Date published: 2025-9-6
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BID Antibody (B-3) - Western Blotting - Image 361489
BID 抗体 (B-3): sc-373939. 对 HeLa (A) 和 Jurkat (B) 全细胞裂解液中 BID 表达的 Western 印迹分析.
BID 抗体 (B-3): sc-373939. 对 HeLa (A) 和 Jurkat (B) 全细胞裂解液中 BID 表达的 Western 印迹分析.

BID 抗体 (B-3): sc-373939

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说明书
  • BID 抗体 B-3 是小鼠单克隆 IgG1 κ，BID抗体, 在24篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长BID的氨基酸1-195
  • BID 抗体 (B-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 BID
  • 抗BID抗体（B-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取BID (B-3): sc-373939的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 BID 抗体 (B-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与BID 抗体 (B-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

BID抗体（B-3）是一种IgG1 κ小鼠单克隆BID抗体（也称为BH3相互作用域死亡激动剂抗体），可通过蛋白质印迹法（WB）、免疫沉淀法（IP）、免疫荧光法（IF）、免疫组织化学法（IHC-P）和酶联免疫吸附试验（ELISA）检测人类的BID蛋白。BID抗体（B-3）既有非偶联的抗BID抗体形式，也有多种偶联的抗BID抗体形式，包括琼脂糖、辣根过氧化物酶（HRP）、PE、FITC和多种Alexa Fluor®偶联物。Bcl-2蛋白家族的成员相互作用以调节程序性细胞死亡或凋亡。该家族中的蛋白质形成的各种同源二聚体和异源二聚体可以促进或抑制凋亡。Bcl-2阻断多种刺激后的细胞死亡，并在撤除生长因子后对某些造血细胞系产生死亡保护作用。该家族中的其他凋亡抑制剂包括A1、Bag-1、Bcl-w、Bcl-x和Mcl-1。该家族的促凋亡成员包括Bax、Bad、Bak、Bik（NBK）和BID。BID包含一个BH3结构域，使其能够与Bcl-2二聚化并抵消其死亡抑制效应。BID还被证明与Bcl-x和死亡激动剂Bax异源二聚化。BID主要富集在细胞质中，也存在于膜组分中。它在肾脏中高表达，也可以在脑、脾、肝、睾丸和肺中检测到。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BID 抗体 (B-3) 参考文献:

  1. 肾细胞在体外ATP耗竭和体内缺血后激活Bid。  |  Wei, Q., et al. 2004. Am J Physiol Renal Physiol. 286: F803-9. PMID: 14678945
  2. Bcl-2 基因表达失调可选择性地延长缺乏生长因子的造血细胞系的存活时间。  |  Nuñez, G., et al. 1990. J Immunol. 144: 3602-10. PMID: 2184193
  3. BID与阿霉素协同杀死癌细胞。  |  Orzechowska, EJ., et al. 2015. Oncol Rep. 33: 2143-50. PMID: 25760094
  4. 肝脏特异性Bid沉默抑制小鼠中APAP诱导的细胞死亡。  |  Maxa, M., et al. 2019. Apoptosis. 24: 934-945. PMID: 31576482
  5. Bcl-2 基因促进造血细胞存活，并与 c-myc 合作使前 B 细胞永生。  |  Vaux, DL., et al. 1988. Nature. 335: 440-2. PMID: 3262202
  6. 用酵母双杂交系统分析 Bcl-2 蛋白家族成员之间的相互作用。  |  Sato, T., et al. 1994. Proc Natl Acad Sci U S A. 91: 9238-42. PMID: 7937747
  7. 决斗二聚体的检查站挫败了死亡愿望。  |  Oltvai, ZN. and Korsmeyer, SJ. 1994. Cell. 79: 189-92. PMID: 7954787
  8. Bcl-2 在体内与保守的同源物 Bax 异源二聚体，从而加速细胞的程序性死亡。  |  Oltvai, ZN., et al. 1993. Cell. 74: 609-19. PMID: 8358790
  9. 分子比拟：关于 BCL2 家族和细胞死亡的论述。  |  Yang, E. and Korsmeyer, SJ. 1996. Blood. 88: 386-401. PMID: 8695785
  10. BID：一种新型纯 BH3 结构域死亡激动剂。  |  Wang, K., et al. 1996. Genes Dev. 10: 2859-69. PMID: 8918887
  11. 死亡因子导致的细胞凋亡  |  Nagata, S. 1997. Cell. 88: 355-65. PMID: 9039262
  12. 干扰素-α2b诱导肿瘤前大鼠肝脏离体肝细胞凋亡的发生与时间有关。  |  Alvarez, Mde L., et al. 2006. Cytokine. 36: 245-53. PMID: 17376698

订购信息

产品名称产品编号规格价格数量收藏夹

BID 抗体 (B-3)

sc-373939
200 µg/ml
$316.00

BID (B-3): m-IgG Fc BP-HRP 套装

sc-529614
200 µg Ab; 10 µg BP
$354.00

BID (B-3): m-IgGκ BP-HRP 套装

sc-522562
200 µg Ab, 40 µg BP
$354.00

BID (B-3): m-IgG1 BP-HRP 套装

sc-543606
200 µg Ab; 20 µg BP
$354.00

BID 抗体 (B-3) AC

sc-373939 AC
500 µg/ml, 25% agarose
$416.00

BID 抗体 (B-3) HRP

sc-373939 HRP
200 µg/ml
$316.00

BID 抗体 (B-3) FITC

sc-373939 FITC
200 µg/ml
$330.00

BID 抗体 (B-3) PE

sc-373939 PE
200 µg/ml
$343.00

BID 抗体 (B-3) Alexa Fluor® 488

sc-373939 AF488
200 µg/ml
$357.00

BID 抗体 (B-3) Alexa Fluor® 546

sc-373939 AF546
200 µg/ml
$357.00

BID 抗体 (B-3) Alexa Fluor® 594

sc-373939 AF594
200 µg/ml
$357.00

BID 抗体 (B-3) Alexa Fluor® 647

sc-373939 AF647
200 µg/ml
$357.00

BID 抗体 (B-3) Alexa Fluor® 680

sc-373939 AF680
200 µg/ml
$357.00

BID 抗体 (B-3) Alexa Fluor® 790

sc-373939 AF790
200 µg/ml
$357.00

hi how are you which is the better dultion for bid rab antiobody ,and secondary antiobody for this protien

Asked by: bio2223
Thank you for your question. The recommended dilution for primary and secondary antibody depends on the application you intend to use it for. Our recommended starting dilution for the primary antibody for Western Blot, for example, 1:200, but for IF or IHC would be 1:50. The secondary should be used at the manufacturer's recommended range. All of our recommended protocols can be found on the website here: https://www.scbt.com/scbt/resources/protocols Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-05-04

Can BID (B-3): sc-373939 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, BID (B-3): sc-373939 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Ottimo funzionamentoRicevuto un campione dell'anticorpo è stato utilizzato sciogliendolo secondo la sheet, ed è risultato funzionante. La banda si vede bene.
Date published: 2020-11-09
Rated 4 out of 5 by from Good antibodyI tried this antibody to replace the reference sc-11423 Bid (FL195) which reconizes FL-BID and t-BID in our model. Works on Burkitt Lymphoma cells but unfortunatly it doesn't reconize the truncated version of Bid.
Date published: 2017-11-09
Rated 5 out of 5 by from 条带很清晰刚拿到这个产品一周，在293T细胞上进行内源性检测，1:200进行稀释，没有非特异性条带，效果很好。
Date published: 2017-08-31
Rated 5 out of 5 by from Great feutureGreat specificity bands at accurate size for mouse cell lysate.
Date published: 2017-08-21
Rated 5 out of 5 by from Great bands seen in WB using U138Great bands seen in WB using U138 glioma cell line
Date published: 2017-07-28
Rated 5 out of 5 by from Excellent AbUsed in Western blot. Produced a single band at the correct size.
Date published: 2017-03-29
Rated 5 out of 5 by from Great antibody to detect Bid expressionBeautiful bands can be seen in Western Blot assay using L929 whole cell lysates.
Date published: 2016-11-04
Rated 5 out of 5 by from WB publishable data with human lung cancer H441WB publishable data with human lung cancer H441 - SCBT Publication Review
Date published: 2015-05-02
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BID 抗体 (B-3) is rated 4.6 out of 5 by 10.
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