Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
beta Tubulin Antibody (D-10): sc-5274. Western blot analysis of β Tubulin expression in A-431 (A), BJAB (B), NIH/3T3 (C) and KNRK (D) whole cell lysates.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
beta Tubulin CRISPR/Cas9 KO Plasmid (h): sc-400044
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- beta Tubulin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the beta Tubulin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- beta Tubulin HDR Plasmid (h) (sc-400044-HDR) is recommended for co-transfection with beta Tubulin CRISPR/Cas9 KO Plasmid (h) to enable selection of successfully edited cells through HDR-mediated integration of a puromycin resistance cassette and RFP reporter gene
- beta Tubulin HDR Plasmid (h) is a pool of plasmids, each containing a homology-directed repair (HDR) template corresponding to the gRNA target sites in the beta Tubulin CRISPR/Cas9 KO Plasmid (h)
- Each HDR plasmid contains two ~800 bp homology arms flanking the puromycin resistance and RFP cassettes, designed to bind genomic DNA sequences surrounding the Cas9-induced double-strand break site and facilitate precise HDR-mediated integration
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: beta Tubulin Antibody (D-10): sc-5274
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
beta Tubulin CRISPR/Cas9 KO Plasmid (h) | sc-400044 | 20 µg | $397.00 | |||
beta Tubulin HDR Plasmid (h) | sc-400044-HDR | 20 µg | $445.00 |