Date published: 2025-9-7
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β-TrCP/HOS Antibody (F-10) - Western Blotting - Image 81195
β-TrCP/HOS (F-10): sc-166492. 非转染: sc-117752 (A) 和人 HOS 转染: sc-113730 (B) 293T 全细胞裂解液中 HOS 表达的 Western 印迹分析.
β-TrCP/HOS Antibody (F-10) - Western Blotting - Image 81195
β-TrCP/HOS (F-10): sc-166492. 非转染: sc-117752 (A) 和人 HOS 转染: sc-113730 (B) 293T 全细胞裂解液中 HOS 表达的 Western 印迹分析.
β-TrCP/HOS Antibody (F-10) - Western Blotting - Image 143331
β-TrCP/HOS (F-10): sc-166492. 非转染: sc-117752 (A) 和人 HOS 转染: sc-113730 (B) 293T 全细胞裂解液中 HOS 表达的 Western 印迹分析.
β-TrCP/HOS (F-10): sc-166492. 非转染: sc-117752 (A) 和人 HOS 转染: sc-113730 (B) 293T 全细胞裂解液中 HOS 表达的 Western 印迹分析.

β-TrCP/HOS 抗体 (F-10): sc-166492

3.5(8)
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说明书
  • beta-TrCP/HOS 抗体 F-10 是小鼠单克隆 IgG1 κ，β-TrCP/HOS抗体, 在8篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的β-TrCP/HOS的C-terminus的氨基酸531-550之间
  • beta-TrCP/HOS 抗体 (F-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 β-TrCP and HOS
  • 抗beta-TrCP/HOS抗体（F-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取β-TrCP/HOS (F-10): sc-166492的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是β-TrCP/HOS Antibody (F-10) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与β-TrCP/HOS Antibody (F-10) 打包提供（请参阅下面的订购信息）。

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関連項目

附加β-TrCP抗体，包括β-TrCP/HOS

β-TrCP/HOS抗体（F-10）是一种IgG1 κ小鼠单克隆β-TrCP/HOS抗体（也被指定为包含E3泛素蛋白连接酶1/2的抗体的β-转导素重复，BTRC抗体，BTRC2抗体，BTRCP1抗体，BTRCP2抗体，βTrCP1抗体，βTrCP2抗体，含F-box/WD重复序列的蛋白质1A（FBXW1A）抗体，FBW1A抗体，FBXW1抗体，FBXW1B抗体，FBXW11抗体，betaTrCP抗体，泛素蛋白连接酶复合物亚基抗体，或磷酸化依赖性泛素途径相关抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的β-TrCP/HOS蛋白。β-TrCP/HOS抗体（F-10）既可以作为非共轭的抗β-TrCP/HOS抗体形式，也可以作为多种共轭形式的抗β-TrCP/HOS抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。含有β-tranducin重复序列的蛋白（β-TrCP），也被指定为E3RSIkB或FWD1，和HOS（与Slimb同源）是F-box蛋白，作为泛素连接酶的底物识别亚基发挥作用。HOS和b-TrCP在它们的氨基末端区域不同，但在F-box和包含WD40重复序列的区域内表现出高度同源性。β-TrCP介导CD4的泛素/蛋白酶体依赖性降解以及各种蛋白质（包括IkB和β-catenin）的泛素化。HOS也被证明以类似的方式调节IkB和β-catenin的降解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

β-TrCP/HOS 抗体 (F-10) 参考文献:

  1. IkappaBalpha 的泛素依赖性降解是由泛素连接酶 Skp1/Cul 1/F-box 蛋白 FWD1 介导的。  |  Hatakeyama, S., et al. 1999. Proc Natl Acad Sci U S A. 96: 3859-63. PMID: 10097128
  2. HOS 是 Slimb 的人类同源物，它与 Skp1 和 Cullin1 形成 SCF 复合物，靶向磷酸化依赖性降解 IkappaB 和 beta-catenin。  |  Fuchs, SY., et al. 1999. Oncogene. 18: 2039-46. PMID: 10321728
  3. Lats和CK1的协调磷酸化通过SCF（β-TRCP）调节YAP的稳定性。  |  Zhao, B., et al. 2010. Genes Dev. 24: 72-85. PMID: 20048001
  4. 环状 RNA circPVT1 通过 β-TrCP/c-Myc/SRSF1 正反馈环促进鼻咽癌转移  |  Mo, Y., et al. 2022. Mol Cancer. 21: 192. PMID: 36199071
  5. SDCBP 通过阻止 YAP1 在 β-TrCP 介导下被蛋白酶体降解，从而促进胰腺癌的进展。  |  Liu, J., et al. 2023. Gut. 72: 1722-1737. PMID: 36828627
  6. 人脐源性间充质干细胞移植添加姜黄素可通过AKT/GSK-3β/β-TrCP/Nrf2轴改善缺血性中风的预后。  |  Li, Y., et al. 2023. J Neuroinflammation. 20: 49. PMID: 36829224
  7. 全反式维甲酸促进食管鳞状细胞癌的抑瘤OTUD6B-β-TrCP-SNAIL轴并增强免疫疗法  |  Li, L., et al. 2023. Adv Sci (Weinh). 10: e2207458. PMID: 37038094
  8. 隐藏在环形 RNA 中的新型 β-TrCP 蛋白异构体使 HER2 阳性乳腺癌产生曲妥珠单抗抗药性。  |  Wang, S., et al. 2023. Redox Biol. 67: 102896. PMID: 37783059
  9. 抑制 MST1 可通过 GSK3β/β-TrCP/NRF2 通路改善糖尿病伴脊髓损伤的神经细胞凋亡。  |  Huang, W., et al. 2024. Redox Biol. 71: 103104. PMID: 38430683
  10. 一种新型人类 WD 蛋白--h-beta TrCp--能与 HIV-1 Vpu 相互作用，通过 F-box motif 将 CD4 连接到 ER 降解途径。  |  Margottin, F., et al. 1998. Mol Cell. 1: 565-74. PMID: 9660940
  11. SCF 泛素蛋白连接酶底物识别亚基的泛素化和降解。  |  Zhou, P. and Howley, PM. 1998. Mol Cell. 2: 571-80. PMID: 9844630
  12. 鉴定 IkappaBalpha-泛素连接酶的受体成分。  |  Yaron, A., et al. 1998. Nature. 396: 590-4. PMID: 9859996

订购信息

产品名称产品编号规格价格数量收藏夹

β-TrCP/HOS 抗体 (F-10)

sc-166492
200 µg/ml
$316.00

β-TrCP/HOS (F-10): m-IgG1 BP-HRP 套装

sc-531680
200 µg Ab; 20 µg BP
$354.00

β-TrCP/HOS 抗体 (F-10) AC

sc-166492 AC
500 µg/ml, 25% agarose
$416.00

β-TrCP/HOS 抗体 (F-10) HRP

sc-166492 HRP
200 µg/ml
$316.00

β-TrCP/HOS 抗体 (F-10) FITC

sc-166492 FITC
200 µg/ml
$330.00

β-TrCP/HOS 抗体 (F-10) PE

sc-166492 PE
200 µg/ml
$343.00

β-TrCP/HOS 抗体 (F-10) Alexa Fluor® 488

sc-166492 AF488
200 µg/ml
$357.00

β-TrCP/HOS 抗体 (F-10) Alexa Fluor® 546

sc-166492 AF546
200 µg/ml
$357.00

β-TrCP/HOS 抗体 (F-10) Alexa Fluor® 594

sc-166492 AF594
200 µg/ml
$357.00

β-TrCP/HOS 抗体 (F-10) Alexa Fluor® 647

sc-166492 AF647
200 µg/ml
$357.00

β-TrCP/HOS 抗体 (F-10) Alexa Fluor® 680

sc-166492 AF680
200 µg/ml
$357.00

β-TrCP/HOS 抗体 (F-10) Alexa Fluor® 790

sc-166492 AF790
200 µg/ml
$357.00

β-TrCP/HOS (F-10) 中和勝肽

sc-166492 P
100 µg/0.5 ml
$68.00

Is this antibody detects specifically bTrCP2/HOS/FBXW11? Does is'nt cross reacts with bTrCP1/FBXW1. Please clarify. Thank you

Asked by: Sehban
Thank you for your question. β-TrCP/HOS Antibody (F-10): sc-166492 is specific for an epitope mapping between amino acids 531-550 at the C-terminus of HOS of human origin. As this region shows little homology with its equivalent in bTrCP1/FBXW1 it is unlikely that the antibody would recognize this protein. Please contact our technical service for further information or to arrange for a free sample of β-TrCP/HOS Antibody (F-10): sc-166492 to try it in your application.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What application is the blocking peptide sc-166492 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good for Western BlotI tested this antibody on a HEK293 whole cell lysate, using it 1:1000 in 0,5% milk and obtaining a specific band at the expected molecular weight. I recommend this product for WB analysis
Date published: 2024-10-07
Rated 3 out of 5 by from GoodAcceptable band in WB analysis of b-Trcp expression.
Date published: 2017-11-24
Rated 3 out of 5 by from The band is a little weak1:200 diluted for wb of rat protein ,the band is a little weak.
Date published: 2017-09-16
Rated 3 out of 5 by from A fair antibodyAlthough this antibody did detect the target protein with expected molecular weight but also detect some other bands.
Date published: 2017-06-30
Rated 3 out of 5 by from performance in HCC cell lineI tested the sample beta-TrCP/HOS antibody in Huh7 and SK-Hep1 cell lines. Both cells were untreated or transfected with pEGFP-N1/ overexpressing plasmid. In all the groups, I could only observe blurry stripes when I loaded up to 40μg protein in western blot result (Bio-Rad Clarity Western ECL).
Date published: 2017-06-07
Rated 2 out of 5 by from Could not detect in Western blotWe could not detect endogenous protein in Western blot with whole cell lysate of T47D cells
Date published: 2017-03-14
Rated 5 out of 5 by from human pancreas tissueProduced nice immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue,the experimental results are very satisfactory.
Date published: 2016-12-17
Rated 5 out of 5 by from Great Western Blot data of HOS expression in nonGreat Western Blot data of HOS expression in non-transfected and human HOS transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-04-17
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β-TrCP/HOS 抗体 (F-10) is rated 3.5 out of 5 by 8.
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