Date published: 2025-9-6
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beta Tubulin Antibody (D-10) - Western Blotting - Image 394014
β 管蛋白抗体 (D-10) Alexa Fluor® 790: sc-5274 AF790. 直接近红外 Western 印迹分析 A-431 (A), BJAB (B), K-562 (C), U-251-MG (D) 和 U-2 OS (E) 全细胞裂解液中 β 管蛋白的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
β 管蛋白抗体 (D-10) Alexa Fluor® 790: sc-5274 AF790. 直接近红外 Western 印迹分析 A-431 (A), BJAB (B), K-562 (C), U-251-MG (D) 和 U-2 OS (E) 全细胞裂解液中 β 管蛋白的表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

beta Tubulin 抗体 (D-10): sc-5274

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寫評論提問

说明书
  • beta Tubulin 抗体 D-10 是小鼠单克隆 IgG2b κ，beta Tubulin抗体, 在585篇文献中引用，规格为200 µg/ml
  • 与重组蛋白结合，该重组蛋白与β Tubulin的210-444的氨基酸对应，来源于human
  • beta Tubulin 抗体 (D-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 β Tubulin
  • 抗beta Tubulin抗体（D-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取beta Tubulin (D-10): sc-5274的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 是beta Tubulin 抗体 (D-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与beta Tubulin 抗体 (D-10) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Tubulin 抗体 包括 α 1 Tubulin, α 3 Tubulin, α 4 Tubulin, α Tubulin, β Tubulin, β 2 Tubulin, β 3 Tubulin, δ Tubulin, ε Tubulin和γ Tubulin

β 管蛋白抗体（D-10）是一种小鼠单克隆IgG2b kappa轻链抗体，经过工程改造，可识别小鼠、大鼠和人类中的β 管蛋白（也称为管蛋白β 链）。β Tubulin 单克隆抗体（D-10）针对与人类β Tubulin 氨基酸 210-444 相对应的重组蛋白进行免疫，确保在应用中（如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光 、免疫组织化学和酶联免疫吸附测定（ELISA）等应用中实现精确结合和卓越性能。β-微管蛋白是细胞骨架的基本组成部分，在维持细胞结构、促进细胞内运输以及通过微管的形成和稳定实现细胞分裂方面发挥着关键作用。β-微管蛋白有多种同工型，包括β1、β2、β3、β4、β5、β6和β8，每种同工型都有独特的功能和细胞定位，例如神经元特异性β3同工型。这种多样性使得β-微管蛋白能够参与各种细胞过程，使其成为神经生物学、癌症和细胞生物学等领域研究的重要目标。了解β-微管蛋白的功能对于阐明细胞运动、分裂和细胞内组织机制至关重要，这些机制对于正常生理和疾病状态都是至关重要的。β Tubulin (D-10) 抗体有非结合型和多种结合型，包括辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合物，为不同的实验设置提供了多功能性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

beta Tubulin 抗体 (D-10) 参考文献:

  1. 培养的大鼠肾间质细胞核中存在βII异型小管蛋白。  |  Walss, C., et al. 1999. Cell Motil Cytoskeleton. 42: 274-84. PMID: 10223634
  2. 鉴定大西洋鳕鱼（Gadus morhua）冷适应微管中的βIII-和βIV-微管蛋白异型：抗体图谱和 cDNA 测序。  |  Modig, C., et al. 1999. Cell Motil Cytoskeleton. 42: 315-30. PMID: 10223637
  3. Delta-tubulin和epsilon-tubulin：两种新的人类中心体管蛋白揭示了中心体结构和功能的新方面。  |  Chang, P. and Stearns, T. 2000. Nat Cell Biol. 2: 30-5. PMID: 10620804
  4. 人脑中具有管蛋白免疫反应的神经元核内包涵体。  |  Woulfe, J. and Munoz, D. 2000. Neuropathol Appl Neurobiol. 26: 161-71. PMID: 10840279
  5. α-, β-和γ-管蛋白：序列比较和结构限制。  |  Burns, RG. 1991. Cell Motil Cytoskeleton. 20: 181-9. PMID: 1773446
  6. γ-微管蛋白存在于黑腹果蝇和智人体内，与中心体有关。  |  Zheng, Y., et al. 1991. Cell. 65: 817-23. PMID: 1904010
  7. 特约评论：三磷酸核苷酸在肌动蛋白和微管蛋白组装和功能中的作用。  |  Weisenberg, RC. 1981. Cell Motil. 1: 485-97. PMID: 6756643
  8. 多种形式的微管蛋白：不同的基因产物和共价修饰。  |  Ludueña, RF. 1998. Int Rev Cytol. 178: 207-75. PMID: 9348671
  9. 氨基端和羧基端γ-和α-微管蛋白突变体在培养上皮细胞中的表达。  |  Leask, A. and Stearns, T. 1998. J Biol Chem. 273: 2661-8. PMID: 9446570

订购信息

产品名称产品编号规格价格数量收藏夹

beta Tubulin 抗体 (D-10)

sc-5274
200 µg/ml
$316.00

beta Tubulin (D-10): m-IgG2b BP-HRP 套装

sc-548642
200 µg Ab; 10 µg BP
$354.00

beta Tubulin 抗体 (D-10) AC

sc-5274 AC
500 µg/ml, 25% agarose
$416.00

beta Tubulin 抗体 (D-10) HRP

sc-5274 HRP
200 µg/ml
$316.00

beta Tubulin 抗体 (D-10) FITC

sc-5274 FITC
200 µg/ml
$330.00

beta Tubulin 抗体 (D-10) PE

sc-5274 PE
200 µg/ml
$343.00

beta Tubulin 抗体 (D-10) Alexa Fluor® 488

sc-5274 AF488
200 µg/ml
$357.00

beta Tubulin 抗体 (D-10) Alexa Fluor® 546

sc-5274 AF546
200 µg/ml
$357.00

beta Tubulin 抗体 (D-10) Alexa Fluor® 594

sc-5274 AF594
200 µg/ml
$357.00

beta Tubulin 抗体 (D-10) Alexa Fluor® 647

sc-5274 AF647
200 µg/ml
$357.00

beta Tubulin 抗体 (D-10) Alexa Fluor® 680

sc-5274 AF680
200 µg/ml
$357.00

beta Tubulin 抗体 (D-10) Alexa Fluor® 790

sc-5274 AF790
200 µg/ml
$357.00

beta Tubulin 抗体 (D-10) B

sc-5274 B
200 µg/ml
$320.00

What primary antibody dilution solution you recommend to use this antibody (beta Tubulin Anticuerpo (D-10): sc-5274)? 1X TBST with 5% w/v nonfat dry milk, maybe??

Asked by: Laum94
We recommend a starting dilution of 1:100 for our primary antibodies for western blot.
Answered by: Technical Service
Date published: 2024-05-07

What is the lower band (~33kDa) in the western blot image shown on this webpage for sample (D)-KNRK whole cell lysate? I observed this band in my HeLa whole cell lysate as well.

Asked by: Kayla08
Thank you for your question. Unfortunately this is not a band that we consistently see in our in house testing, so we have not looked into this non-specific band. We do not see it in Hela cells, so I am not sure what you could be seeing. The antibody does not cross-react with anything that should run at that size. Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2020-01-23

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2023-05-03

Which isoform of beta tubulin does this antibody recognize?

Asked by: eleni
Thank you for your question. Unfortunately the exact epitope of this antibody has not been mapped. It is raised against a recombinant protein corresponding to amino acids 210-444 of β Tubulin of human origin, accession#P07437. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-05-09
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Rated 5 out of 5 by from goodthis antibody works well in skeletal muscle of mice
Date published: 2023-05-29
Rated 4 out of 5 by from A good antibody to stain microtubules in cells.Good quality for immunostaining ad immunoblotting. Convenient formula (no freeze, no aliquot) for daily operation.
Date published: 2023-01-15
Rated 5 out of 5 by from Excellent western blotting antibodiesI am very happy to mention that we used your antibodies of beta-actin, caspase 3, 9, 10, and SOD for western blotting. at a minimum concentration of 1: 5k all the antibodies worked fantastically. Thank you so much for the excellent products. We will continue to bussiness with you!
Date published: 2021-12-20
Rated 5 out of 5 by from 抗体没有申请试用装，看了评价打分挺高的，就直接购买了！所以大家一定要实事求是啊，否则就不地道了！用了还不错。
Date published: 2021-07-22
Rated 5 out of 5 by from It works very nicelyWe have used this antibody in the lab for 4 years, and it has always worked nicely. We use a 1:3000 dilution.
Date published: 2021-04-02
Rated 5 out of 5 by from beta-tubulin Ab for WBExcellent antibody for WB dilution 1:50000 good for housekeeping protein of colon cancer cell lines
Date published: 2021-03-23
Rated 2 out of 5 by from discreto funzionamentoQuesto anticorpo funziona discretamente su cellule fissate con metanolo freddo (-20°C); non funziona in cellule fissate in formalina
Date published: 2021-03-18
Rated 5 out of 5 by from Great b-Tubulin antibody for WesternThis is a very good mouse monoclonal Ab for beta-Tubulin. We used it with 1:500 dilution and results were very satisfactory and clean. I recommend this antibody.
Date published: 2019-07-30
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beta Tubulin 抗体 (D-10) is rated 4.7 out of 5 by 87.
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